Obtaining carrot [Daucus carota L.] plants in isolated microspore cultures

• Autorzy: Gorecka K , Kowalska U , Krzyzanowska D , Kiszczak W
• Języki publikacji: EN

Abstrakty

EN

Microspores were cultured on the modified B₅ liquid medium containing 2.4D (0.1 mg L⁻¹), NAA (0.1 mg L ⁻¹), L-glutamine (500 mg L⁻¹), L-serine (100 mg L⁻¹), and sucrose (100 g L⁻¹). The developmental stages of microspores and divisions were observed. Initially, the formation of binuclear and multicellular structures was noticed. Plants regenerated in the cultures in which the tetrad stage of microsporogenesis had predominated. Embryoids were still forming 24 weeks after the cultures were set up. Six weeks after the transfer of androgenetic embryos onto the B₅ regeneration medium, they were converted into complete plants. Out of 90 androgenetic plants planted in a growth chamber, 42 plants adapted to the new conditions. All of those plants proved to be diploids in cytometric analysis.

Słowa kluczowe

PL

carrot; Daucus carota; carrot plant; plant breeding; F1 hybrid; microspore culture; androgenesis; androgenetic embryo; ploidy; regeneration

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 2
• Opis fizyczny: p.141-147,fig.,ref.

Twórcy

autor

Gorecka K

• Research Institute of Vegetable Crops, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Kowalska U

• Research Institute of Vegetable Crops, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Krzyzanowska D

• Research Institute of Vegetable Crops, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Kiszczak W

• Research Institute of Vegetable Crops, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

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