Analysis of individual azurocidin N-glycosylation sites in regard to its secretion by insect cells, susceptibility to proteolysis and antibacterial activity

• Autorzy: Indyk K. , Olczak T. , Ciuraszkiewicz J. , Watorek W. , Olczak M.
• Języki publikacji: EN

Abstrakty

EN

Azurocidin is an inactive serine protease homolog with primary sequence similarity to neutrophil elastase, cathepsin G, and proteinase 3. The aim of this study was to investigate possible consequences of differential glycosylation of azurocidin in regard to its secretion, protein stability as measured by susceptibility to proteolysis, and antibacterial activity. Site-directed mutagenesis was employed to generate mutant azurocidin variants lacking individual N-glycosylation sites. Our results show that N-linked glycans may play a role in proper azurocidin folding and subsequent secretion by insect cells. We also demonstrate that N-linked glycosylation contributes to azurocidin stability by protecting it from proteolysis. The lack of N-glycosylation at individual sites does not significantly influence the azurocidin antibacterial activity.

Słowa kluczowe

EN

protein secretion; proteolysis; susceptibility; antibacterial activity; glycosylation; azurocidin; antimicrobial activity; insect cell

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 3
• Opis fizyczny: p.567-573,fig.,ref.

Twórcy

autor

Indyk K.

• University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland

autor

Olczak T.

autor

Ciuraszkiewicz J.

autor

Watorek W.

autor

Olczak M.

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