Establishment of Rhodiola kirilowii hairy roots using Agrobacterium rhizogenes LBA 9402

• Autorzy: Zych M , Pietrosiuk A. , Karasiewicz M. , Bogacz A. , Kujawski R. , Mrozikiewicz P.M. , Krajewska-Patan A. , Furmanowa M.

Warianty tytułu

PL

Otrzymywanie korzeni wlosnikowatych Rhodiola kirilowii z uzyciem Agrobacterium rhizogenes LBA 9402

• Języki publikacji: EN

Abstrakty

EN

For the first time hairy roots of the Rhodiola genus were obtained by infection of the of Rhodiola kirilowii plantlets with Agrobacterium rhizogenes LBA 9402. First transgenic roots appeared 11 days after infection. The transformation process was confirmed by the polymerase chain reaction (PCR ). DNA analysis showed that rol B gene and aux 1 gene from Ri plasmid of Agrobacterium rhizogenes were incorporated into the plant genome of Rhodiola kirilowii.

PL

Po raz pierwszy korzenie włośnikowate w rodzaju Rhodiola otrzymano na drodze infekcji siewek Rhodiola kirilowii przy użyciu Agrobacterium rhizogenes LBA 9402. Pierwsze korzenie transgeniczne pojawiły się na eksplantatach roślinnych 11 dni po infekcji. Proces transformacji potwierdzono przy użyciu techniki PCR z zastosowaniem specyficznych starterów dla amplifikacji genów rol B, aux 1 i vir D. Analiza DNA wykazała włączenie do genomu rośliny genów rol B i aux 1 z plazmidu Ri Agrobacterium rhizogenes.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2008
• Tom: 54
• Numer: 4
• Opis fizyczny: p.7-16,fig.,ref.

Twórcy

autor

Zych M

• Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Pietrosiuk A.

autor

Karasiewicz M.

autor

Bogacz A.

autor

Kujawski R.

autor

Mrozikiewicz P.M.

autor

Krajewska-Patan A.

autor

Furmanowa M.

Bibliografia

• 1. Ohba H. Rhodiola. In: Eggli U. [ed.]. Crassulaceae. Illustrated handbook of succulent plants. Springer 2003: 210-26.
• 2. Peng JN, Ma CY and Ge YC . Chemical constituents of Rhodiola kirilowii (Regel) Regel. Zhongguo Zhong Yao Za Zhi 1994; 19:676-7, 702.
• 3. K ang S, Zhang J, Lu Y, Lu D. Chemical constituents of Rhodiola kirilowii (Reg.) Reg. Zhongguo Zhong Yao Za Zhi. 1992; 17:100-1, 127.
• 4. Wiedenfeld H, Zych M, Buchwald W, Furmanowa M. New compounds from Rhodiola kirilowii. Sci Pharm 2007; 75:29-34.
• 5. Zhang ZH, Feng SH , Hu GD , Cao Z, Wang LY. Effect of Rhodiola kirilowii (Regel) Maxim on preventing high altitude reactions. A comparison of cardiopulmonary function in villagers at various altitudes. Zhongguo Zhong Yao Za Zhi 1989; 14:687-90, 704.
• 6. Zhang Z, Wang L, Chen Q, Feng S, Cao Z, Zhou X, Hu G, Jiang P, Cao Y. Electron microscopic observation of the effects of Rhodiola kirilowii (Regel) Maxim. in preventing of the rat viscera by hypoxic high altitude environment. Zhongguo Zhong Yao Za Zhi 1990; 15:177 -81, 192.
• 7. Zych M, Furmanowa M, Krajewska-Patan A, Łowicka A, Dreger M, Mendlewska S. Micropropagation of Rhodiola kirilowii plants using encapsulated axillary buds and callus. Acta Biol Cracov Series Bot 2005; 47:83-7.
• 8. T hiem B. In vitro culture techniques in concervation of Rubus chamaemorus L. Acta Biol Cracov Series Bot 2002; 44:181-8.
• 9. U dintsev SN, Krylova SG , Fomina TI . The enhancement of the efficacy of adriamycin by using hepatoprotectors of plant origin in metastases of Ehrlich’s adenocarcinoma to the liver in mice (Russ.). Voprosy Onkologii 1992; 38:1217-22.
• 10. Bocharova OA, Matveev BP, Baryshnikov AI, Figurin KM, Serebriakova RV, Bodarova NB. The effect of a Rhodiola rosea extract on the incidence of recurrences of a superficial bladder cancer (experimental clinical research). Urologija i Nefrologija. 1995; 2:46-7.
• 11. S alikhova RA, Aleksandrova IV, Mazurik VK, Mikhailov VF, Ushenkova LN, Poroshenko GG . Effect of Rhodiola rosea on the yield of mutation alterations and DNA repair in bone marrow cells. Patologicheskaia Fiziologia i èksperimentalnaia Terapia 1997; 4:22-4.
• 12. D uhan OM, Baryliak IR , Nester TI , Dvornyk AS, Kunakh VA. The antimutagenic activity of biomass extracts from the cultured cells of medicinal plants in the Ames test. Tsitol Genet 1999; 33:19-25.
• 13. Maimeskulova LA and Maslov LN. The anti-arrhythmia action of an extract of Rhodiola rosea and of n-tyroso in models of experimental arrhythmias. Eksperimentalnaja Klinicheskaja Farmakologija 1998; 61:37-40.
• 14. Maimeskulova LA, Maslov LN. Anti-arrhythmic effect of phytoadaptogens. Eksperimentaja KliniczeskajaFarmakologia 2000; 63:29-31.
• 15. Li J, Fan WH, Ao H. Effect of Rhodiola on expression of Flt-1, KDR and Tie-2 in rats with ischemic myocardium. Zhoggou Zhong Xi Yi Jie He Za Zhi 2005; 25:445-8.
• 16. G iri A, Narasu L. Transgenic hairy roots: recent trends and applications. Biotechnol Adv 2000; 18:1-22. 17. Wysokińska H. Wykorzystanie kultur korzeni transformowanych do produkcji metabolitów wtórnych. In:Wypijewski K [ed.]. Wybrane zagadnienia biotechnologii roślin. Academic Press UAM, Poznań 1996:11-23.
• 18. Pietrosiuk A, Syklowska-Baranek K, Wiedenfeld H, Wolinowska R, Furmanowa M, Jaroszyk E. The shikonin derivatives and pyrolizidine alkaloids in hairy roots cultures of Lithospermum canescens (Michx.) Lohm. Plant Cell Reports 2006; 25:1052-8.
• 19. Furmanowa M, Sykłowska-Baranek K. Hairy root cultures of Taxus x media var. Hicksii Rehd. as a new source of paclitaxel and 10-deacetylobaccatin III . Biotechnol Lett 2000; 22:683-6.
• 20. G rzegorczyk J, Królicka A, Wysokińska H. Establishment of Salvia officinalis L. hairy root cultures for the production of rosmarinic acid. Zeitschrift für Naturforschung 2006; 61c:351-6.
• 21. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 1962; 15:473-97.
• 22. H ooykaas PJ, Klapwjik PM, Nuti MP, Schilperoort RA, Rorsch A. Transfer of the A. tumefaciens Ti plasmid to avirulent Agrobacteria and Rhizobium ex planta. J Gen Microbiol 1977 ; 98:477 -84.
• 23. G amborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Experiment Cell Res 1968; 50:151-8.
• 24. G upta PK, Durzan DJ . Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) andSugar pine (Pinus lambertiana). Plant Cell Reports 1985; 4:177 -9.
• 25. D oohan FM, Parry DW, Jenkinson P, Nicholson P. The use of species-specific PCR -based assays to analyse Fusarium ear blight of wheat. Plant Pathol 1988; 47:197-205.
• 26. Wasilewska A, Królicka A. Formation and characterization of hairy roots. Review. Biotechnologia 2005; 4:173-88.
• 27. D i Cola A, Constantino P, Spano L. Cell commitment and rolB gene expression in the induction of root differentiation. Plant Cell, Tiss Org Cult 1996; 46:203-9.
• 28. D amiano C, Monticelli S. In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection. Electron J Biotechnol 1998; 1:189-95.
• 29. G iri A, Ravindra ST , Dhingra V, Narasu ML. Influence of different strains of Agrobacterium rhizogenes oninduction of hairy roots and artemisinin production in Artemisia annua. Curr Sci 2001; 81:378-82.
• 30. H u ZB, Alfermann AW. Diterpenoid production in hairy root cultures of Salvia miltiorrhiza. Phytochem 1993; 32:699-703.
• 31. Królicka A, Staniszewska I, Bielawski K, Maliński E, Szafranek J, Łojkowska E. Establishment of hairy roots cultures of Ammi majus. Plant Sci 2001; 160:259-64.