Immunosuppressory activity of the cyclodimeric peptide with RGD-sequences

• Autorzy: Szewczuk Z. , Buczek P. , Stefanowicz P. , Krajewski K. , Wieczorek Z. , Siemion I.Z.
• Języki publikacji: EN

Abstrakty

EN

Our previous studies showed that the nonapeptide fragment of HLA-DQ of the sequence H-Thr-Pro-Gln-Arg-Gly-Asp-Val-Tyr-Thr-OH, located in the β164-172 loop, strongly sup­presses the humoral and cellular immune responses, while its shorter analogs, H-Arg-Gly-Asp-Val-OH, H-Arg-Gly-Asp-Val-Tyr-OH and H-Gln-Arg-Gly-Asp-Val-Tyr-OH show only a weak stimulatory activity in respect to the humoral immunological response. These fragments contain the Arg-Gly-Asp (RGD) sequence, known for its importance for cellular association phenomena. Based on the crystal structure of HLA-DR1, we also de­signed and synthesized a cyclic analog H-Cys-Arg-Gly-Asp-Val-Tyr-Cys-OH with restricted conformation, which strongly suppresses the immune response and selectively inhibits the avβ3 integrin, suggesting that the mechanism of the immunosuppressory action of the peptide is associated with inhibition of the integrin. In this paper we present the design and synthesis of the cyclodimeric peptide,L Arg-Gly-Asp-Arg-Gly-Asp, which is also known as a selective avβ3 inhibitor. The synthesized peptide strongly suppresses both the humoral and cellular immune response. The results support our hypothesis that the immunomodulatory activity of HLA-DQ fragments may be connected with their interac­tions with some particular integrins on the cell surface.

Słowa kluczowe

EN

cyclodimeric peptide; human leucocyte antigen; peptide fragment; chemical synthesis; histocompatibility antigen; leucocyte; T cell; immune response

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 1
• Opis fizyczny: p.121-130,fig.

Twórcy

autor

Szewczuk Z.

• University of Wroclaw, F.Joliot-Curie 14, 50-383 Wroclaw, Poland

autor

Buczek P.

autor

Stefanowicz P.

autor

Krajewski K.

autor

Wieczorek Z.

autor

Siemion I.Z.

Bibliografia

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