Characterization of coagulase-negative staphylococci isolated from cases of ostitis and osteomyelitis

• Autorzy: Wilk I , Ekiel A. , Klucinski P. , Krzyszton-Russjan J. , Martirosian G.
• Języki publikacji: EN

Abstrakty

EN

Coagulase-negative staphylococci (CoNS) are often responsible for cases of chronic ostitis and osteomyelitis, especially in patients with orthopedic prosthesis/implants. The aim of this study was to characterize CoNS isolated from ambulatory patients with chronic ostitis/osteomyelitis and to compare them by PFGE (pulsed-field gel electrophoresis). Out of 263 bacterial strains isolated from wounds/sinuses of patients with chronic ostitis/osteomylitis, 41 were identified as CoNS. Twenty methicillin-resistant strains were selected for this study. Our results confirm the superior performance of cefoxitin disk test to detect methicillin resistance in heterogenous population of CoNS. High level of antibiotic resistance was observed among the studied strains: majority of CoNS were resistant to tetracycline and erythromycin and also to clindamycin and ciprofloxacin. Importantly, in 15 out of 20 studied CoNS different phenotypes of macrolides, lincosamides and streptogramin - MLS resistance was suggested. Eight strains demonstrated resistance to both erythromycin and clindamycin, suggesting constitutive MLSB phenotype. Seven remaining strains presented resistance to erythromycin and susceptibility to clindamycin with negative D-test results, suggesting the presence of macrolides and streptogramines type A efflux pump. All studied strains were sensitive to vancomycin (MIC 0.75-2.0 μg/ml), teicoplanin (MIC 0.125-8.0 μg/ml), and quinupristin/dalfopristin (MIC 0.19-1.0 μg/ml). No clonal relatedness was observed in PFGE patterns.

Słowa kluczowe

EN

osteomyelitis; antibiotic resistance; surgery; orthopedic patient; Staphylococcus aureus; microbiology; ostitis; bacterial infection

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2006
• Tom: 55
• Numer: 3
• Opis fizyczny: p.175-178,ref.

Twórcy

autor

Wilk I

• Medical University of Silesia, Katowice, Poland

autor

Ekiel A.

autor

Klucinski P.

autor

Krzyszton-Russjan J.

autor

Martirosian G.

Bibliografia

• Azap O.K., H. Arslan, F. Timurkaynak, G. Yapar, E. Oruc and U. Gagir. 2005. Incidence of inducible clindamycin resistance in staphylococci: first results from Turkey. Clin. Microbiol. Infect. 11: 577-596.
• Baron E.J. and P.R. Murray. 1999. Bacteriology pp. 246-251. In Murray PR., Baron E.J., Pfaller M.A., Tenover F.C., Yolken R.H. (eds), Manual of Clinical Microbiology, ASM Press, Washington, D.C.
• Bucher E., A. Trampuz, L. Donati and W. Zimmerli. 2000. Spondylodiscitis associated with bacteraemia due to coagulase-negative staphylococci. Eur. J. Clin. Microbiol. Infect. Dis. 19: 118-120.
• Chung M., H. de Lencastre, P. Matthews, A. Tomasz, I. Adamson, M. Aires de Sousa, et al. Multilaboratory Project Collaborators. 2000. Molecular typing of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis: comparision of results obtained in a multilaboratory effort using identical protocols and MRSA strains. Microb. Drug Resist. 6: 189-198.
• Hryniewicz W., A. Sulikowska, K. Szczypa, M. Gniadkowski and A. Skoczyhska. 2005. Recommendations for susceptibility testing to antimicrobial agents of selected bacterial species (in Polish). Post. Mikrobiol. 44: 175-192.
• Issartel B., A. Trustan, S. Lechevallier, F. Bruyere, G. Lina, B. Garin, F. Lacassin, M. Bes, F. Vandenesch and J. Etionne. 2005. Frequent carriage of Panton-Valentine leucocidin by S. aureus isolates from surgically drained abscesses. J. Clin. Microbiol. 43: 3203-3207.
• Leclercq R., A. Brisson-Noel, J. Duval and P. Courvalin. 1987. Phenotypic expression and genetic heterogeneity of lincosamide inactivation in Staphylococcus spp. Antimicrob. Agent. Chemother. 31: 1887-1891.
• Lina G., A. Quaglia, M.E. Reverdy, R. Leclercq, F. Vandenesch and J. Ettienne. 1999. Distribution of genes encoding resistance to macrolides, lincosamides, and straptogramins among staphylococci. Antimicrob. Agent Chemother. 43: 1062-1066.
• Murakami K., W. Minamide, K. Wada, E. Nakamura, H. Teraoma and S. Watanabe. 1991. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J. Clin. Microbiol. 29: 2240-2244.
• Pottumarthy S., T.R. Fritsche and R.N. Jones. 2005. Evaluation of alternative disk diffusion methods for detecting mecA-mediated oxacillin resistance in an international collection of staphylococci: Validation report from the SENTRY Antimicrobial Surveillance Program. Diagn. Microbiol. Infect. Dis. 51: 57-62.
• Rupp M.E. and G.L. Archer. 1994. Coagulase-negative staphylococci: pathogens associated with medical progress. Clin. Infect. Dis. 19: 231-243.
• Sharp S., J.A. Warren and R.B.Jr. Thomson. 2005. Cefoxitin disk diffusion screen for confirmation of oxacillin-resistant Staphylococcus aureus isolates and utility in the clinical laboratory. Diagn. Microbiol. Infect. Dis. 51: 79-71.
• Trulzsch K., H. Rinder, J. Trcek, I. Bader, U. Wilhelm and J. Hesemann. 2002. S. pettenkoferi a novel staphylococcal species isolated from clinical specimens. Diagn. Microbiol. Infect. Dis. 43: 175-182.
• von Eiff C, G. Peters and C. Hellmann. 2002. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet Infect. Dis. 2: 677-685.
• Wilk I., A. Ekiel and G. Martirosian. 2004. Staphylococci: new view on old pathogens (in Polish). Nowa Klinika 11: 718-723.