Structural domains of plant nuclear DNA as a constitutive component of the topoisomerase II-DNA complex

• Autorzy: Solovyan V. T. , Andreyev I.O.
• Języki publikacji: EN

Abstrakty

EN

The treatment of agarose embedded plant nuclei by strong protein dénaturants was demonstrated to result in discrete self-fragmentation of intact nuclear DNA. The set of resultant DNA cleavage products involves two main types of DNA fragments sized about 50-100 kb and 300-500 kb, being of the same type in various eukaryotic representatives. The pattern of ordered DNA fragmentation has been shown to be similar both in intact nuclei and in histone-depleted ones thus suggesting that the observed DNA fragments represent preexisting DNA structural domains, corres­ponding to the higher levels of chromatin folding. The topoisomerase II-speeific poison teniposide (VM-26) has been shown to increase the ordered DNA cleavage while the conditions stimulating the topoisomerase II-mediated reverse reaction lead to the reassociation of the cleaved DNA domains. The data presented suggest that the nuclear DNA structural domains are involved in functioning of the topoisomerase II/DNA complex, the main property of which is its ability to mediate the cleavage/ /reassociation reactions.

Słowa kluczowe

EN

chromatin; cleavage; DNA complex; topoisomerase II; plant

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.201-204,fig.

Twórcy

autor

Solovyan V. T.

• Academy of Sciences of Ukraine, Kiev 252627, Ukraine

autor

Andreyev I.O.

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