Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes

• Autorzy: Piao X. , Sun L. , Zhang T. , Gan Y. , Guan Y.
• Języki publikacji: EN

Abstrakty

EN

Effective discrimination of non-complementary nucleotides is an important factor to ensure the accuracy of hybridization-based nucleic acid analyses. The current study investigates the effects of the chemical nature, the positions, the numbers, and the cooperative behavior of mismatches as well as insertions on 20-mer and 30-mer duplexes. We observed the hybridization stability trend affected by mismatches: G:T ≈ G:G > G:A > A:A ≈ T:T > A:C ≈ T:C > C:C. The experimental data show that mismatches at the center of the oligonucleotide probes have a more profound destabilizing effect on the hybridization stability than those at either ends. Insertions also demonstrate a similar destabilizing effect as mismatches. These results provide useful information for designing DNA microarray nucleotide probes and for improving the discrimination accuracy of hybridization-based detections.

Słowa kluczowe

PL

nucleic acid probe; discrimination accuracy; insertion; melting temperature; mismatch effect; hybridization stability; oligonucleotide probe

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 4
• Opis fizyczny: p.713-720,fig.,ref.

Twórcy

autor

Piao X.

• China Medical University, Shenyang 110001, China

autor

Sun L.

autor

Zhang T.

autor

Gan Y.

autor

Guan Y.

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