Occurrence of Lawsonia intracellularis and Brachyspira spp. infection in swine suffering from diarrhoea

• Autorzy: Plawinska J. , Jakubowski T. , Rzewuska M. , Binek M.
• Języki publikacji: EN

Abstrakty

EN

Principal aim of this study was to examine fecal samples from pigs suffering from diarrhea for the presence of Lawsonia intracellularis, Brachyspira hyodysenteriae and Brachyspira pilosicoli. The molecular techniques such as PCR and nested PCR were employed to detect the presence of p78 fragment of genomic DNA specific for Lawsonia intracellularis as well as fragment of tlyA gene specific for Brachyspira hyodysenteriae and 16S rDNA gene of Brachyspira pilosicoli. We assumed that about 25% of pigs were infected with Lawsonia intracellularis, about 10% with Brachyspira hyodysenteriae and only 0,8% with Brachyspira pilosicoli. In about 3% mixed infection with L. intracellularis and B. hyodysenteriae was observed. Results were comparable in herds that differed in quantity, breeding technology, hygienic standards and preventive treatment with different chemotherapeutics.

Słowa kluczowe

EN

pig; infection; Brachyspira pilosicoli; Lawsonia intracellularis; occurrence; Brachyspira hyodysenteriae; diarrhea; porcine proliferative enteropathy

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2004
• Tom: 07
• Numer: 3
• Opis fizyczny: p.203-206,fig.,ref.

Twórcy

autor

Plawinska J.

• Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Jakubowski T.

autor

Rzewuska M.

autor

Binek M.

Bibliografia

• Hampson DJ, Stanton TB (1997) Characteristics of intes­tinal spirochaetes. In: Hampson DJ , Stanton TB (eds) Intestinal spirochaetes in domestic animals and hu­mans. Cab International, pp 7-47.
• Hampson DJ, Trott DJ (1999) Spirochetal diarrhea/por- cine intestinal spirochetosis. In: Straw BE, D’Allaire S, Mengeling WL, Taylor DJ (eds) Diseases of Swine. 8th ed., Iowa State University Press, Ames, IA, pp 553-564.
• McOrist S, Roberts L, Jasni S, Rowland CA, Lawson GHK, Gebhard CJ, Bosworth B (1996) Developed and resolving lesions in porcine proliferative enteropathy: possible pathogenic mechanisms. J Comp Path, 115: 35-45.
• Joens LA, Nibbelink S, Glock RD (1997) Induction of gross and microscopic lesion of porcine proliferative enteritis by Lawsonia intracellularis. Am J Vet Res, 58: 1125-1131.
• Jones GF, Davies PR, Rose R, Ward GE, Murtaugh MP (1993) Comparison of techniques for diagnosis of pro­liferative enteritis of swine. Am J Vet Res 54: 1980-1985.
• Pejsak Z, Zmudzki J, Stankevicius A (2001) Detection of Lawsonia intracellularis by one tube nested - PCR. Medycyna Wet 57: 723-726.
• Rzewuska M, Jakubowski T, Kizerwetter M, Binek M (2001) Detection of Brachyspira sp. in samples of swine faeces based on culture method and on PCR technique. Cell Mol Biol Lett 6: 813-814.