Host-specific in vitro colonisation of fish epithelia by gyrodactylids

• Autorzy: Larsen T B , Buchmann K.
• Języki publikacji: EN

Abstrakty

EN

Background. The monogeneans, Gyrodactylus derjavini Mikailov, 1975 and G. salaris Malmberg, 1957, are specific parasites of brown trout, Salmo trutta L., and Atlantic salmon, S. salar L., respectively. Rainbow trout, Oncorhynchus mykiss (Walbaum, 1792), is also susceptible to G. derjavini but, similarly like brown trout, mounts a host response against this parasite. Rainbow trout can also experience infections with G. salaris but experiences generally lower infection levels compared to Atlantic salmon. This in vitro work was conducted in order to elucidate if the parasites could find and select specific host scales without influence of live and mobile hosts. Materials and Methods. Using these two parasite species, a series of in vitro studies on host specificity were conducted. Scales with intact epithelia from rainbow trout, carp and salmon were cultivated and the two congeneric gyrodactylids (Gyrodactylus derjavini and G. salaris) were allowed to colonise the scales. Parasites attaching to the different scale types were monitored over 24 h. Results. The gyrodactylids colonised fish scales to a lesser extent, in vitro, compared to experiments where live fish were exposed to parasites. However, the parasites attached to a higher extent to in vitro-cultured fish scales with epithelia from their specific hosts: G. salaris relocated more often to salmon scales compared to the scales from rainbow trout and common carp, Cyprinus carpio L. In contrast, G. derjavini colonised preferentially cultured scales from rainbow trout and, to a lesser degree, scales from salmon. Conclusion. The work indicated that gyrodactylids colonising host epithelia are at least partly dependent on host movements but also have an ability to sense host-specific molecules due to their specific attachment to specific host scales.

• Wydawca: -
• Czasopismo: Acta Ichthyologica et Piscatoria
• Rocznik: 2006
• Tom: 36
• Numer: 2
• Opis fizyczny: p.113-118,fig.,ref.

Twórcy

autor

Larsen T B

• Royal Veterinary and Agricultural University, Stighojlen 7, DK-1870, Frederiksberg, Copenhagen, Denmark

autor

Buchmann K.

Bibliografia

• Bakke T.A., Harris P.D., Cable J. 2002. Host specificity dynamics: observations on gyrodactylid monogeneans. International Journal for Parasitology 32: 281-308.
• Buchmann K. 2001. Lectins in fish skin: do they play a role in monogenean – fish host interactions? Journal of Helminthology 75: 227-232.
• Buchmann K., Bresciani J. 1998. Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss: Association between mucous cell density in skin and site selection. Parasitology Research 84: 17-24.
• Buchmann K., Lindenstrøm T. 2002. Interactions between monogenean parasites and their fish hosts. International Journal for Parasitology 32: 309-319.
• Buchmann K., Uldal A. 1997. Gyrodactylus derjavini infections in four salmonids: Comparative host susceptibility and site selection of parasites. Diseases of Aquatic Organisms 28: 13-22.
• Buchmann K., Madsen K.K., Dalgaard M.B. 2004. Homing of Gyrodactylus salaris and G. derjavini (Monogenea) on different hosts and response post-attachment. Folia Parasitologica 51: 263-267.
• Hirazawa N., Oshima S., Mitsuboshi T., Yamashita S. 2003. Mucus pH of the tiger puffer Takifugu rubripes is an important factor for host identification by the monogenean Heterobothrium okamotoi. Parasitology 127: 225-230.
• Jørndrup S., Buchmann K. 2005. Carbohydrate localization on Gyrodactylus salaris and G. derjavini and corresponding carbohydrate binding capacity of their hosts Salmo salar and S. trutta. Journal of Helminthology 79: 1-6.
• Kearn G. 1967. Experiments on host-finding and hostspecificity in the monogenean skin parasite Entobdella soleae. Parasitology 57: 585-605.
• Lyons K.M. 1969. Sense organs of monogenean skin parasites ending in a typical cilium. Parasitology 59: 611-623.
• Malmberg G. 1993. Gyrodactylidae et gyrodactylose des salmonidae. Bulletin Français de la Pêche et de la Pisciculture 328: 5-46.
• Mo T.A. 1991. Seasonal variations of opisthaptoral hard parts of Gyrodactylus salaris Malmberg, 1957 (Monogenea: Gyrodactylidae) on parr of Atlantic salmon Salmo salar L. in laboratory experiments. Systematic Parasitology 20: 11-20.
• Mo T.A. 1993. Seasonal variations of opisthaptoral hard parts of Gyrodactylus derjavini Mikailov, 1975 (Monogenea: Gyrodactylidae) on brown trout Salmo trutta L. parr and of Atlantic salmon Salmo salar L. parr in the river Sandvikselva, Norway. Systematic Parasitology 26: 225-231.
• Mo T.A. 1997. Seasonal occurrence of Gyrodactylus derjavini (Monogenea) on brown trout, Salmo trutta, and Atlantic salmon, S. salar, in the Sandvikselva river, Norway. Journal of Parasitology 83: 1025-1029.
• Watson N.A., Rohde K. 1994. Two new sensory receptors in Gyrodactylus sp. (Platyhelminthes, Monogenea, Monopisthocotylea). Parasitology Research 80: 442-445.
• Whittington I.D., Cribb B.W., Hamwood T.E., Halliday J.A. 2000. Host specificity of monogenean (platyhelminth) parasites: a role for anterior adhesive areas? International Journal for Parasitology 30: 305-320.
• Yoshinaga T., Nagakura T., Ogawa K., Fukuda Y., Wakabayashi H. 2002. Attachment-inducing capacities of fish skin epithelial extracts on oncomiracidia of Benedenia seriolae (Monogenea: Capsalidae). International Journal for Parasitology 32: 381-384.
• Yoshinaga T., Nagakura T., Ogawa K., Wakabayashi H. 2000. Attachment-inducing capacities of fish tissue extract on oncomiracidia of Neobenedenia girellae (Monogenea, Capsalidae). Journal of Parasitology 86: 214-219.