Production of urease from Helicobacter pylori in transgenic tobacco plants

• Autorzy: Brodzik R. , Koprowski H. , Yusibov V. , Sirko A.
• Języki publikacji: EN

Abstrakty

EN

H. pylori ureA and ureB genes encoding both subunits of urease were expressed transgenically in a low-alkaloid line of tobacco (LA Burley 21). Analysis of transgene expression at both the mRNA and protein levels revealed a significant increase (up to 8-fold) in ammonia concentration correlated with an amount of UreB protein detected in the leaves, and an increase (up to 2-fold) in urease activity in transformants as compared to control plants.

Słowa kluczowe

EN

protein level; ammonia; mRNA; tobacco plant; urease activity; Helicobacter pylori; transgene expression; transgenic tobacco

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2000
• Tom: 05
• Numer: 3
• Opis fizyczny: p.357-366,fig.

Twórcy

autor

Brodzik R.

• Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Koprowski H.

autor

Yusibov V.

autor

Sirko A.

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