Detection of bovine leukaemia virus proviral DNA in paraffin sections, tissue smears, and peripheral blood leucocytes by in situ PCR

• Autorzy: Kubis P , Szczotka M. , Kuzmak J.
• Języki publikacji: EN

Abstrakty

EN

In situ PCR was developed for the detection of proviral DNA of bovine leukaemia virus in paraffin embedded tissues, tissue smears of experimentally inoculated sheep, as well in peripheral blood leukocytes of naturally infected cattle. The liver, spleen, lung, heart, kidney, and lymph node were collected from two infected sheep, which died or were euthanized due to lymphosarcoma and persistent lymphocytosis, respectively. Peripheral blood leukocytes (PBLs) were isolated from eleven cattle serologically positive for BLV. The presence of proviral DNA was confirmed in all paraffin embedded tissue sections and tissue smears as well in all PBLs preparations. In situ PCR results agreed 100% with those of provirus detection using conventional PCR. Newly developed in situ PCR would be recommended as a method to differentiate between EBL and other lymphosarcomas of unknown aetiology.

Słowa kluczowe

EN

peripheral blood; in situ; tissue smear; veterinary medicine; cattle; paraffin section; bovine leukemia virus; polymerase chain reaction; detection; leucocyte; animal disease

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2007
• Tom: 51
• Numer: 3
• Opis fizyczny: p.337-342,fig.,ref.

Twórcy

autor

Kubis P

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Szczotka M.

autor

Kuzmak J.

Bibliografia

• 1. Ballagi-Pordany A., Klintevall K., Merza M., Klingeborn B., Belak S.: Direct detection of bovine leukemia virus infection: practical applicability of a double polymerase chain reaction. J Vet Med B 1992, 39, 69-77.
• 2. Buehring G.C., Kramme P.M., Schultz R.D.: Evidence for bovine leukemia virus in the mammary epithelial cells of infected cow. Lab Invest 1994, 71, 359-365.
• 3. Burny A., Cleuter Y., Kettmann R., Mammerickx M., Marbaix G., Portetelle D., Van Den Broeke A., Willems L., Thomas R.: Bovine leukaemia: facts and hypotheses derived from the study of an infectious cancer. Vet Microbiol 1988, 17, 197-218.
• 4. Cockerell G.L., Rovnak J.: The correlation between the direct and indirect detection of bovine leukemia virus infection in cattle. Leuk Res 1988, 12, 465-469.
• 5. Duncan R.B., Scarratt W.K., Buehring G.C.: Detection of bovine leukemia virus by in situ polymerase chain reaction in tissues from a heifer diagnosed with sporadic thymic lymphosarcoma. J Vet Diagn Invest 2005, 17, 190-194.
• 6. Eaves F.W., Molloy J.B., Dimmock C.K., Eaves L. E.: A field evaluation of the polymerase chain reaction procedure for the detection of bovine leukaemia virus proviral DNA in cattle. Vet Microbiol 1994, 39, 313-321.
• 7. Kettmann R., Burny A., Cleuter Y., Ghysdael J., Mammerickx M.: Distribution of bovine leukemia virus proviral sequences in tissues of bovine, ovine and human origin. Ann Rech Vet 1978, 9, 837-844.
• 8. Klintevall K., Ballagi-Pordany A., Naslund K., Belak S.: Bovine leukaemia virus: rapid detection of proviral DNA by nested PCR in blood and organs of experimentally infected calves. Vet Microbiol 1994, 42, 191-204.
• 9. Levraud J.P.: Some new techniques for RNA or DNA analysis of interest for immunologists. Res Immunol 1998, 149, 119-125.
• 10. Mirsky M.L., Olmstead C.A., Da Y., Lewin H.A.: The prevalence of proviral bovine leukemia virus in peripheral blood mononuclear cells at two subclinical stages of infection. J Virol 1996, 70, 2178-2183.
• 11. Naif H.M., Brandon R.B., Daniel R.C.W., Lavin M.F.: Bovine leukaemia proviral DNA detection in cattle using the polymerase chain reaction. Vet Microbiol 1990, 25, 117-129.
• 12. Naif H.M., Daniel R.C.W., Cougle W.G., Lavin M.F.: Early detection of bovine leukemia virus by using an enzyme-linked assay for polymerase chain reaction amplified proviral DNA in experimentally infected cattle. J Clin Microbiol 1992, 30, 675-679.
• 13. Reichert M., Stec J.: Simultaneous use of two primer pairs increases the efficiency of polymerase chain reaction assay in the diagnosis of bovine leukemia virus infection. J Vet Diagn Invest 1999, 11, 543-547.
• 14. Rovnak J., Casey J.W., Boyd A.L., Gonda M.A., Cockerell G.L.: Isolation of bovine leukemia virus infected endothelial cells from cattle with persistent lymphocytosis. Lab Invest 1991, 65, 192-202.
• 15. Schwartz I., Levy D.: Pathobiology of bovine leukemia virus. Vet Res 1994, 25, 521-536.
• 16. Szczotka M., Buzała E., Dereń W., Kubiś P.: Flow cytometry determination of Bcl-2 expression in animals infected with bovine leukaemia virus. Bull Vet Inst Pulawy 2006, 50, 9-14.
• 17. Taylor R.G., Logan P.: The polymerase chain reaction: new variations on an old theme. Curr Opin Biotechnol 1995, 6, 24-29.
• 18. Van Der Maaten M.J., Miller J.M.: Bovine Leukosis Virus. In: Virus Infection of Ruminants, edited by Z. Dinter and B. Morein (Amsterdam: Elsevier Science B. V.) 1990, pp. 419-429.
• 19. Willems L., Kettmann R.: Deltaretrovirus. Retroviridae. In: The Springer Index of Viruses, edited by C. A. Tidona and G. Darai (Berlin Heidelberg New York: Springer- Verlag) 2001, pp. 1014-1019.
• 20. Xie B., Oyamada T., Yoshikawa H., Oyamada T., Yoshikawa T.: Detection of proviral DNA of bovine leukaemia virus in cattle by a combination of in-situ hybridization and the polymerase chain reaction. J Comp Path 1997, 116, 87-96.
• 21. Zhang S., Troyer D. L., Kapil S., Zheng L., Kennedy G., Weiss M., Xue W., Wood C., Minocha H.C.: Detection of proviral DNA of bovine immunodeficiency virus in bovine tissues by polymerase chain reaction (PCR) and in situ hybridization. Virology 1997, 236, 249-257.