A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions

• Autorzy: Kawalec M. , Borsuk P. , Piechula S. , Stepien P.P.
• Języki publikacji: EN

Abstrakty

EN

A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate.

Słowa kluczowe

EN

Antarctic; restriction endonuclease; phosphate ion; phosphate inhibition; bacteria

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1997
• Tom: 44
• Numer: 4
• Opis fizyczny: p.849-852,fig.

Twórcy

autor

Kawalec M.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Borsuk P.

autor

Piechula S.

autor

Stepien P.P.

Bibliografia

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• Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning. Cold Spring Harbour Laboratory, N.Y.