Clonal multiplication of Lithospermum canescens [Michx.] Lehm. and Onosma paniculatum [Bur. and Franch]

• Autorzy: Syklowska-Baranek K , Pietrosiuk A. , Dluska H. , Furmanowa M.
• Języki publikacji: EN

Abstrakty

EN

An efficient plant micropropagation system was established using shoot tips and stem segments of Lithospermum canescens and Onosma paniculatum (Boraginaceae). A high frequency of axillary shoot formation was achieved on LS [15] medium with 0.5 mg/1 kinetin and 2.0 mg/1 BAP (average shoot formation per explant was 6.18 ± 1.89 for L. canescens and 3.01 ± 0.82 for 0. paniculatum). The best results were obtained with 1x8 clone of L canescens. Axillary branching and root formation were the highest (8.22 shoot formation, 46.7% of rooting). Contamination of the explants with Rhodococcus fascians D188 increased the number of newly formed shoots of 0. paniculatum to 4.6 ± 0.72, while the propagation process of L. canescens was not affected. The highest percentage of rooted shoots of L canescens was observed on V, B5 medium [14] with 0.2 mg/1 1BA (from 27% to 46.7%, depending on plant line), while in the case of regenerated shoots of 0. paniculatum hormone-free LS medium induced roots more effectively (36.7%) than V2 B5 medium with 0.2 mg/1 1BA (25%).

PL

Wydajny sposób mikrorozmnażania roślin Lithospermum canescens (Michx.) Lehm, i Onosma paniculatum (Bur. and Franch) (Boraginaceae) opracowano z wykorzystaniem pąków szczytowych oraz bocznych. Największą liczbę pędów rozwijających się z eksplantatów obserwowano na pożywce LS (15] z kinetyną (0,5 mg/1) i BAP (2,0 mg/l). Średnia liczba rozwijających się pędów w wypadku L canescens wyniosła 6,18 ± 1,89, a w wypadku 0. paniculatum 3,01 ± 0,82. Najlepsze wyniki uzyskano dla klonu Lc8 L. canescens. Liczba uzyskanych pędów z istniejących merystemów wyniosła 8,22, a procent ukorzenionych pędów - 46,7. Zakażenie eksplantatów roślinnych szczepem bakteryjnym Rhodococcus fascians Dl 88 zwiększało liczbę nowopowstających pąków w kulturze 0. paniculatum do 4,6 ± 0,72, natomiast w wypadku L. canescens proces ten nie byt efektywny. Najwyższy procent ukorzenionych pędów L canescens otrzymano na pożywce V. B5 (141-7~IBA (zależnie od linii: od 27% do 46,7%), podczas gdy wytwarzanie korzeni 0. paniculatum następowało lepiej (36,7%) na pożywce LS bez regulatorów wzrostu.

Słowa kluczowe

EN

Onosma paniculatum; Lithospermum canescens; Boraginaceae; micropropagation; multiplication; clonal multiplication

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2004
• Tom: 50
• Numer: 2
• Opis fizyczny: p.55-64,fig.,ref.

Twórcy

autor

Syklowska-Baranek K

• Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Pietrosiuk A.

autor

Dluska H.

autor

Furmanowa M.

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