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1999 | 46 | 3 |

Tytuł artykułu

Factors improving the accuracy of determination of 15N relaxation parameters in proteins

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A number of factors at all stages of data processing which affect the accuracy of determination of 15N relaxation parameters in 15N-labeled proteins is discussed. Methods which allow to improve accuracy of the determined parameters are presented using data obtained for Cucurbita maxima trypsin inhibitor.

Wydawca

-

Rocznik

Tom

46

Numer

3

Opis fizyczny

p.665-671,fig.

Twórcy

autor
  • Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland
autor

Bibliografia

  • 1. Williams, RJ.P. (1989) NMR studies of mobil­ity within protein structure. Eur. J. Biochem. 183, 479-497.
  • 2. Pabo, C.O. & Sauer, R.T. (1992) Transcription factors: Structural families and principles of DNA recognition. Annu. Rev. Biochem. 61, 1053-1095.
  • 3. Barbato, G., Ikura, M., Kay, L.E., Pastor, R.W. & Bax, A. (1992) Backbone dynamics of cal­modulin studied by 15N relaxation using in­verse detected two-dimensional NMR spec­troscopy: The central helix is flexible. Biochem­istry 31, 5269-5278.
  • 4. Peng, J.W. & Wagner, G. (1992) Mapping of spectral density functions using heteronuclear NMR relaxation measurements. J. Magn. Reson. 98, 308-332.
  • 5. Kay, L.E., Torchia, D.A., & Bax, A. (1989) Backbone dynamics of proteins as studied by loN inverse detected heteronuclear NMR spec­troscopy: Application to staphylococcal nucle­ase. Biochemistry 28, 8972-8979.
  • 6. Clore, G.M., Driscoll, P.C., Wingfield, P.T. & Gronenborn, A.M. (1990) Analysis of the back­bone dynamics of interleukin-1/? using two-di­mensional inverse detected heteronuclear H NMR spectroscopy. Biochemistry 29, 7387-7401.
  • 7. Weiss, G.H. & Ferretti, J.A. (1983) Accuracy and precision in the estimation of peak areas and NOE factors. J. Magn. Reson. 55, 397- 407.
  • 8. Weiss, G.H., Ferretti, J.A. & Byrd, R.A. (1987) Accuracy and precision in the estimation of peak areas and NOE factors. II. The effects of apodization. J. Magn. Reson. 71, 97-105.
  • 9. Weiss, G.H. & Ferretti, J.A. (1988) Optimal design of relaxation time experiments. Prog. NMR Spectrosc. 20, 317-335.
  • 10. Gryff-Keller, A., Ejchart, A. & Jackowski, K. (1994) Shielding anisotropics of acetic car­bons and solution molecular dynamics ofethynyltrimethylsilane as studied by C nu­clear spin relaxation and theoretical ab initio calculation. J. Magn. Reson. A 111, 186-190.
  • 11. Kowalewski, J., Ericsson, A. & Vestin, R. (1978) Determinatipn of NOE factors using the dynamic Overhauser enhancement tech­nique combined with a nonlinear least-squa­res-fitting procedure. J. Magn. Reson. 31, 165-169.
  • 12. Bolewska, K., Krowarsch, D., Otlewski. J., Jaroszewski, L. & Bierzyriski, A. (1995) Syn­thesis, cloning and expression in Escherichia coll of a gene coding for the Met8-Leu CMTI-I — a representative of the squash inhibitors of serine proteinases FEBSLett. 377,172-174.
  • 13. Kay, L.E., Keifer, P. & Saarinen, T. (1992) Pure absorption gradient enhanced hetero­nuclear single quantum correlation spectros­copy with improved sensitivity. J. Am, Chem. Soc. 114, 10663-10665.
  • 14. Farrow, N.A., Muhandiram, R., Singer, A.U., Pascal, S.M., Kay, C.M., Gish, G., Shoelton, S.E., Pawson, T., Forman-Kay, J.D. & Kay, L.E. (1994) Backbone dynamics of a free and a phosphopeptide-complexed Src homology 2 domain studied by NMR relaxation. Bio­chemistry 33, 5y»4-bOU3.
  • 15. Kay, L.E., Nicholson, L.K., Delaglio, F., Bax, A. & Torchia, A. (1992) Pulse schemes for re­moval of the effects of cross correlation be­tween dipolar and chemical-shift anisotropy relaxation mechanisms on the measurement of heteronuclear Tj and T2 values in proteins. J. Magn. Reson. 97, 359-375.
  • 16. States, D.J., Habercorn, H.A. & Ruben, D.J. (1982) A two-dimensional nuclear Overhauser experiment with pure absorptive phase in four quadrants. J. Magn. Reson. 48, 286-292.
  • 17. Shaka, A.J., Baker, P.B. & Freeman. R. (1985) Computer-optimized decoupling scheme for wideband applications and low-level opera­tion. J. Magn. Reson. 64, 547-552.
  • 18. Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J. & Bax. A. (1995) NMRPipe: A multidimensional spectral processing system based on UNIX pipes. J. BiomoL NMR 6, 277-293.
  • 19. Bartels, C., Xia, T., Billeter, M., Güntert, P. & Wüthrich, K. (1995) The program XEASY for computer-supported NMR spectral analysis of biological macromolecules J. Biomol. NMR 6, 1-10.
  • 20. Cavanagh, J., Fairbrother, WJ., Palmer III, A.G. & Skelton, N J. (1996) Protein NMR Spec­troscopy. Principles and Practice. Academic Press, San Diego.
  • 21. Bremi, T., Ernst, M. & Ernst, R.R. (1994) Side-chain motion with two degrees of free­dom in peptides. An NMR study of phenyl­alanine side chains in antamanide. J. Phys. Chem. 98, 9322-9334.
  • 22. Rischel, C., Madsen, J.Chr., Andersen, K.V. & Poulsen, F.M. (1994) Comparison of backbone dynamics of apo- and holo-acyl-coenzyme A binding protein using 15n relaxation measure­ments. Biochemistry 33, 13997-14002.
  • 23. Remerowski, M.L., Pepermans, H.A.M., Hil- bers, C.W. & van de Ven, F.J.M. (1996) Back­bone dynamics of the 269-residue protease Savinase determined from 15N NMR relax­ation measurements. Eur. J. Biochem. 235, 629-640.
  • 24. Canet, D. (1976) Systematic errors due to im­proper waiting times in heteronuclear Over­hauser effect measurements by the gated de­coupling technique. J. Magn. Reson. 23, 361- 364.

Typ dokumentu

Bibliografia

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