Evidence for the hydrolysis of topical applied liposomal lipids in human stratum corneum

• Autorzy: Zellmer S
• Języki publikacji: EN

Abstrakty

EN

Liposomes were incubated with an extract of human plantar stratum corneum. The liposomal lipids were hydrolysed, if composed of soy-bean phosphatidylcholine or phosphatidylglycerol. Rigid lipids were not degraded. The temperature optimum of the hydrolysis was between 30-35°C. CaCl2 enhanced, while EDTA reduced the rate of hydrolysis, indicating that the hydrolysis is due to a phospholipase A2.

Słowa kluczowe

EN

liposome; liposomal lipid; skin; stratum corneum; man; lipid; epidermis; phospholipase; hydrolysis

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 3
• Opis fizyczny: p.885-888,fig.

Twórcy

autor

Zellmer S

• Labsoft Diagnostics AG, Robert-Franz-Ring 21, 06108 Halle, Germany

Bibliografia

• 1. Mezei, M. and Gualsekharam, V. Liposomes - a selective drug delivery system for the topical route of administration. Life Sci. 26 (1980) 1473-1474.
• 2. Cevc, G. Transfersomes, liposomes and other lipid suspensions on the skin: permeation enhancement, vesicle penetration, and transdermal delivery. Crit. Rev. Ther. Drug Carrier Syst. 13 (1996) 257-388.
• 3. Zellmer, S., Zimmermann, I., Selle, C., Sternberg, B., Pohle, W. and Lasch, J. Physicochemical characterisation of human stratum corneum lipid liposomes. Chem. Phys. Lipids 94 (1998) 97-108.
• 4. Zellmer, S., Reissig, D. and Lasch, J. Reconstructed human skin as a model for liposome-skin interaction. J. Control. Rel. 55 (1998) 271-279.
• 5. Lundstom, A. and Egelrud, T. Cell shedding from human plantar skin in vitro: evidence that two different types of protein structures are degraded by a chymotrypsin-like enzyme. Arch. Dermatol. Res. 282 (1990) 234-273.
• 6. Li-Stiles, B., Lo, H.H. and Fischer, S.M. Identification and characterization of several forms of phospholipase A2 in mouse epidermal keratinocytes. J. Lipid Res. 39 (1998) 569-583.
• 7. McCord, M., Chabot-Fletcher, M., Breton, J. and Marshall, L. A. Human keratinocytes possess an sn-2-acylhydrolase that is biochemically similar to the U937-derived 85 kDa phospholipase A2. J. Invest. Dermatol. 102 (1994) 980-986.
• 8. Mazereeuw-Hautier, J., Redoules, D., Tarroux, R., Charveron, M., Salle, J. P., Simon, M. F., Cerutti, I., Assalit, M. F. Gall, Y., Bonafe, J. L. and Chap, H. Identification of pancreatic type I secreted phospholipase A in human epidermis and ist determination by tape stripping. Br. J. Dermatol. 142 (2000) 424-431.
• 9. Mao-Qiang, M., Jain, M., Feingold, K. R. and Elias, P. M. Secretory phospholipase A2 activity is required for permeability barrier homeostasis. J. Invest. Dermatol. 106 (1996) 57-63.