The activity of alpha 1,6-fucosyltransferase during human megakaryocytic differentiation

• Autorzy: Bany-Laszewicz U , Kaminska J. , Klimczak-Jajor E. , Koscielak J.
• Języki publikacji: EN

Abstrakty

EN

α1,6-Fucosyltransferase (6FucT, E.C. 2.4.1.68) is one of the enzymes involved in the synthesis of N-linked glycans of the GpIIb/IIIa complex (CD41a) which is present on megakaryocytes (MKs) and platelets. In this study, we examined 6FucT activity in ex vivo cultures of immunoselected cord blood CD34+ cells grown in a medium promoting megakaryocytopoiesis. Our results show that the activity of 6FucT increased ahead of, and thereafter concomitantly with, cells expressing the CD41a antigen. When the CD41a+ subpopulation of cells was immunoselected (using anti-CD61 i.e. anti-GpIIIa antibodies), its 6FucT activity increased proportionally to the yield of CD61+ cells. Taking into account the heavy load of 6FucT in platelets and megakaryocytes, we regard this enzyme as a candidate for the earliest marker of MK-commitment in cultured hematopoietic stem cells. Such a marker should allow an earlier detection and earlier transplantation of patients’ own, ex vivo expanded, Mk progenitors.

Słowa kluczowe

EN

glycoprotein; thrombocytopenia; N-linked glycan; megakaryocyte; enzyme; synthesis; cancer; platelet; alpha 1,6-fucosylotransferase

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 1
• Opis fizyczny: p.145-152,fig.,ref.

Twórcy

autor

Bany-Laszewicz U

• Institute of Hematology and Blood Transfusion, Chocimska 5, 00-957 Warsaw, Poland

autor

Kaminska J.

autor

Klimczak-Jajor E.

autor

Koscielak J.

Bibliografia

• 1. Hassan, H.T. and Zander, A. Thrombocytopenia after high-dose chemotherapy and autologous stem cell transplantation: An unresolved problem and possible approaches to resolve it. J. Hematother. 5 (1996) 407-414.
• 2. Ozer, H. Clinical Issues and Treatment Advances in Thrombocytopenia: Introduction. Semin. Hematol. 37 (2000) (suppl 4)1-2.
• 3. Scheding, S., Kratz-Albers, K., Meister, B., Brugger, W. and Kanz, L. Ex vivo expansion of hematopoietic progenitor cells for clinical use. Semin. Hematol. 35 (1998) 232-240.
• 4. Long, M. Megakaryocyte differentiation events. Semin. Hematol. 35 (1998) 192-199.
• 5. Vainchenker, W. and Kieffer, N. Human megakaryocytopoiesis: In vitro regulation and characterization of megakaryocytic precursor cells by differentiation markers. Blood Rev. 2 (1988) 102-107.
• 6. Kościelak, J., Antoniewicz-Papis, J., Zdebska, E., Maj, S. and Leszko, B. Activity of platelet alpha-6-fucosyltransferase is inversely related to blood platelet concentration. Acta Biochim. Pol. 42 (1995) 35-39.
• 7. Kościelak, J., Pacuszka, T., Kubin, J. and Zdziechowska, H. Serum α-6- fucozyltransferase is released from platelets during clotting of blood. Glycoconjugate J. 4 (1987) 43-44.
• 8. Antoniewicz, J., Bykowska, K., Zdebska, E. and Kościelak, J. Human platelets release α-6-fucosyltransferase upon activation. FEBS Lett. 244 (1989) 388-390.
• 9. Kamińska, J., Musielak, M., Nowicka, A., Woźniewicz, B. and Kościelak, J. Neutrophils promote the release of α-6-fucosyltransferase from blood platelets through the action of cathepsin G and elastase. Biochimie 83 (2001) 739-742.
• 10. Ratajczak, M.Z., Ratajczak, J., Machaliński, B., Mick, R.M. and Gewirtz, A.M. In vitro and in vivo evidence that ex vivo cytokine priming of donor marrow cells may ameliorate posttransplant thrombocytopenia. Blood 91 (1998) 353-359.
• 11. Debili, N., Hegyi, E., Navarro, S., Katz, A., Mouthon, M.A. and Breton-Gorius, J. and Vainchenker, W. In vitro effects of hematopoietic growth factors on the proliferation, endoreplication, and maturation of human megakaryocytes. Blood 77 (1991) 2326-2338.
• 12. Kamińska, J., Glick, M.C. and Kościelak, J. Purification and characterisation of GDP-L-Fuc: N-acetyl β-D-glucosaminide α1→6fucosyltransferase from human blood platelets. Glycoconjugate J. 15 (1998) 783-788.
• 13. Zieleński, J. and Kościelak, J. Enzymatic synthesis of neolactotetra osylceramide by N-acetyllactosamine synthase of human serum. Eur. J. Biochem. 125 (1982) 323-329.
• 14. Kuter, D.J. Megakaryopoiesis and thrombopoiesis. in: Williams Hematology, (Beutler, E., Lichtman, M.A., Coller, B.S., Kipps, T.J. and Seligsohn, U., Eds), 6th edition, McGraw Hill Companies, 2001, 1339-1355.