Ocena przydatnosci wybranych systemow komercyjnych do identyfikowania ziarenkowcow z rodzaju Streptococcus

• Autorzy: Fordymacki P. , Kochman M. , Lawrynowicz-Paciorek M. , Kaluzewski S.
• Języki publikacji: PL

Abstrakty

PL

Porównano trzy komercyjne zestawy do identyfikacji paciorkowców. Najwyższy odsetek prawidłowo zidentyfikowanych szczepów paciorkowcowych uzyskano w systemie rapid ID 32 STREP - 80,9%. W systemie API 20 STREP odsetek ten wynosił 76,4%, a w systemie PPL 18 - 61,8%. Żaden z ocenianych komercyjnych systemów nie zapewnia wiarygodnej identyfikacji paciorkowców z serologicznej grupy G. Systemy API 20 STREP i Streptoplast PPL 18 są nieprzydatne do identyfikowania szczepów S. pneumoniae.

EN

The usefulness was assessed of three commercially available systems for rapid identification of streptococcal strains. The studied material comprised 68 strains of streptococci and enterococci (including 24 standard strains) belonging to serological groups: A (14 strains), B (10), C (11), D (10), F (3) and G (10), as well as 10 S. pneumoniae strains. The strains were isolated from throat, nasal, wound swabs, blood, pus of inpatients and throat and nasal swabs of outpatients. For the identification of streptococci 3 commercially available systems were used: API 20 STREP (bioMerieux, France), rapid ID 32 Strep (bioMerieux, France), Streptoplast PPL 18 (HTL, Poland). The determinations were done according to producer's instructions. The highest percent of correctly identified strains was obtained with the rapid ID 32 Strep - 80,9%, with the API 20 STREP - 76,4% strains were identified correctly and with the PPL 18 - 61,8%. The study showed that the API 20 STREP and rapid ID 32 STREP are suitable for the identification of streptococcal strains from groups: A, B, C, D, F and enterococci - group D. The proportions of correctly identified strains from these groups with the Streptoplast PPL 18 were lower than those determined with the bioMerieux systems. Using of three identification systems streptococci from group G and S. pneumoniae strains cannot be identified.

Słowa kluczowe

PL

system rapid ID 32 STREP; paciorkowce; ziarenkowce; system API 20 STREP; systemy komercyjne; Streptococcus; bakterie; identyfikacja

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1998
• Tom: 50
• Numer: 3-4
• Opis fizyczny: s.171-177,rys.,tab.,bibliogr.

Twórcy

autor

Fordymacki P.

• Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24

autor

Kochman M.

autor

Lawrynowicz-Paciorek M.

autor

Kaluzewski S.

Bibliografia

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