Wystepowanie wirusa TT u chorych z zapaleniem watroby typu B, C i o nieustalonej etiologii

• Autorzy: Liwen I. , Januszkiewicz-Lewandowska D. , Rembowska J. , Pernak M. , Nowicka-Kujawska K. , Nowak J.
• Języki publikacji: PL

Abstrakty

PL

W pracy przedstawiono analizę częstości występowania wirusa TT w surowicy krwi chorych z przewlekłym zapaleniem wątroby typu B, C i o nieustalonej etiologii, a także w kontrolnej grupie krwiodawców. Dodatkowo zbadano częstość infekcji TTV u chorych szczególnie narażonych na zakażenia wirusami przenoszonymi drogą parenteralną. Dokonano także, w wybranych przypadkach, analizy sekwencji nukleotydów w genomie wirusa TT.

EN

Discovery of TT virus in 1997 gave raise to intensive subsequent studies to lern about its structure, features and, what is the most important, about its role in pathogenesis of liver disease. The aim of the work was to analyze prevalence of TTV DNA in patients with diagnosed hepatitis B, C, that of unknown etiology and in healthy blood donors as well. Additionally the divergence of TTV sequence was estimated in selected cases. TTV DNA was detected by PCR technique using specific oligonucleotide primers for coding regions. TT virus has been detected in 25,6% (32/125) HBsAg positive patients and in 23.9% (51/213) HCV infected patients. In healthy blood donors the frequency of TTV was 24.3% (34/140) similarly to that found in HCV and HBV infected patients. The frequency of TTV DNA among patients with hepatitis of unknow etiology was 9,1%. This result was statistically significant lower than in the other groups. When detected sequences have been compared to these from NCBI base the homology result was 71% to 95%, and among different patients and groups of patients identity was 46% to 73%. On the basis of the obtained results it can be concluded that it is very unlikely that TTV coinfection plays any significant role in HCV or HBV infection. The hypothetical role of TTV infection in the etiopathogenesis of cryptogenic chronic hepatitis has not been confirmed. The results obtained in the small group of patients with hepatitis of unknown etiology are not conclusive and should be taken with some precaution. The final conclusion is the TTV coinfection does not to contribute to the liver pathology. The divergence of TTV sequences may explain the various frequency of TTV viremia reported by other authors.

Słowa kluczowe

PL

surowica krwi; zapalenie watroby typu B; diagnostyka medyczna; wystepowanie; choroby czlowieka; etiologia; zapalenie watroby typu C; wirusy TT

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2002
• Tom: 54
• Numer: 2
• Opis fizyczny: s.151-155,tab.,bibliogr.

Twórcy

autor

Liwen I.

• Zaklad Genetyki Czlowieka PAN, 60-479 Poznan, ul.Strzeszynska 32

autor

Januszkiewicz-Lewandowska D.

autor

Rembowska J.

autor

Pernak M.

autor

Nowicka-Kujawska K.

autor

Nowak J.

Bibliografia

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• 2. Erker JC, Leary TP, Desai SM i inni. Analyses of TT virus full-lenght genomic sequences. J Virol 1999; 80: 1743-50.
• 3. Gimenez-Barcons M, Forns X, Ampurdanes S i inni. Infection with a novel human DNA virus (TTV) Has no pathogenic significance in patients with liver diseases. J Hepatol 1999; 30: 1028-34.
• 4. Ikeda H,Takasu M, Inoue K i inni. Infection with an uneveloped DNA virus (TTV) in patients with acute or chronic liver disease of unknown etiology and in those positive for hepatitis C virus RNA. J Hepatol 1999; 30: 205-12.
• 5. Kato T, Mizokami M, Orito E i inni. High prevalence of TT virus infection in Japanese patients with liver diseases and in blood donors. J Hepatol 1999; 31: 221-27.
• 6. Nishizawa T, Okamoto H, Konishi K i inni. A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochem Biophys Res Commun 1997; 241: 92-97.
• 7. Takahashi K, Hoshino H, Ohta Y i inni. Very high prevalence of TT virus (TTV) infection in general population of Japan revealed by a new set of PCR primers. Hepatol Res 1998; 12: 233-39.
• 8. Tangkijvanich P, Theamboonlers A, Hirsch P i inni: TT virus infection in chronic liver disease. Hepatogastroenterology 1999; 46; 1053-58.