Touchdown PCR for the detection of waterfowl parvoviruses

• Autorzy: Wozniakowski G , Kozdrun W. , Samorek-Salamonowicz E. , Krol K.
• Języki publikacji: EN

Abstrakty

EN

Sixteen field strains of goose parvovirus (GPV) and DNA extracted from standard strain of Muscovy duck parvovirus (MDPV) were used. PCR was used for the amplification of the VP3 structural protein encoding region. In the case of all GPV strains and one MDPV strain the presence of specific product about 1,604 bp long was observed. The essential conditions, which had the influence on the specificity, sensitivity, and efficiency of the amplification reaction, were optimised. In order to eliminate unspecific interactions between template and primers touchdown PCR was applied. Additionally, for specificity and efficiency improvement, betaine (N, N, N - trimethylglycine) was added. The conducted optimisation steps of PCR allowed for the identification of the both parvoviruses.

Słowa kluczowe

EN

polymerase chain reaction; identification; Parvoviridae; waterfowl; parvovirus; goose parvovirus; Dependovirus; Muscovy duck parvovirus; veterinary medicine; Carina moschata; Derzsy's disease; animal disease

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 1
• Opis fizyczny: p.3-7,fig.,ref.

Twórcy

autor

Wozniakowski G

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kozdrun W.

autor

Samorek-Salamonowicz E.

autor

Krol K.

Bibliografia

• 1. Baskaran N., Kandpal R.P., Bhargava A.K., Glynn M.W., Bale A., Weissman S.M.: Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content. Genome Res 1996, 6, 633-638.
• 2. Chang P.C., Shien J.H., Wang M. S., Shieh H.K.: Phylogenetic analysis of parvoviruses isolated in Taiwan from ducks and geese. Avian Pathol 2000, 29, 45-49.
• 3. Cotmore S.F., Tattersall P.: Structure and organization of the viral genome. In: Parvoviruses. Edited by Kerr J.R., Cotmore S.F., Bloom M.E., Linden R.M., Parrish C.R., Oxford University Press, Oxford 2006, pp. 73-94.
• 4. Don R.H., Cox P.T., Wainwright B.J., Baker K., Mattick J.S.: Touchdown PCR to circumvent spurious priming during gene amplification. Nucl Acids Res 1991, 19, 4008.
• 5. Henke W., Herdel K., Jung K., Schnorr D., Loening S.A.: Betaine improves the PCR amplification of GC-rich DNA sequences. Nucl Acids Res 1997, 25, 3957-3958.
• 6. Jestin V., Le Gall-Recule G., Arnauld C., Cherbonnel M., Jestin A.: Detection of Muscovy duck parvovirus DNA by the polymerase chain reaction. Immunology of viral infections. In: Proceedings of the 3rd Congress of the European Society for Veterinary Virology, Interlaken, Switzerland 1994. Edited by Schwyzer M., Ackermann M., Bertoni C., Kocherhans R., McCullough K., Engels M., Wittek R., Zanoni R., 1994, pp. 528-532.
• 7. Limn C., Yamada T., Nakamura M., Takehara K.: Detection of goose parvovirus genome by polymerase chain reaction: distribution of goose parvovirus in Muscovy ducklings. Virus Res 1996, 42, 167-172.
• 8. Kisary J., Derzsy D.: Viral disease of goslings. IV. Characterization of the causal agent in tissue culture system. Acta Vet Acad Sci Hung 1974, 24, 287-292.
• 9. Poonia B., Dunn P.A., Lu H., Jarosiński K.W., Schat K.A.: Isolation and molecular characterization of a new Muscovy duck parvovirus from Muscovy ducks in the USA. Avian Pathol 2006, 35, 435-441.
• 10. Roszkowski J., Gaździński P., Kozaczyński W.: Application of immunoperoxidase technique for detection of Derzsy's disease virus antigen in cell culture and gooslings. Avian Pathol 1982, 11, 571-578.
• 11. Sarkar G., Kapelner S., Sommer S.S.: Formamide can dramatically improve the specificity of PCR. Nuci Acids Res 1990, 18, 7465.
• 12. Tsai H.J., Tseng C.H., Chang P.C., Mei K., Wang S.C.: Genetic variation of viral protein 1 genes of field strains of waterfowl parvoviruses and their attenuated derivatives. Avian Dis 2004, 48, 512-521.
• 13. Woolcock P.R., Jestin V., Shivaprasad F., Zwingelstein F., Arnauld C., Mcfarland M. D., Pedersen J. C., Senne D. A.: Evidence of Muscovy duck parvovirus in Muscovy ducklings in California. Vet Rec 2000 146, 68-72.
• 14. Xing Z., Schat K.A.: Expression of cytokine genes in Marek's disease virus-infected chickens and chicken embryo fibroblasts. Immunology 2000, 100, 70-76.
• 15. Zadori Z., Erdei J., Nagy J., Kisary J.: Characteristics of the genome of goose parvovirus. Avian Pathol 1994, 23, 159-364.
• 16. Zádori Z., Stefancsik R., Rauch T., Kisary J.: Analysis of the complete nucleotide sequences of goose and muscovy duck parvoviruses indicates common ancestral origin with adeno-associated virus 2. Virology 1995, 212, 562- 573.