Specific identification of Escherichia coli O157 using multiplex PCR

• Autorzy: Osek J
• Języki publikacji: EN

Abstrakty

EN

A multiplex PCR was developed to identify E.coli O157 serogroup among other pathogenic and non-pathogenic E.coli serotypes as well as to differentiate them from other Gram-negative bacteria. This multiplex PCR, amplifying genes encoding the universal stress protein (uspA) and the O157 antigen-encoding operon (rfb) of E.coli, allowed a simultaneous identification of the 884 bp and 420 bp amplicons, respectively, in all O157-positive E.coli whereas no amplification products were detected in non-E.coli bacteria. The test will be useful for the rapid and specific identification of E.coli O157 bacteria responsible for human and animal diarrheal and systemic disorders.

Słowa kluczowe

EN

bacteria; Escherichia coli; polymerase chain reaction; hemorrhagic colitis; identification

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2000
• Tom: 44
• Numer: 1
• Opis fizyczny: p.39-44,fig.

Twórcy

autor

Osek J

• National Veterinary Research Institute, 24-100 Pulawy, Poland

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