Genotypes of Listeria monocytogenes strains isolated from 2000 to 2002 in Poland

• Autorzy: Paciorek J , Jacquet C. , Salcedo C. , Doumith M. , Vazquez J.A. , Martin P.
• Języki publikacji: EN

Abstrakty

EN

Pulsed field gel electrophoresis (PFGE), multiplex PCR and multilocus sequence typing (MLST) methods were used for genotyping study of seventy-three L. monocytogenes isolates collected in Poland between 2000 and 2002 from human, food, environment and a diseased goat. The multiplex PCR, which is an alternative method to classical serotyping, divided the isolates into four PCR groups, IIa (42.5%), IIb (27.4%), IIc (4.1%) and IVb (26%). The isolates displayed 33 distinct PFGE profiles. Twenty eight strains were further characterised by MLST based on sequence analyses of seven housekeeping genes. The combined sequence analyses revealed a total of 10 different allelic profiles from which 3 were not described earlier. It is intended that results obtained in this study will be the first data of a national database of L. monocytogenes genotypes in Poland.

Słowa kluczowe

EN

Listeria monocytogenes; Poland; environment; consumption; food; microbiology; diseased goat; contaminated food; multilocus sequence typing; pulsed-field gel electrophoresis; human disease; isolation; genotype; human nutrition; polymerase chain reaction; method

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2006
• Tom: 55
• Numer: 1
• Opis fizyczny: p.31-35,ref.

Twórcy

autor

Paciorek J

• Voivodship Sanitary-Epidemiological Station in Warsaw, 79 Zelazna St., 00-875 Warsaw, Poland

autor

Jacquet C.

autor

Salcedo C.

autor

Doumith M.

autor

Vazquez J.A.

autor

Martin P.

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