Desorption-ionization on silicon for small molecules: a promising alternative to MALDI TOF

• Autorzy: Kraj A. , Dylag T. , Gorecka-Drzazga A. , Bargiel S. , Dziuban J. , Silberring J.
• Języki publikacji: EN

Abstrakty

EN

A method has been developed for laser desorption/ionization of catecholamines from porous silicon. This methodology is particularly attractive for analysis of small molecules. MALDI TOF mass spectrometry, although a very sensitive technique, uti­lizes matrices that need to be mixed with the sample prior to their analysis. Each ma­trix produces its own background, particularly in the low-molecular mass region. Therefore, detection and identification of molecules below 400 Da can be difficult. Desorption/ionization of samples deposited on porous silicon does not require addi­tion of a matrix, thus, spectra in the low-molecular mass region can be clearly read­able. Here, we describe a method for the analysis of catecholamines. While MALDI TOF is superior for proteomics/peptidomics, desorption/ionization from porous sili­con can extend the operating range of a mass spectrometer for studies on metabo- lomics (small organic molecules and their metabolites, such as chemical neuro­transmitters, prostaglandins, steroids, etc.).

Słowa kluczowe

EN

desorption; silicon; small molecule; peptide; mass spectrometry; catecholamine; porous silicon; ionization; matrix-assisted laser desorption

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 3
• Opis fizyczny: 783-787,fig.

Twórcy

autor

Kraj A.

• Jagiellonian University, R.Ingardena 3, 30-060 Krakow, Poland

autor

Dylag T.

autor

Gorecka-Drzazga A.

autor

Bargiel S.

autor

Dziuban J.

autor

Silberring J.

Bibliografia

• Andersen JS, Svensson B, Roepstorff P. (1996) Electrospray ionization and matrix assisted laser desorption/ionization mass spectrometry: powerful analytical tools in recombinant protein chemistry. Nat Biotechnol.; 14: 449-57.
• Bergquist J, Sciubisz A, Kaczor A, Silberring J. (2002) Catecholamines and methods for their identification and quantitation in biological tissues and fluids. J Neurosci Methods.; 113: 1-13.
• Kowalski P, Stoerker J. (2000) Accelerating discoveries in the proteome and genome with MALDI TOF MS. Pharmacogenomics.; 1: 359-66.
• Mann M, Hendrickson RC, Pandey A. (2001) Analysis of proteins and proteomes by mass spectrometry. Annu Rev Biochem.; 70: 437-73.
• Nieradko L. (2001) Mikroelektroniczne metody modyfikacji wlasciwosci separujacych mikromechanicznych kapilarnych kolumn chromatograficznych. Ph.D. Thesis, Wroclaw University of Technology, Department of Electronics (in Polish).
• Shen Z, Thomas JJ, Averbuj C, Broo KM, Engelhard M, Crowell JE, Finn M.G, Siuzdak G. (2001) Porous silicon as a versatile platform for laser desorption/ionization mass spectrometry. Anal Chem.; 73: 612-9.
• Thomas JJ, Shen Z, Crowell JE, Finn MG, Siuzdak G. (2001) Desorption/ionization on silicon (DIOS): a diverse mass spectrometry platform for protein characterization. Proc Natl Acad Sci US A.; 98: 4932-7.
• Wei J, Buriak JM, Siuzdak G. (1999) Desorption-ionization mass spectrometry on porous silicon. Nature.; 399: 243-6.