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2002 | 48 | 3 |

Tytuł artykułu

Wystepowanie DNA Pneumocystis carinii i przeciwcial pneumocystozowych w surowicach niemowlat w okresie fizjologicznego spadku odpornosci

Treść / Zawartość

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
Occurrence of Pneumocystis carinii DNA aod anti-Pneumocystis antibodies in sera of infants at the age of humoral response natural degradation. The polymerase chain reaction (PCR) and indirect immunofluorescent test (IF) were used for examination of serum samples obtained from infants with respiratory tract infections. Sixty (11,9%) out of the 503 examined infant samples were positive for anti-P. carinii IgM and 354 (70,4%) contained anti-Pneumocystis IgG. P. carinii DNA was found in 6 (6,7%) sera from 90 of infected infants. Five out these 6 samples were for anti-Pneumocystis antibodies positive; 4 contained both IgG and IgM classes and one bad only IgG. The sixth sample had neither IgG nor IgM, despite of P. carinii DNA presence. The results of the studies indicated that for diagnosis Pneumocystis carinii pneumonia (PCP) in infants on serum specimens detection of antibodies by IF test is of greater value than Pneumocystis DNA amplification by PCR method.

Wydawca

-

Rocznik

Tom

48

Numer

3

Opis fizyczny

s.287-292,tab.,bibliogr.

Twórcy

autor
  • Panstwowy Zaklad Higieny, ul.Chocimska 24, 00-791 Warszawa
autor

Bibliografia

  • Arvin A.M., Yeager A.S. 1990. Protozoan and helminth infections (including Pneumocystis carinii). In: Infectious diseases of the fetus and newborn infant (Eds. J .S. Remington and J. O . Klein) W.B. Saunders Co., Philadelphia, 528-573.
  • Atzori C., Jang-Jih L., Jiang B., Bartlett M.S., Orlando G., Queener S.F., Smith J.W., Cargnel A., Lee Ch. 1995. Diagnosis of Pneumocystis carinii pneumonia in AIDS patients by using polymerase chain reactions on serum specimens. Journal of Infectious Disease 172: 1623-1626.
  • Burke B.A., Good R.A. 1972. Pneumocystis carinii infection. Medicine 52: 23.
  • Gołąb E., Sobolewska A., Bitkowska E., Bednarska A., Berak H., Dzbeński T.H., Horban A. 2001. .,Nested" PCR w diagnostyce przypadków pneumocystozowego zapalenia pluc (PCP) u pacjentów z AIDS. Wiadomości Parazytologiczne 47: 521-525.
  • Gołąb E., Sobolewska A., Bitkowska E., Bednarska A., Berak H., Dzbeński T .H. 2002. Detection of serum Pneumocystis carinii DNA in the course of P. carinii pneumonia in experimental animals and HIV-infected patients. Acta Parasitologica 47: (w druku).
  • Lipschik G.Y., Gill V.J., Lundgren J.D., Andrawis V.A., Nelson NA, Nielsen I.O., Ognibene F.P., Kovacs J.A. 1992. Improved diagnosis of Pneumocystis carinii by polymerase chain reaction on induced sputum and blood. Lancet 340: 203-206.
  • Meyers J.D., Pifer L.L., Sale G.E., Thomas E.D. 1979. The value of Pneumocystis carinii antibody and antigen detection for diagnosis of Pneumocystis carinii pneumonia after marrow transplantation. American Review of Respiratory Disease 120: 1283-1287.
  • Nowosławski A., Brzosko W. 1964. Indirect immuoofluorescent test for serodiagnosis of Pneumocystis carinii infection. Bulletin de L'Academie Polonaise des Science 12: 143-147.
  • Pifer L.L, Hughes W.T., Stagno S., Woods D. 1978. 41 Pneumocystis carinii infection: evidence for high prevalence in normal and immunosuppressed children. Pediatrics 61: 35-41.
  • Rabodonirina M., Raffenot D., Cotte L., Boibieux A., Mayencon, M., Bayle G., Persat F., Rabatel F., Trepo C., Peyramond D., Piens M-A. 1997. Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from Human Immunodeliciency Virus-infected patients: use of a simple DNA extraction procedure and nested PCR. Journal of Clinical Microbiology 35: 2748-2751.
  • Richards C.G.M., Wakefield A.E., Mitchell C.D. 1994. Detection of Pneumocystis carinii DNA in nasopharyngeal aspirates of leukaemic infants with pneumonia. Archives of Disease in Childhood 71: 254-255.
  • Schluger N., Godwin T., Sepkowitz K., Armstrong O., Bernard E., Rifkin M., Cerami A, Bucala R. 1992. Application of DNA amplification to pneumocystosis: presence of serum Pneumocystis carinii DNA during human and experimentally induced Pneumocystis carinii pneumonia. Journal of Experimental Medicine 176: 1327-1333.
  • Sobolewska A, Dzbeński T.H. 1997. Epidemiologia i diagnostyka laboratoryjna inwazji powodowanych przez Pneumocystis carinii. Przegląd Epidemiologiczny 51: 1-2.
  • Tamburrini E, Mencarini P, Visconti E., Zolfo M, De Luca A., Siracusaoo A., Ortona E., Wakefield A.E. 1996. Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia. Journal of Clinical Microbiology 34: 1586-1588.
  • Wakefield A.E., Pixley F.J., Banerji S., Sinclair K., Miller R.F., Moxon E.R., Hopkin J.M. 1990. Amplification of mitochondrial ribosomal RNA sequences for Pneumocystis carinii DNA of rat and human origin. Molecular and Biochemical Parasitology 43: 69-76.
  • Wakefield A.E., Guiver L, Miller R.F., Hopkin J.M. 1991. DNA amplificatin on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet 337: 1378-79.
  • Walzer P .D., Perl D.P., Krogstad D.J., Rawson P.G, Schultz M.G. 1974. Pneumocystis carinii penumonia in the United States. Epidemiologic, diagnostic, and clinical features. Annals of Internal Medicine 80: 83-93.

Typ dokumentu

Bibliografia

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Identyfikator YADDA

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