Immunisation of carp [Cyprinus carpio L.] against motile Aeromonas

• Autorzy: Kozinska A , Antychowicz J.
• Języki publikacji: EN

Abstrakty

EN

The purpose of the study was to evaluate an optimum dose of Aeromonas hydrophila and Aeromonas sobria antigens for the vacccination of carp (Cyprinus carpio L.) and to compare the effectiveness of the vaccination with the antigens inactivated with formalin or thermally and given intraperitoneally (ip) or by immersion (imm). The dose was evaluated with the use of formalin antigens. Doses ranging from 3 x 10⁴ to 6 x 10⁸ cells were given by injection and doses of 3 x 10⁵ to 6 x 10⁶ cells/mL of water were administered in bath. Experiments were conducted at 12°C, 16°C, and 23°C ± 1°C . To compare the effectiveness of the antigens inactivated with formalin and thermally the two doses 3 x 10⁸ cells (ip) and 5- 6 x 10⁷cells/mL (imm) were used. The effect of immunisation was evaluated with challenge tests. The relative percentage of health (RPH) and relative percentage of survival (RPS) of fish were calculated. Doses of 3 x 10⁸ cells, and 5-6 x 10⁷ cells/mL given ip and imm, respectively, were considered the most effective irrespective of the water temperature. No marked differences were found between the administration of the antigen by injection and immersion. Fish manifested a significantly higher level of immunity after the administration of formalin antigen in comparison to that following the administration of the antigen inactivated thermally.

Słowa kluczowe

EN

Aeromonas sobria; Aeromonas hydrophila; carp; Cyprinus carpio; immunization; Aeromonas

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2000
• Tom: 44
• Numer: 1
• Opis fizyczny: p.53-58

Twórcy

autor

Kozinska A

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Antychowicz J.

Bibliografia

• 1. Austin B., Austin D.A.: Bacterial fish pathogens. Ellis Horwood Limited, New York, 1987, p. 172.
• 2. Ellis A.E.: Fish vaccination. Academic Press, London, 1988.
• 3. Kozińska A.: Wskaźniki patogenności Aeromonas hydrophila, Aeromonas caviae i Aeromonas sobria. Praca dokt. PIWet, Puławy, 1996.
• 4. Krausanagar I., Rosalind G., Krausanagar I.: J. Fish Dis. 14, 413, 1991.
• 5. Lamers C.H.J., De Haas M.J.M., Van Muiswinkel W.B.: Develop. Comp. Immunol. 9, 65, 1985.
• 6. Lamers C.H.J., De Haas M.J.M., Van Muiswinkel W.B.: J. Fish Dis. 8, 253, 1985.
• 7. Newman S.G.: Ann. Rev. Fish Diseases p.145, 1993.
• 8. Popoff M.: Genus III Aeromonas. W: Krieg NR, Holt J.G.: Bergey's Manual of Systemetic Bacteriology t.1. Wiliams and Wilkins, Baltimore, 1984, p.545.
• 9. Santos Y., Toranzo A.E., Barja J.L., Nieto T.P., Villa G.: Infect. Immunol. 56, 3285, 1988.
• 10. Stevenson R.M.W.: Vaccination against Aeromonas hydrophila. W: Ellis A.E.: Fish vaccination. Academic Press, London, 1988.