Comparison of the sensitivity of antibiotic residue screening methods to sulphonamide standards and their presumptive identification by para-aminobenzoic acid

• Autorzy: Janosova J , Kozarova I. , Mate D. , Okerman L.
• Języki publikacji: EN

Abstrakty

EN

The sensitivity of three microbiological antibiotic residue screening methods - the Four Plate Test (FPT), the Screening Test for Antibiotic Residues (STAR) and the Premi®Test - was compared for the detection of 10 different standards from the sulphonamide (SA) group. Phtalylsulphathiazole (PHT), sulphadimidine (SD), sulphaguanidine (SG), sulphachlorpyridazine (SCHP), sulphamerazine (SRZ), sulphamethoxazole (SMX), sulphanilamid (SAM), sulphanilic acid (SAC), sulphaquinoxaline (SQ) and sulphathiazole (STZ) were tested using the concentrations from 0.05 µg.ml⁻¹ to 1 µg.ml⁻¹. The detection sensitivity of the methods represented by minimum inhibiting concentrations (MIC) of SA standards was evaluated. The MIC of SAs represent the detection limits of methods (LOD) for individual substances. The MIC of SA standards detected by the FPT was 0.2 µg.ml⁻¹ for SMX, STZ, SQ and SRZ, 0.3 µg.ml⁻¹ for SCHP, and 0.4 µg.ml⁻¹ for SD. The MIC of SA standards detected by the STAR was 0.05 µg.ml⁻¹ for SMX, STZ, SQ and SCHP, 0.1 µg.ml⁻¹ for SRZ, and 0.3 µg.ml⁻¹ for SD. No detection sensitivity of either method was observed for SAM, SAC, SG and PHT standards. The MIC of SA standards detected by Premi®Test was 0.05 µg.ml⁻¹ for SMX, STZ, SQ, SCHP, SRZ and SD, 0.1 µg.ml⁻¹ for PHT, 0.2 µg.ml⁻¹ for SAM, and 0.3 µg.ml⁻¹ for SAC and SG. The results of the tests showed that Premi®Test is the most sensitive method to detect Sas, followed by the STAR and the FPT methods. The inhibitory effect of para-aminobenzoic acid (PABA) toward the residual concentrations of SA standards was evaluated. The reference PABA concentrations for the confirmation of the presence of all SAs detected by the FPT and the STAR was 1 µg.ml⁻¹ and 10 µg.ml⁻¹ for the Premi®Test. In the authors’ opinion, the presented concentration of PABA can be recommended for a reliable confirmation of the presence of SA residues even at concentrations that present a potential risk to human health.

Słowa kluczowe

EN

para-aminobenzoic acid; veterinary drug; medicine; sulphonamide standard; food; monitoring; sulphonamide residue; identification; residue; veterinary medicine; antibiotic residue; detection; microbiological method

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 05
• Opis fizyczny: p.663-667,fig.,ref.

Twórcy

autor

Janosova J

• University of Veterinary Medicine, Komenskeho 73, 040 01 Kosice, Slovakia

autor

Kozarova I.

autor

Mate D.

autor

Okerman L.

Bibliografia

• 1.An official method for laboratory diagnostics of food and feed CH 12.16.: Detection of the residues of inhibitory substances. The Four Plate Test (Bogaerts and Wolf, 1980) (In Slovak). Bull. of the Ministry of Agriculture of the Slovak Republic 2004, 36, part 1, 277-284.
• 2.An official method for laboratory diagnostics of food and feed CH 12.18.: Determination of residues of inhibitory substances in meat by the Premi®Test (In Slovak). Bull. of the Ministry of Agriculture of the Slovak Republic 2004, 36, part 11, 20-23.
• 3.An official method for laboratory diagnostics of food and feed CH 12.19.: Screening test for determination of antibiotic residues using bacterial strains (STAR) (In Slovak). Bull. of the Ministry of Agriculture of the Slovak Republic 2004, 36, part 11, 24-36.
• 4.Bogaerts R., Wolf F.: A standardised method for the detection of residues of antibacterial substances in fresh meat. Fleischwirtschaft 1980, 60, 672-673.
• 5.Braham R., Black W. D., Claxton J., Yee J.: A rapid assay for detecting sulfonamides in tissues of slaughtered animals. J. Food Prot. 2001, 64, 1565-1573.
• 6.Council Regulation (EEC) No. 2377/90 of 26 June 1990 laying down a Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Official Journal L 224, 18. 8. 1990, 1-8.
• 7.Ferrini A. M., Mannoni V., Aureli P.: The Combinated Plates Microbial Assay (CPMA) technique for the detection and presumptive identification of β-lactam, sulfonamide, streptomycin and tetracycline residues in meat. Arch. Lebensmittelhyg. 1997, 48, 121-144.
• 8.Gaudin V., Maris P., Fuselier R., Ribouchon J. L., Cadieu N., Rault A.: Validation of a microbiological method: the STAR protocol, a five-plate test, for the screening of antibiotic residues in milk. Food Addit. Contam. 2004, 21, 422-433.
• 9.Gudding R.: An improved bacteriological method for the detection of sulfonamide residues in food. Acta Vet. Scand. 1976, 17, 458-464.
• 10.Gudding R.: The suitability of some media and peptones for sulfonamide testing. Acta Vet. Scand. 1974, 15, 366-380.
• 11.Koréneková B., Skalická M., Nad' P.: Zinc in cattle from long-term emission polluted area. Bull. Environ. Contam. Toxicol. 2006, 76, 4, 684-688.
• 12.Kožárová I., Labanská D.: Comparison study of estimation of detection sensitiveness of microbial inhibitory tests used for screening of sulphonamide in practise. Proc. Internat. Conf. Risk factors of food chain V. Nitra, SR, 2005 October 6, pp. 162-166.
• 13.Kožárová I., Máté D., Cabadaj R.: Methods for the determination of sulphonamide residues in milk and other animal products. Proc. Internat. Conf. Hygiena Alimentorum XXII. Štrbské Pleso, High Tatras, SR, 2001 June 5-7, pp. 159-163.
• 14.Kožárová I., Máté D., Cabadaj R., Hussein K.: Methods for determining sulphonamide residues in meat. Proc. Internat. Conf. Hygiena Alimentorum XXIII. Štrbské Pleso, High Tatras, SR, 2002 June 5-7, pp. 65-67.
• 15.Lukáč N., Massányi P.: Effects of trace elements on the immune system. Epidemiologie, Mikrobiologie, Imunologie 2007, 56, 3-9.
• 16.Marcinčák S., Hussein K., Popelka P., Zdolec N.: The evaluation of the Premi®Test used for the detection of sulphadimidine residues in eggs. Folia Vet. 2005, 49, 161-164.
• 17.Prescott J. F., Baggot J. D.: Antimicrobial Therapy in Veterinary Medicine. Ames, Iowa State University Press 1993, 564-565.
• 18.Salem D. A.: Estamination of antibiotics, sulphonamides and nitrofurans residues in chicken meat. Assiut Vet. Med. J. 1998, 39, 192-200.
• 19.Stead S., Sharman J. A., Tarbin J. A., Gibson E., Richmond S., Stark J.: Meeting maximum residue limits: an improved screening technique for rapid detection of antimicrobial residues in animal food products. Food Addit. Contam. 2004, 21, 216-221.