ISSR analysis of chosen Gleditsia accessions obtained from Polish collections

• Autorzy: Smolik M , Kubus M.
• Języki publikacji: EN

Abstrakty

EN

Species of Gleditsia show considerable morphological variability that makes them difficult to distinguish using either vegetative or floral characters. Honeylocusts, especially the thornless cultivars, are popular ornamental, shade, street, attractive landscape trees. In this study the ISSR technique was used to evaluate the range of genetic variability between seven genotypes of Gleditsia cultivated in Polish dendrological collections [Gleditsia caspica Desf., Gleditsia japonica Miq., Gleditsia japonica Miq. var. korainensis (= G. korainensis Nakai), Gleditsia triacanthos L., Gleditsia triacanthos L. (bulk), Gleditsia triacanthos f. inermis (L.) Zabel (bulk). Forty ISSR primers were tested and 18 were selected for their ability to produce clear and reproducible patterns of multiple bands.A total of 177 loci of 260-2600 bp were amplified, of which 89 (50%) were polymorphic, 14 (8%) monomorphic and 74 (42%) were accession-specific. Accession-specific ISSR loci were obtained for all of the seven accessions tested. A dendrogram generated using the UPGMA, based on a similarity measure of total character difference, showed that the Gleditsia accessions were clustered into two main groups (‘a’ and ‘b’). The first grup –‘a’– included: Gleditsia triacanthos L., Gleditsia triacanthos L. (bulk) and Gleditsia triacanthos f. inermis (L.) Zabel (similarity 0.61–0.75), the second –‘b’– included 2 species: Gleditsia japonica and Gleditsia japonica var. korainensis (similarity 0.43). Analysis of the phylogenetic similarity dendrogram has shown wide range of diversity between studied accessions. The clustering pattern obtained in our experiment was in agreement with the data based on morphological, allozyme and ITS analysis.

Słowa kluczowe

EN

ISSR technique; Gleditsia; Polish collection; genetic variability; fingerprinting; dendrology; Poland

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2009
• Tom: 62
• Opis fizyczny: p.63-70,fig.,ref.

Twórcy

autor

Smolik M

• West Pomeranian University of Technology, Janosika 8, 71-424 Szczecin, Poland

autor

Kubus M.

Bibliografia

• Blair R.M. 1990. Gleditsia triacanthos L. Honeylocust. Silvics of North American Trees, vol. 2 Hardwoods. USDA Handb. 654: 358–364.
• Bojarczuk T., Zieliński J. 1980. Kolumnowa forma glediczji (Gleditsia L.) w Arboretum Kórnickim. Arbororetum Kórnickie 15: 325–328.
• Condit R., Hubbel S.P., Foster R. B. 1994. Density dependence in two understory tree spiecies in a Neotropical forest. Ecology 75: 671–680.
• Czekalski M., Danielewicz W. 1997. Glediczja trójcierniowa (Gleditsia triacanthos L.) w Polsce. Rocznik Dendrologiczny 45: 5–34.
• Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791.
• Fowells H.A. 1965. Silvics of forest trees of the United States. Agriculture Handbook. 271: 197–201.
• Iruela M., Rubio J., Cubero J.I., Gil J., Millán T. 2002. Phylogenetic analysis in the genus Cicer and cultivated chickpea using RAPD and ISSR markers. Theoretical Applied Genetics 104: 643–651.
• Krüssmann G. 1960. Handbuch der Laubgehölze. Bd 1. Paul Parey. Berlin und Hamburg: 474–477.
• Kubus M., 1999. Fenologia glediczji trójcierniowej Gleditsia triacanthos L. na różnych stanowiskach w Szczecinie. Folia Universitas Agriculturae Stetinensis 198: 81–157.
• Michener C.D. 1986. Phenotypic instability in Gleditsia triacanthos (Fabaceae). Brittonia 38: 360–361.
• Mondal T.K. 2002. Assessment of genetic diversity of tea (Camellia sinesis (L.) O. Kuntze) by inter simple sequence repeat polymerase chain reaction. Euphytica. 128: 307–315.
• Nei M., Li W.H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proccedings of the National Academy of Science USA 76: 5269–5273.
• Paclt J. 1982. Gleditsia caspica, not a distinct species (Leguminosae). Taxon 31: 336–339.
• Rajesh P.N., Sant V.J., Gupta V.S., Muehlbauer F.J., Ranjekar P.K. 2002. Genetic relationships among annual and perennial wild species of Cicer using inter simple sequence repeat (ISSR) polymorphism. Euphytica 129: 15–23.
• Rehder A., 1960. Manual of cultivated trees and shrubs hardy in North America. New York the Macmillan Company pp. 85–487.
• Rzepka-Plevneš D., Smolik M., Tańska K. 2006. Genetic similarity of chosenSyringa species determined by the ISSR-PCR technique. Dendrobiology 56: 61–67.
• Rzepka-Plevneš D., Smolik M., Drzewiecka K. 2007. Genetic variation of some botanical and cultivar varieties of Acer sp. Journal of Food Agriculture and Environment 5: 481–485.
• Schnabel A., Hamrick J.L. 1990. Organization of genetic diversity within and among populations of Gleditsia triacanthos (Leguminosae). American Journal of Botany 77: 1060–1069.
• Schnabel A., Krutovskii K.V. 2004. Conservation genetics and evolutionary history of Gleditsia caspica: Inferences from allozyme diversity in populations from Azerbaijan. Conservation Genetics 5: 195–204.
• Schnabel A., McDonel P.E, Wendel J.F. 2003. Phylogenetic relationship in Gleditsia (Leguminosae) based on ITS sequences. American Journal of Botany 90: 310–320.
• Schnabel A., Wendel J.F. 1998. Cladistic biogeography of Gleditsia (Leguminosae) based on ndhF and rpl16 chloroplast gene sequences. American Journal of Botany 85: 1753–1765.
• Seneta W. 1996. Drzewa i krzewy liściaste. Wydaw. Nauk. PWN, T. III, Daboecia-Hyssopus: 250–257.
• Sudupak M.A. 2004. Inter and intra-species Inter Simple Sequence Repeat (ISSR) variations in the genus Cicer. Euphytica 135: 229–238.
• Tsumura Y., Ohba K., Strauss S.H. 1996. Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica).Theoretical Applied Genetics 92: 40–45.
• Tumiłowicz J. 2005. Dendrological collections of the Warsaw Agricultural University Arboretum in Rogów – results of many years cultivation of selected species. Part III. Broadleaves (Lardizabalaceae – Verbenaceae). Rocznik Dendrologiczny 53: 89–110.
• Van de Peer Y., De Wachter R. 1994. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Computer Applied Bioscience 10: 569–570.
• Van der Nest M.A., Steenkamp E.T., Wingfield B.D., Wingfield M.J. 2000. Development of simple sequence repeat (SSR) markers in Eucalyptus from amplified inter-simple sequence repeats (ISSR). Plant Breeding 119: 433–436.
• Vijayan K., Chatterjee S.N. 2003. ISSR profiling of Indian cultivars of mulberry (Morus spp.) and its relevance to breeding programs. Euphytica 131: 53–63.
• Wolko B., Irzykowska L., Święcicki W.K. 1999. AFLP i SSR – systemy markerowe przydatne w hodowli roślin. Postępy Nauk Rolniczych 46: 59–71.
• Ziętkiewicz E., Rafalski A. and Labuda D. 1994. Genome fingerprinting by simple sequence repeat (SSR) – anchored polymerase chain reaction amplification. Genomics 20: 176–183.