Cytology and fertility of viable hybrids of Nicotiana tabacum L. cv.TB-566 with N. alata Link et Otto

• Autorzy: Laskowska D , Berbec A
• Języki publikacji: EN

Abstrakty

EN

A vigorous hybrid (N. tabacum cv. TB-566 tetra x N. alata) x N. alata was obtained by backcrossing a partly viable sesquidiploid hybrid N. tabacum cv. TB-566 tetra x N. alata to N. alata. The hybrid was a 35-chromosome near-amphihaploid with a pair of N. alata chromosomes in disomic condition. It was completely self- and cross-sterile and formed from 7 to 8 bivalents in pollen mother cells. By using stem pith culture, polyploidized regenerants were obtained from the 35-chromosome hybrid with somatic chromosome numbers from 65 to 70. These regenerants showed fairly regular meiosis with the number of bivalents in pollen mother cells ranging from 27.3 to 30.4. Irregularities in meiosis included a high number of univalents, aberrant tetrads, and a high frequency of micronuclei. The percentage of acetocarmine-stainable pollen ranged from 22.1 to 78.4. A 66-chromosome regenerant showed fairly regular meiosis and was self-fertile but could not be backcrossed to N. tabacum. This barrier seems to be caused by genic imbalance rather than irregularities of meiotic divisions. Hence transfer programs based on the introgression of entire linkage groups (sexual and somatic hybridization) seem to be of little use in the case of that species.

Słowa kluczowe

EN

Nicotiana alata; amphihaploid; mitosis; interspecific hybridization; amphidiploid; breeding technique; plant breeding; TB-566 cultivar; hybrid; genetic engineering; tobacco; chromosome pairing; Nicotiana tabacum

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2005
• Tom: 46
• Numer: 1
• Opis fizyczny: p.11-18,fig.,ref.

Twórcy

autor

Laskowska D

• Institute of Soil Science and Plant Cultivation, Special Crops Department, Czartoryskich 8, 24-100 Pulawy, Poland

autor

Berbec A

• Institute of Soil Science and Plant Cultivation, Special Crops Department, Czartoryskich 8, 24-100 Pulawy, Poland

Bibliografia

• Berbeć A, 1987. Cytogenetical study on Nicotiana tabacum L. cv. Nadwiślański Mały (2x and 4x) x Nicotiana alata Link et Otto hybrids. Genet Pol 28: 251-262.
• Berbeć A, 1988. Morphology, cytogenetics and resistance of amphidiploid Nicotiana raimondii Macbride x N. tabacum L. (F₁ cv. Zamojska 4 cv.LB-838) to Potato Virus Y. Genet Pol 29:41-52.
• Burns JA, 1964. A technique for making preparations of mitotic chromosomes from Nicotiana flowers. Tob Sci 8: 1-2.
• Burns JA, 1982. The chromosomes of Nicotiana africana Merxm.: a recently discovered species. Hered J 73: 115-118.
• Chaleff RS, Ray TB, 1984. Herbicide-resistant mutants from tobacco cell cultures. Science 223: 1148-1151.
• Chaplin JF„ Mann TJ, 1961. Interspecific hybridization, gene transfer and chromosome substitution in Nicotiana. N. Carolina State Coll AgrExp Sta Tech Bul 145: 1—31.
• Clayton EE, 1954. Identifying disease resistance suited to interspecific transfer. Hered J 45: 273-277.
• Dimanov D, Atanassov A, 1989. A study of possibility to apply UV light in experiments of somatic hybridization in protoplast cultures of Nicotiana alata and Nicotiana tabacum (line Virginia 89). Genet Sci 22-1:40-44.
• Doroszewska T, Berbeć A, 1996. Chromosome pairing and microsporogenesis in interspecific F₁ hybrids of N. africana with different cultivars of N. tabacum. J Genet Breed 50: 75-82.
• Doroszewska T, Berbeć A, 2000. Cytogenetical investigations of polyploid interspecific hybrids of Nicotiana africana with different cultivars of N. tabacum. J Genet Breed 50: 75-82.
• Douglas GC, Keller WA, Setterfield G, 1981. Somatic hybridization between Nicotiana rustica and N. tabacum II. Protoplast fusion and selection and regeneration of hybrid plants. Can J Bot 59: 1509-1513.
• Gajos Z, 1981. Przeniesienie odporności na wirus brązowej plamistości pomidora (Tomato Spotted Wilt Vims) z Nicotiana alata Link et Otto do tytoniu szlachetnego poprzez skrzyżowanie obu gatunków. [Transmission of resistance to Tomato Spotted Wilt Virus from Nicotiana alata Link et Otto to N. tabacum by crossing both species.] Biul CLPT 1-2: 3-24.
• Gajos Z, 1988. Polalta - odmiana tytoniu odporna na wirus brązowej plamistości pomidora (TSWV) i czarną zgniliznę korzeni ( Thielaviopsis basicola Ferr.). [Polalta - a tobacco cultivar resistant to Tomato Spotted Wilt Vims and black root rot Thielaviopsis basicola Ferr.]. Biul CLPT 1-4:7-25.
• Gerstel DU, 1960. Segregation in new allopolyploids of Nicotiana. I. Comparison of 6x (N. tabacum x N. tomentosiformis) and 6x (N. tabacum x N. otophora). Genetics 45: 1723-1734.
• Goodspeed TH, Clausen RE, 1928. Interspecific hybridization in Nicotiana. VIII. The sylvestris- tomentosa-tabacum hybrid triangle and its bearing on the origin of N. tabacum. University of California publication, Botany 11: 245-256.
• Goodspeed TH, 1954. The genus Nicotiana. Chronica Botanica. Waltham, Mass., USA.
• Kostoff D, 1943. Cytogenetics of the genus Nicotiana. State Printing House, Sofia.
• Linsmaier EM, Skoog F, 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plantarum 18: 100-127.
• Lloyd R, 1975. Tissue culture as a means of circumventing lethality in an interspecific Nicotiana hybrid. Tob Science 19: 4-6.
• Malepszy S, Niemirowicz-Szczytt K, Przybyłecki Z, 1989. Biotechnologia w genetyce i hodowli roślin. [Biotechnology in genetics and plant breeding.] PWN, Warszawa.
• Patrascu M, Paunescu AD, 1997. Transfer of the resistance to TSWV from Nicotiana alata to other tobacco cultivars using in vitro culture techniques. Coresta Inf Bul: 53.
• Pop IP, 1979. Reaction of some Nicotiana sp. and tobacco varieties to artificial inoculation with Tomato Spotted Wilt Virus. Zesz Probl Post Nauk Rol 226: 17-26.
• Stoyanova MP, 1979. Resultats de L’Hybridation Entre les Especes Nicotiana tabacum L. et Nicotiana alata Link. Comptes rendus de l’Academie bulgare des Sciences 32(5): 683-686.