Cloning of the fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. IX. The effects of the cloning experiments

• Autorzy: Borowicz B P
• Języki publikacji: EN

Abstrakty

EN

The goal of these experiments was to clone the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans into the pET-3a vector to translate them in the T7 RNA polymerase / promoter system in BL21(DE3) cells of E. coli. This goal has been achieved and the results of the cloning experiments are presented and discussed.

PL

Celem wykonanych eksperymentów było wklonowanie fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans, do wektora pET-3a w celu ich translacji w systemie promotora T7 RNA polimerazy w komórkach E. coli szczepu BL21(DE3). Cel ten został zrealizowany, a wyniki eksperymentów klonowania przedstawiono i omówiono.

Słowa kluczowe

EN

pET-3a vector; promoter system; cloning; I-18 C gene; T7 RNA polymerase; Chironomus tentans; polymerase chain reaction; cloning experiment

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 1999
• Tom: 39
• Numer: 1
• Opis fizyczny: p.80-90,fig.

Twórcy

autor

Borowicz B P

• Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland

Bibliografia

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