Prostaglandin E2 (PGE2) and thromboxane A2 (TXA2) synthesis is regulated by conjugated linoleic acids (CLA) in human macrophages

• Autorzy: Stachowska E. , Dolegowska B. , Dziedziejko V. , Rybicka M. , Kaczmarczyk M. , Bober J. , Rac M. , Machalinski B. , Chlubek D.
• Języki publikacji: EN

Abstrakty

EN

Conjugated linoleic acid (CLAs) are positional and geometric isomers of linoleic acid with have a potential anti-atherosclerotic and anti-inflammation properties. Metabolites of arachidonic acid - prostaglandins and thromboxans are endogenous mediators of inflammation. Prostaglandin E2 and thromboxan A2 which are a products of two izoformes of cyclooxygenases (COX-1 and COX-2) in macrophages, play an important role in this process. COX - 1 is a constitutive enzyme, whereas the COX - 2 is inducible and its amount in the cell rapidly increases during inflammation (e.g. via NF B pathway). The aim of the study was to test the effect of CLAs on cyclooxygenases (COX-1 and COX-2) activity, their mRNA expression and protein content in macrophages. Additionally the active form of the kB (NF B) transcription factor was measured. For the experiments monocytes from monocytic cell line (THP-1) and from human venous blood were used. Monocytes were differentiated to macrophages and cultured with 30 µM CLAs or linoleic acid for 48 h. The COX-1 and COX-2 products - PGE2 and TXB2, were measured by ELISA method. The enzymes (COX-s) activity were estimated by spectroscopic method. mRNA expression and protein analysis were analysed by real-time PCR and Western blot technique. In macrophages cultured with CLAs reduction of TXB2 and PGE2 concentration was observed. Significant change in COX-2 expression in cells cultured with trans-10, cis-12 CLA (in macrophages obtained from peripheral blood) was observed. COX-1 inhibition was resulting from competition of CLA and linoleic acid with arachidonic acid.

Słowa kluczowe

EN

macrophage; man; prostaglandin E2; eicosanoid; monocyte; cyclooxygenase-1; cyclooxygenase-2; synthesis; conjugated linoleic acid; thromboxane A2

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2009
• Tom: 60
• Numer: 1
• Opis fizyczny: p.77-85,fig.,ref.

Twórcy

autor

Stachowska E.

• Pomeranian Medical University, Zolnierska 48, 71-210 Szczecin, Poland

autor

Dolegowska B.

autor

Dziedziejko V.

autor

Rybicka M.

autor

Kaczmarczyk M.

autor

Bober J.

autor

Rac M.

autor

Machalinski B.

autor

Chlubek D.