Effect of fructans with different degrees of polymerization on bacterial enzymes activity, lipid profile and antioxidant status in rats

• Autorzy: Zary-Sikorska E. , Juskiewicz J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to compare the effect of two types of fructans characterised by a different degree of polymerization (long-chain inulin and short-chain fructooligosaccharides [FOS]), added as single dietary components on the activity of selected bacterial enzymes in the faeces and the caecal digesta, the antioxidant status and the serum lipid profile in laboratory rats. Twenty four male Wistar rats were randomly divided into three groups and for 4 weeks were fed a control casein and two experimental diets supplemented with fructooligosaccharides and inulin at a level of 7.5% of diet (C, FOS and IN groups, respectively). The inulin addition caused a significant decrease in faecal pH value throughout the study (pH measurements were taken after day 7, 14, 21 and 28 of feeding), whereas the FOS-diet significantly decreased pH of fresh faeces only after 3 weeks of the study (p<0.05 vs. the control). Both experimental diets beneficially diminished the activity of faecal β-glucosidase and β-glucuronidase as compared to the control animals. The decreased activity of bacterial β-glucosidase and increased activity of β-galactosidase in the caecal digesta was noted only in the case of dietary FOS. The lowest concentrations of TBARS in the liver, kidneys and serum were observed in the IN group (p<0.05 vs. the control), however the FOS diet also effectively reduced concentration of TBARS in the liver tissue and the serum. The dietary inulin significantly decreased the serum glucose level and increased the percentage of HDL-cholesterol in total cholesterol (vs. C and FOS groups). The results obtained demonstrate that the consumption of diet supplemented with inulin seems to be more health promoting and elicits more beneficial changes in microflora activity, lipid metabolism and antioxidant status in rats than that enriched with short-chain fructooligosaccharides.

Słowa kluczowe

EN

dietary polyphenol; antioxidant status; civilization disease; obesity; human nutrition; fructan; polymerization degree; polysaccharide; rat; bacterial enzyme activity; lipid profile; laboratory animal

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2008
• Tom: 58
• Numer: 2
• Opis fizyczny: p.269-272,ref.

Twórcy

autor

Zary-Sikorska E.

• Polish Academy of Sciences, Tuwima 10 Str., 10-747 Olsztyn, Poland

autor

Juskiewicz J.

Bibliografia

• 1. Bouhnik Y., Flourie B., Riottot M., Effects of fructo-oligosaccharides ingestion on faecal bifidobacteria and selected metabolic indexes of colon carcinogenesis in healthy humans. Nutr. Cancer, 1996, 26, 21–29.
• 2. Carabin I.G., Flamm W.G., Evaluation of safety of inulin and oligofructose as dietary fiber. Regul. Toxicol. Pharm., 1999, 30, 268–282.
• 3. Cummings J.H., Macfarlane G.T., Englyst H.N., Prebiotic digestion and fermentation. Am. J. Clin. Nutr., 2001, 73, 41, 5S–20S.
• 4. Fiordaliso M., Kok N., Desager J.P., Goethals F., Deboyser D., Roberfroid M,. Dietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rats. Lipids, 1995, 30, 163–167.
• 5. Giacco R., Clemente G., Luongo D., Lasorella G., Fiume I., Brouns F., Patti L., Bornet F., Cipriano P., Rivellese A., Riccardi G., Effects of short-chain fructo-oligosaccharides on glucose and lipid metabolism in mild hypercholesterolaemic individuals. Clin. Nutr., 2004, 23, 331–340.
• 6. Grasten S.M., Pajari A.M., Liukkonen K.H., Karppinen S., Mykkanen H.M., Fibers with different solubility characteristics alter similarly the metabolic activity of intestinal microbiota in rats fed cereal brans and inulin. Nutr. Res., 2002, 22, 1435–1444.
• 7. Hollman P.C.H., Evidence for health benefits of plant phenols: local or systemic effects? J. Sci. Food Agric., 2001, 81, 842–852.
• 8. Juśkiewicz J., Zduńczyk Z., Wróblewska M., Oszmiański J., Hernandez T., The response of rats feeding with diets containing grapefruit flavonoid extract. Food Res. Int., 2002, 35, 201–205.
• 9. Juśkiewicz J., Zduńczyk Z., Wróblewska M., The effect of the administration of cellulose and fructans with different degree of polymerization to rats on caecal fermentation and biochemical indicators in the serum. Czech J. Anim. Sci., 2005, 50, 273–280.
• 10. Kok N., Roberfroid M., Delzenne N., Dietary oligofructose modifies the impact of fructose on hepatic triacylglycerol metabolism. Metabolism, 1996, 45, 1547–1550.
• 11. Larrauri J.A., Goni I., Martin-Carron N., Ruperez P., Saura-Calixto F., Measurement of health-promoting properties in fruit dietary fibres: Antioxidant capacity, fermentability and glucose retardation index. J. Sci. Food Agric., 1996, 71, 515–519.
• 12. Lopez-Molina D., Nawarro-Martinez M.D., Melgarejo F.R., Hiner A., Chazarra S., Rodriguez-Lopez J.N., Molecular properties and prebiotic effect of inulin obtained from artichoke (Cynara scolymus L). Phytochemistry, 2005, 66, 1476–1484
• 13. Nicolle C., Cardinault N., Gueux E., Jaffrelo L., Rock E., Mazur A., Amouroux P., Remesy Ch., Health effect of vegetable-based diet: lettuce consumption improves cholesterol metabolism and antioxidant status in the rat. Clin. Nutr., 2004, 23, 605–614.
• 14. Ohkawa H., Ohishi N., Yagi K., Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem., 1979, 95, 351–358.
• 15. Pool-Zobel B., van Loo J., Rowland I., Roberfroid M.B., Experimental evidences on the potential of prebiotic fructans to reduce the risk of colon cancer. Br. J. Nutr., 2002, 87, 273– 281.
• 16. Reeves P.G., Components of the AIN-93 diets as improvements in the AIN-76A diet. J. Nutr., 1997, 127, 838– 841.
• 17. Rowland I.R., Rumney C.J., Coutts J.T., Lievense L.C., Effect of Bifidobacterium longum, and inulin on gut bacterial metabolism, and carcinogen-induced aberrant crypt foci in rats. Carcinogenesis, 1998, 19, 281–285.
• 18. Sangeetha P.T., Ramesh M.N., Prapulla S.G., Recent trends in the microbial production, analysis and application of fructooligosaccharides. Trends Food Sci. Technol., 2005, 16, 442–457.
• 19. Twomey L.N., Pluske J.R, Rowe J.B., Chocz M., Brown W., Pethick D.W., The effects of added fructooligosaccharide (Raftilose®P95) and inulinase on faecal quality and digestibility in dogs. Anim. Feed Sci. Tech., 2003, 108, 83–93.
• 20. Williams C. M., Jackson K. G., Inulin and oligofructose: effects on lipid metabolism from human studies. Br. J. Nutr., 2002, 87, 261–264.
• 21. Willard M.D., Simpson R.P., Delles E.K., Coben N.D., Fossum T.W., Kolp D., Reinhart G., Effects of dietary supplementation of fructo-oligosaccharides on small intestinal bacteria overgrowth in dogs. Am. J. Vet. Res., 1994, 55, 654–659.