Charakterystyka toksyny wymiotnej Bacillus cereus

• Autorzy: Berthold A , Doroszkiewicz B.

Warianty tytułu

EN

Characteristics of Bacillus cereus emetic toxin

• Języki publikacji: PL

Abstrakty

EN

Cereulide, an emetic toxin produced by specific Bacillus cereus strains, is neither able to degrade starch, nor can it ferment salicin, and shows weak haemolysis. It is a cyclic low-molecular (1.2 kDa) ring-shaped peptide. Cereulide is resistant to heat (121°C/90 minutes), digestive enzymes, trypsin and pepsin. Pathological symptoms evoked by cereulide are nausea and vomiting, which may occur 1 to 5 hours after the ingestion of a contaminated food product and continue for about 24 hours. Cereulide causes changes in hepatic cell mitochondria (vacuolation) that consequently lead to liver damage. The toxic dose for an adult is 400-500 µg of cereulide. Since it is a very widespread species in the environment, there is a high risk of Bacillus cereus-mediated food poisoning. The main cause of this type of food poisoning are products containing boiled or fried rice and foods rich in starch, such as pasta. Since the condition has a short duration, cases of emetic type food poisoning provoked by cereulide are frequently not even reported to the health service authorities and are therefore highly underestimated in official statistics. The presence of L-leucine, or L-valine aminoacids in a product markedly intensifies the process of the emetic toxin production by Bacillus cereus. Cereulide synthesis is also positively affected by the presence of oxygen. The optimal temperature for cereulide formation ranges from 15 to 20°C, whereas its production at 8-10°C or at a temperature exceeding 35°C is minimal.

Słowa kluczowe

PL

produkty spozywcze; zanieczyszczenia bakteryjne; Bacillus cereus; toksyny bakteryjne; enterotoksyny; toksyna wymiotna; budowa; wytwarzanie; wykrywanie; wystepowanie; zatrucia pokarmowe

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2009
• Tom: 65
• Numer: 01
• Opis fizyczny: s.15-19,bibliogr.

Twórcy

autor

Berthold A

• Szkola Glowna Gospodarstwa Wiejskiego, ul.Nowoursynowska 159 C, 02-787 Warszawa

autor

Doroszkiewicz B.

Bibliografia

• 1.Agata N., Mori M., Ohta M., Suwan S., Ohtani I., Isobe M.: A novel dodecadepsipeptide, cereulide, isolated from Bacillus cereus causes vacule formation in Hep-2 cells. FEMS Microbiol. Lett. 1994, 121, 31-35.
• 2.Agata N,. Ohta M., Mori M., Isobe M.: Production of an emetic toxin, cereulide, is associated with a specific class of Bacillus cereus. Curr. Microbiol. 1996, 33, 67-69.
• 3.Agata N., Ohta M., Yokoyama K.: Production of Bacillus cereus emetic toxin (cereulide) in various foods. J. Clin. Microbiol. 2002, 73, 23-27.
• 4.Andersson M., Jääskeläinen E., Shaheen R., Pirhonen T., Wijnands L., Salkinoja-Salonen M.: Sperm bioassay for rapid detection of cereulide-producing Bacillus cereus in food and related environments. Int. J. Food Microbiol. 2004, 94, 175-183.
• 5.Andersson M., Mikkola R., Helin J., Andersson M., Salkinoja-Salonen M.: A novel sensitive bioassay for detection of Bacillus cereus emetic toxin and related depsipeptide ionophores. Appl. Environm. Microbiol. 1998, 64, 1338-1343.
• 6.Apetroaie C., Andersson M., Spröer C., Tsitko I., Shaheen R., Jääskeläinen E., Wijnands L., Heikkilä R., Salkinoja-Salonen M.: Cereulide-producing strains of Bacillus cereus show diversity. Arch. Microbiol. 2005, 184, 141-151.
• 7.Becker H., Schaller G., Wiese W., Terplan G.: Bacillus cereus infant foods and dried milk products. Int. J. Food Microbiol. 1994, 23, 1-5.
• 8.Berthold A., Molska I.: Występowanie Bacillus cereus w mleku surowym. Żywność. Nauka. Technologia. Jakość. 2002, Supl. (32) 3, 8-12.
• 9.Berthold A., Molska I.: Występowanie Bacillus cereus w środowisku pozyskiwania mleka. Medycyna Wet. 2004, 60, 42-44.
• 10.Berthold A., Gibowicz H.: Występowanie Bacillus cereus w rynkowych produktach mleczarskich. Przegl. Mlecz. 2005, 12, 4-7.
• 11.Dierick K., Van Collie E., Swiecicka I., Meyfroidt G., Devlieger H., Meulemans A., Hoedemaekers G., Fourie L., Heyndrickx M.: Fatal family outbreak of Bacillus cereus - associated food poisoning. J. Clin. Microbiol. 2005, 43, 8, 4277-4279.
• 12.Ehling-Schulz M., Fricker M., Scherer S.: Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay. FEMS Microbiol. Lett. 2004, 232, 189-195.
• 13.Ehling-Schulz M., Vukov N., Schulz A., Shaheen R., Andersson M., Märtlbauer E.: Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus. Appl. Environm. Microbiol. 2005, 71, 1, 105-113.
• 14.Finlay W., Logan N., Sutherland A.: Bacillus cereus produces most emetic toxin at lower temperatures. Lett. Appl. Microbiol. 2000, 3, 385-389.
• 15.Finlay W., Logan N., Sutherland A.: Semiautomated metabolic staining assay for Bacillus cereus emetic toxin. Appl. Environm. Microbiol. 1999, 65, 4, 1811-1812.
• 16.Granum P.: Bacillus cereus, [w:] Fratamico P., Bhunie A., Smith J. (red.): Foodborne Pathogens. Microbiology and Molecular Biology. Caister Academic Press Norfolk 2005, 409-419.
• 17.Häggblom M., Apetroaie C., Andersson M., Salkinoja-Salonen M.: Quantitative analysis of cereulide, the emetic toxin of Bacillus cereus, produced under various conditions. Appl. Environm. Microbiol. 2002, 68, 2479-2483.
• 18.Hoornstra D., Andersson M., Mikkola R., Salkinoja-Salonen M.: A new method for in vitro detection of microbially produced mitochondrial toxins. Toxicology in Vitro 2003, 17, 745-751.
• 19.Hughes S., Bartholomew B., Hardy J., Kramer J.: Potential application of a Hep-2 cell assay in the investigation of Bacillus cereus emetic-syndrome food poisoning. FEMS Microbiol. Lett. 1989, 52, 7-12.
• 20.Jääskeläinen E., Häggblom M., Andersson M., Salkinoja-Salonen M.: Atmospheric oxygen and other conditions affecting the production of cereulide by Bacillus cereus in food. Int. J. Food Microbiol. 2004, 96, 75-83.
• 21.Jääskeläinen E., Häggblom M., Andersson M., Vanne L., Salkinoja-Salonen M.: Potential of bakery products for producing cereulide, the Bacillus cereus emetic toxin: quantitative analysis by chemical and biological methods. J. Food Prot. 2003, 66, 6, 1047-1054.
• 22.Jääskeläinen E., Teplova V., Andersson M., Andersson L., Tammela P., Andersson M., Pirhonen T., Saris N., Vuorela P., Salkinoja-Salonen M.: In vitro assay for human toxicity of cereulide, the emetic toxin produced by food poisoning Bacillus cereus. Toxicology In Vitro 2003, 17, 737-744.
• 23.Kleer J., Bartholomä A., Levetzow R., Reiche T., Sinell H., Teufel P.: Bakterielle Lebensmittel-Infektionen und Intoxikationen in Einrichtungen zur Gemeinschaftsverpflegung 1985 bis 2000. Arch. Lebensm. Hyg. 2001, 73, 112-116.
• 24.Kawamura-Sato K., Hirama Y., Agata N., Ito H., Torii K., Takeno A., Hasegawa T., Shimomura Y.: Quantitative analysis of cereulide, an emetic toxin of Bacillus cereus, by using rat liver mitochondria. Microbiol. Immunol. 2005, 49, 1, 25-30.
• 25.Kuse M., Franz T., Koga K., Suwan S., Isobe M., Agata N., Ohta M.: High incorporation of L-aminoacids to cereulide, an emetic toxin from Bacillus cereus. Bioorganic and Medicinal Chem. Lett. 2000, 10, 735-739.
• 26.Lund T., De Buyser M., Granum P.: A new cytotoxin from Bacillus cereus that may cause necrotic enteritis. Molecular Microbiol. 2000, 38, 254-261.
• 27.Mahler H., Pasi A., Kramer J., Schulte P., Scoging A., Bar W., Krahenbuhl S.: Fulminant liver failure in association with the emetic toxin of Bacillus cereus. New England J. Med. 1997, 336, 1142-1148.
• 28.Pirttijärvi T., Andersson M., Scoging A., Salkinoja-Salonen M.: Evaluation of methods for recognizing strains of the Bacillus cereus group with food poisoning potential among industrial and environmental contaminants. Syst. Appl. Microbiol. 1999, 22, 133-144.
• 29.Shaheen R., Andersson M., Apetroaie C., Schulz A., Ehling-Schulz M., Ollilainen V., Salkinoja-Salonen M.: Potential of selected infant food formulas for production of Bacillus cereus emetic toxin, cereulide. Int. J. Food Microbiol. 2006, 107, 287-294.
• 30.Svensson B., Monthán A., Shaheen R., Andersson M., Salkinoja-Salonen M., Christiansson A.: Occurrence of emetic toxin producing Bacillus cereus in the dairy production chain. Int. Dairy J. 2006, 16, 740-749.
• 31.Toh M., Moffitt M., Henrichsen L., Raftery M., Barrow M., Cox J., Marquis C., Neilan B.: Cereulide, the emetic toxin of Bacillus cereus, is putatively a product of nonribosomal peptide synthesis. J. Appl. Microbiol. 2004, 97, 992-1000.