Controversial aspect of using GFP as a marker for the production of transgenic cattle

• Autorzy: Duszewska A. M. , Lipinski D. , Piliszek A. , Slomski R. , Plawski A. , Wojdan J. , Gawron W. , Juzwa W. , Zeyland J. , Wenta-Muchalska E. , Reklewski Z.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to examine the feasibility of identification and selection of cattle embryos based on green fluorescence (GFP-positive) in order to obtain calves carrying an integrated transgene. The construct used (pbLGTNF-EGFP) contained the human tumor necrosis factor alpha (hTNFa) gene fused to the bovine betalactoglobulin promoter (bLG) in plasm id vector pCX-EGFP. In four experiments, 76 zygotes were injected; eight of them developed to the morulac/blastocysts stage of which only five were GFP positive (one of them 100%, one-50%, three- 25%). All of the GFP positive embryos were transferred to recipients. Two calves were born: one after transfer of the 100% GFP positive embryo and the other after transfer of one of the 25% GFP positive embryos. Both animals were healthy with normal weight when compared to two control calves. The integration of pbLGTNF-EGFP in the host genome could not be detected in either of the calves, suggesting that GFP is an unreliable marker for preimplantation screening of embryos.

Słowa kluczowe

EN

green fluorescent protein; pronuclear injection; plasmid vector; bovine beta-lactoglobulin promoter; tumour necrosis factor-alpha; cattle; transgenic cattle; transient expression; green fluorescence; embryo

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2004
• Tom: 07
• Numer: 4
• Opis fizyczny: p.241-249,fig.,ref.

Twórcy

autor

Duszewska A. M.

• Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland

autor

Lipinski D.

autor

Piliszek A.

autor

Slomski R.

autor

Plawski A.

autor

Wojdan J.

autor

Gawron W.

autor

Juzwa W.

autor

Zeyland J.

autor

Wenta-Muchalska E.

autor

Reklewski Z.

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