An isotopic assay of dUTPase activity based on coupling with thymidylate synthase

• Autorzy: Golos B. , Rode W.
• Języki publikacji: EN

Abstrakty

EN

A new rapid, sensitive and convenient procedure is presented allowing determination of dUTPase activity. With [5-3H]dUTP used as the substrate, dUTPase, converts it to the corresponding monophosphate and is coupled with a thymidylate synthase-catalyzed reaction, resulting in tritium release from [5-3H]dUMP. Following charcoal absorption of the labeled nuleotides, radioactivity of tritiated water is determined. The new assay was tested to show comparable results with a previously described assay, based on measuring dUTPase-catalyzed [5-3H]dUMP production.

Słowa kluczowe

EN

dUTPase activity; thymidylate synthase; coupling; monophosphate

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 3
• Opis fizyczny: p.837-840,fig.

Twórcy

autor

Golos B.

• Polish Academy of Sciences, L.Pasteura 3, 02-093 Warsaw, Poland

autor

Rode W.

Bibliografia

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