Usefulness of strb1 and 16S rDNA-targeted PCR for detection of Streptomyces spp. in environmental samples

• Autorzy: Saadoun I , Gharaibeh R.
• Języki publikacji: EN

Abstrakty

EN

In this study, we revealed rapid detection of streptomycin-producing Streptomyces spp. by extraction of total soil DNA from 14 soil samples using a modified lysis method followed by PCR amplification of a genus-specific sequence in the Streptomyces' 16S rDNA gene DNA band of the expected size (438 bp) was seen with all the samples. Additionally, specific amplification of the streptomycin-coding gene (strbl) directly from soil revealed the presence of a single DNA band of 940 bp. These results indicate that PCR-amplification of Streptomyces specific genes could be used for direct detection of streptomycin-producing Streptomyces species from soil.

Słowa kluczowe

EN

polymerase chain reaction; identification; detection; streptomycin-coding gene; 16S rDNA gene; environmental sample; soil sample; Streptomyces

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 1
• Opis fizyczny: p.81-84,fig.,ref.

Twórcy

autor

Saadoun I

• Jordan University of Science and Technology, P.O.Box 3030, Irbid-22110, Jordan

autor

Gharaibeh R.

Bibliografia

• Distler J., K. Mansouri, G. Mayer, W. Stockmann and W. Piepersberg. 1992. Streptomycin biosynthesis and its regulation in streptomycetes. Gene 115: 105-111.
• EL-Tarabily K. A., G.E.ST. J., Hardy, K. Sivasithamparam, A.M. Hussein and D.E Kurtboke. 1997. The potential for the biological control of cavity-spot disease of carrots, caused by Pythium coloratum, by streptomycete and non-streptomycete actinomycetes. New Phytol. 137: 495-507.
• Gharaibeh R., I. Saadoun and A. Mahasneh. 2003. Evaluation of combined 16S rDNA and strb 1 gene targeted PCR to identify and detect streptomycin-producing Streptomyces. J. Basic Microbiol. 4: 301-311.
• Hain T., N. Ward-Rainey, M. Kroppenstedt, E. Stackebrandt and F. Rainey. 1997. Discrimination of Streptomyces albidoflavus strains based on the size and number of 16S-23S ribosomal DNA intergenic spacers. Inter. J. System. Bacteriol. 47: 202-206.
• Huddleston A.S., N Cresswell, M.C.P. Neves, J.E. Beringer., S. Baumberg, D.L.Thomas and E.M.H. Wellington. 1997. Molecular detection of streptomycin-producing streptomycetes in Brazilian soils. Appl. Environ. Microbiol. 63: 1288-1297.
• Mehling A., U.F. Wehmeier and W. Piepersberg. 1995. Application of random amplified polymorphic DNA (RAPD) assays in identifying conserved regions of actinomycete genomes. FEMS Microbiol. Lett. 128: 119-126.
• Ogram A., G.S. Sayler and T. J. Barkay. 1987. Extraction and purification of microbial DNA from sediments. J. Microbiol. Meth. 7: 57-66.
• Retzlaff L., G. Mayer, S. Beyer, J. Ahlert, S. Verseck, J. Distler and W. Piepersberg. 1993. Streptomycin production in streto-mycetes: A progressive report. Pp. 183-194. In: Blatz R., G.D. Hegeman and PL. Skatrud (eds). Industrial Microorganisms: Basic and Molecular Genetics. ASM Press, Washington, D.C.
• Saadoun I. and F. AL-Momani. 1997. Steptomycetes from Jordan soils active against Agrobacteriutn tumefaciens. Actinomycetes 8: 29-36.
• Saadoun I. F. Al-Momani, H. Malkawi and M.J. Mohammad. 1999. Isolation, identification and analysis of antibacterial activity of soil streptomycetes isolates from North Jordan. Microbios 100: 41-46.
• Saadoun I., K.M. Hameed, F. AL-Momani, H. Malkawi, M. Meqdam and M.J. Mohammad. 2000. Characterization and analysis of antifungal activity of soil streptomycetes isolated from North Jordan. Egypt. J. Microbiol. 35: 463-471.
• Saadoun I. and R. Gharaibeh. 2002. The Streptomyces flora of Jordan and its' potential as a source of antibiotics active against antibiotic-resistant Gram-negative bacteria. World J. Microbiol. Biotech. 18: 465-470.
• Sambrook J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: a Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
• Shirling E.B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16: 313-340.
• Tahtamouni M.E.W., K. M. Hameed and I. Saadoun. I. 2006. Biological control of Sclerotinia sclerotiorum using indigenous chitinolytic actinomyctes in Jordan. Plant Pathol. J. 22: 107-114.
• TanakaY. and S. Omura. 1990. Metabolism and products of actinomycetes - An introduction. Actinomycetologica 4: 13-14.
• Torsvik V., J.P. Goksoyr and F.L. Klein. 1990. High diversity in DNA of soil bacteria. Appl. Environ. Microbiol. 56: 782-787.
• Tulemisova E. and T. Nikitina. 1989. Search for actinomycetes antagonists of fungi causing sugar beet root rot. Acta Biotechnol. 9: 389-391.
• Weller D.M. and S.L. Thomashow. 1990. Antibiotics: evidence for their production and sites where they are produced, pp. 703-711. In: , Barker R.R., P.E. Dunn and R. Alan (eds). New Directions in Biological Control: Alteration for Suppressing Agricultural Pests and Diseases. Liss. Inc., New York.
• Wellington E.M.H, N. Cresswell and P.R. Herron. 1992. Gene transfer between stretomycetes in soil. Appl. Environ. Microbiol. 115: 193-198.
• Williams S.T., M. Goodfellow, G. Alderson, E.M. Wellington, P.H. Sneath and M.J. Sackin. 1983. Numerical classification of Streptomyces and related genera. J. Gen. Microbiol. 129: 1743-1813.
• Williams S.T., M. Goodfellow and G. Alderson. 1989. In: Bergey's Manual of Systematic Bacteriology. Williams, S.T., M.E. Sharpe and J.G. Holt, J. G. vol. 4, pp. 2452-2508.