Immunocytochemical identification of neoplastic cell clone in phase S of cell cycle in transplantable rat tumors

• Autorzy: Nowak M. , Madej J.A. , Dzimira S. , Cegielski M. , Calkosinski I.
• Języki publikacji: EN

Abstrakty

EN

Wistar and Buffalo rats of both sexes, aged 4 months, were divided into three groups: I which was given an intramuscular injection of 3 x 10 6 cells of Morris hepatoma (Buffalo males), II - subcutaneous injection of 3 x 10 4 cells of mammary gland carcinoma (Wistar females), III - intraperitoneal injection of 3 x 104 cells of Yoshid sarcoma (Wistar males). The animals were killed: in group I - 19, group II - 13 and in group III - 6 days after tumor transplantation. Twenty four hours before euthanasia the rats were given 5-brome-2'-deoxyuridine (BRd-U) at a dose of 50 mg/kg body mass. The control group consisted of animals with tumour. They were not treated with BRd-U. Im- munocytochemical reaction was performed on the sections of tumors, using monoclonal anti-BRd-U clone BU-33, Sigma. Computer measurements of tumor cells were carried out. There was a high similarity in morphological parameters between two kinds of cancer, and clear differences between them and Yoshid sarcoma. The main difference was noted in a twofold increase in the quantity of synthesised DNA in the nuclei of sarcoma cells. Immunocytochemical identification of tumor cells in phase S of the cell cycle with the use of monoclonal anti-BRd-U antibody is a precise and quick method of estimation of their proliferative potential.

Słowa kluczowe

EN

cell cycle; neoplastic cell clone; Yoshid sarcoma; mammary gland carcinoma; Morris hepatoma; tumour; rat; identification

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2004
• Tom: 07
• Numer: 3
• Opis fizyczny: p.163-170,fig.,ref.

Twórcy

autor

Nowak M.

• Agricultural Academy in Wroclaw, Norwida 31, 50-375 Wroclaw, Poland

autor

Madej J.A.

autor

Dzimira S.

autor

Cegielski M.

autor

Calkosinski I.

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