The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of bovine erythrocytes

• Autorzy: Wrobel A , Lukaszynska B. , Kedzierska J.
• Języki publikacji: EN

Abstrakty

EN

Bovine erythrocytes treated with peroxynitrite (ONOO-), a cytotoxic species formed in vivo via the reaction of nitric oxide (NO*) and the superoxide anion (O2-*), show an increased rate of hemolysis on sudden osmotic stress. The increase in the rate was peroxynitrite concentration dependent. In the presence of some antioxidants (uric acid, ascorbic acid, glutathione, melatonin and albumin), this effect was significantly lower, with ascorbic acid as the most efficient antioxidant.

Słowa kluczowe

EN

osmotic hemolysis; nitric oxide; peroxynitrite; erythrocyte; stopped-flow technique; antioxidant; hemolysis; bovine erythrocyte; superoxide anion

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 2
• Opis fizyczny: p.455-460,fig.

Twórcy

autor

Wrobel A

• Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland

autor

Lukaszynska B.

autor

Kedzierska J.

Bibliografia

• 1.Radi, R., Beckman, J.S., Bush, K.M. and Freeman, B.A. Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch. Biochem. Biophys. 288 (1991) 481-487.
• 2.Alvarez, B., Ferrer-Sueta, G., Freeman, B.A. and Radi, R. Kinetics of peroxynitrite reaction with amino acids and human serum albumin. J. Biol. Chem. 274 (1999) 842-848.
• 3.Quijano, C., Alvarez, B., Gatti, R.M., Augusto, O. and Radi, R. Pathways of peroxynitrite oxidation of thiols groups. Biochem. J. 322 (1997) 167-173.
• 4.Soszyński, M. and Bartosz, G. Effects of peroxynitrite on erythrocytes. Biochim. Biophys. Acta 1291 (1996) 107-114.
• 5.Uppu, R. M., Squadrito, G. L., Cueto R, and Pryor, W. A. Synthesis of peroxynitrite by azide-ozone reaction. Meth. Enzymol. 269 (1996) 311- 321.
• 6.Araki, K. and Rifkind, J.M. The rate of osmotic hemolysis. A relationship with membrane bilayer fluidity. Biochim. Biophys. Acta 645 (1981) 81-90.
• 7.Dodge, J.T., Mitchell, C. and Hanahan, D.J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocyte. Arch, Biochem. Biophys. 100 (1963) 119-130.
• 8.Gordon, L.M. and Curtain, C.C. Electron spin resonance analysis of model and biological membranes. In: Methods for studying membrane fluidity, (Aloia, R.C., Curtain, C.C and Gordon, L.M., Eds.), Alan R. Liss, Inc., N.Y.,1988, 25-88.
• 9.Arteel, G.E., Briviba, K. and Sies, H. Protection against peroxynitrite. FEBS Lett. 445 (1999) 226-230.
• 10.Mosior, M., Bobrowska, M. and Gomułkiewicz, J. Effect of the level of ATP and of the state of spectrin on osmotic properties of bovine erythrocytes. Biochim. Biophys. Acta 1022 (1990) 355-360.