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2001 | 45 | 2 |

Tytuł artykułu

Porcine brucellosis in Poland: problems accompanying serological surveys conducted in 1995-2000

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Analysis of problems relevant to serological surveys of pigs for brucellosis was the aim of the studies. In these investigations 74 218 sera from domestic pigs monitored for brucellosis and 3 566 sera from imported pigs were used. For comparison study 159 pig serum samples, collected from three Brucella infected herds, were tested, using following methods: RBT, ELISA, CFT, TAT and 2-ME. The obtained results indicate that serological diagnosis of brucellosis in domestic pigs did not produce major problems. Different situation was observed when some batches of imported pigs were examined. The results of serological investigations were characterised by disquietingly high proportion of samples positive in the RBT, TAT, CFT or ELISA. The analysis of documents and epidemiological inquiry concerning the pigs revealed that they originated from herds struggling with a problem of false positive serological reactions (FPSR), probably due to Yersinia enterocolitica 0:9 infection.

Wydawca

-

Rocznik

Tom

45

Numer

2

Opis fizyczny

p.153-161

Twórcy

autor
  • National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
autor

Bibliografia

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  • 3. Council Directive 64/432/EEC.
  • 4. Garin-Bastuji B., Hummel N., Gerbier G., Cau Ch., Pouillot R., Da Costa M., Fontaine J.J.: Non-specific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolitica 0:9. Vet. Microbiol., 1999, 66, 223-233.
  • 5. Gerbier G., Garin-Bastuji B., Pouillot R., Very P., Cau C., Berr V., Dufour B., Moutou F.: False positive serological reactions in bovine brucellosis: evidence of the role of Yersinia enterocolitica serotype 0:9 in a field trial. Vet. Res., 1997, 28, 375-383.
  • 6. Gourdon F., Beytout J., Reynaud A., Romaszko J.P., Perre D., Theodore P., Soubelet H., Sirot J.: Human and animal epidemic of Yersinia enterocolitica 0:9, 1989-1997, Auvergne, France. Emerg. Infect. Dis., 1999, 5, 719-721.
  • 7. Instruction no 15 of the Ministry of Agriculture, Warsaw, 1964.
  • 8. Instruction no 52 of the Ministry of Agriculture, Warsaw, 1980.
  • 9. Instruction no 55 of the Ministry of Agriculture, Warsaw, 1984.
  • 10. International Animal Health Code. OIE, Paris, 2000.
  • 11. Kittelberger R., Hilbink F., Hansen M.F., Penrose M., de Lisie G.W., Letesson J.J., Garin-Bastuji B., Searson J., Fossati C.A., Cloeckaert A., Schurig G.: Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9. I Immunoblot analysis of the antibody response to Brucella protein antigens in bovine brucellosis. Vet. Microbiol., 1995, 47, 257-270.
  • 12. Kittelberger R., Hilbink F., Hansen M.F., Ross G.P., Joyce M.A., Fenwick S., Heesemann J., Wolf-Watz H., Nielsen K.: Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9. II The use of Yersinia outer proteins for the specific detection of Yersinia enterocolitica infections in ruminants. Vet. Microbiol., 1995, 47, 271-280.
  • 13. Królak M., Stryszak A., Standardowa technika odczynu z 2-merkaptoetanolem (OME) w rozpoznawaniu brucelozy zwierząt. PIWet Puławy, 1979.
  • 14. Manual of Standards for Diagnostic Tests and Vaccines for Lists A and B Diseases of Mammals, Birds and Bees, OIE, Paris, France, 1996.
  • 15. MacMillan A. Conventional Serological Tests. In: Animal Brucellosis. Eds. Nielsen K., Duncan J.R., CRC Press, Boca Raton, USA, 1990, pp.153-197.
  • 16. Metcalf H.E., Luchsinger D.W., Ray W.C.: Brucellosis. In: Handbook of Zoonoses: Bacterial, Rickettsial, Chlamydial, and Mycotic. CRC Press, Boca Raton, USA, 1974.
  • 17. Nielsen K., Gall D., Kelly W., Hennig D., Garcia M.: Enzyme Immunoassay. Application to Diagnosis of Bovine Brucellosis, Agriculture Canada, 1992.
  • 18. Reynaud A., Delmas C., Vidon J.M.: Isolement fécal de Yersinia enterocolitica 0:9 chez plus de 50% des ruminants présentant une sérologie atypique vis-à-vis de Brucella abortus. Méd. Mal. Infect., 1993, 23, 516-519.
  • 19. Szulowski K., Pilaszek J., Truszczyński M: Zestaw ELISA do badania surowic świń w kierunku brucelozy. Medycyna Wet., 1996, 52, 513-515.
  • 20. Szulowski K., Pilaszek J., Iwaniak W., Truszczyński M.: Instrukcja dotycząca badania laboratoryjnego testem ELISA surowic świń w kierunku brucelozy. PIWet Puławy, 1999.
  • 21. Szulowski K.: Diagnosis of Brucella suis infections in pigs and hares by ELISA. Polish J. Vet. Sci., 1999, 2, 65-70.
  • 22. The Merck Veterinary Manual. A handbook of Diagnosis, Therapy, and Disease Prevention and Control for the Veterinarian. Merck&Co., Inc., Rahway, USA, 1991.
  • 23. Weynants V., Tibor A., Denoel P.A., Saegerman C., Godfroid J., Thiange P., Letesson J.J.: Infection of cattle with Yersinia enterocolitica 0:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests. Vet. Microbiol., 1996, 48, 101-112.
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Typ dokumentu

Bibliografia

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