The use immobilized methylchymotrypsin for the purification of human and sheep alpha-1-proteinase inhibitor [alpha1-PI]

• Autorzy: Grybel J , Wilusz T.
• Języki publikacji: EN

Abstrakty

EN

α1-proteinase inhibitor was isolated from albumin fractions of human and sheep plasma using a new method of purification using affinity chromatography on immobilized methylchymotrypsin in the presence of 5 M NaCl. The inhibitor was finally polished to homogenity either by chromatography on a Mono Q or a Sephacryl S-200 HR column. The presented method makes it possible to recover α1-proteinase inhibitor which has been added to cow milk.

Słowa kluczowe

EN

affinity chromatography; alpha-1-proteinase inhibitor; sheep; man; plasma; human plasma; purification; methylchymotrypsin; milk; albumin fraction

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 2
• Opis fizyczny: p.363-374,fig.

Twórcy

autor

Grybel J

• University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland

autor

Wilusz T.

Bibliografia

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