Mouse cytosolic acetyl-CoA hydrolase, a novel candidate for a key enzyme involved in fat metabolisme: cDNA cloning sequencing and functional expression

• Autorzy: Suematsu N. , Okamoto K. , Isohashi F.
• Języki publikacji: EN

Abstrakty

EN

A cytosolic acetyl-CoA hydrolase (CACH) cDNA has been isolated from mouse liver cDNA library and sequenced. Recombinant expression of the cDNA in insect cells re­sulted in overproduction of active acetyl-CoA hydrolyzing enzyme protein. The mouse CACH cDNA encoded a 556-amino-acid sequence that was 93.5% identical to rat CACH, suggesting a conserved role for this enzyme in the mammalian liver. Database searching shows no homology to other known proteins, but reveals homological cDNA sequences showing two single-nucleotide polymorphisms (SNPs) in the CACH coding region. The discovery of mouse CACH cDNA is an important step towards genetic studies on the functional analysis of this enzyme by gene-knockout and transgenic ap­proaches.

Słowa kluczowe

EN

cDNA sequence; mice; acetyl-CoA hydrolase; enzyme; enzyme activity; Spodoptera frugiperda; fat; metabolism; mouse; cDNA cloning; kidney; polymerase chain reaction; liver; functional expression

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 4
• Opis fizyczny: p.937-945,fig.

Twórcy

autor

Suematsu N.

• ST.Marianna University School of Medicine, Kanagawa 216-8511, Japan

autor

Okamoto K.

autor

Isohashi F.

Bibliografia

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