Detection of the metabolic hormone residues in the urine and muscles of food - producing animals by HPTLC with application of extraction into a solid phase

• Autorzy: Wozniak B , Wojton B.
• Języki publikacji: EN

Abstrakty

EN

A simple method for determination of 8 anabolie hormone residues in the muscles and urine of slaughter animals was elaborated. Forty cattle muscle samples and 30 urine samples fortified with diethyl stilbestrol, dienstrol, hexastrol, zeranol, trenbolone, trenbolone acetate, 19-nortesterone, and medroxyprogesterone acetale at concentrations of 1 to 20 ng/g (ppb) were examined. The hormones were extracted with methanol and the methanol phase was degreased with n-hexane and extracted with diethyl ether. The ether layer was washed with carbonate buffer and water evaporated to dryness. The residues were dissolved in acetate buffer and cleaned up by extraction into the solid phase on C18 columns. In urine, the hormones were extracted with diethyl ether followed by the procedure corresponding to that for muscles. After evaporating and dissolving in acetone the samples were analysed by two-dimentional HPTLC methods. The chromatograms were developed with 5% sulphuric acid in ethanol. The detection limit of the hormones examined on the HPTLC plate was 5-20 ng, and in the tissue 1 ppb for trenbolone and 2 ppb for the remaining compounds.

Słowa kluczowe

EN

extraction; anabolic hormone; muscle; measurement; food; purification; thin-layer chromatography; urine; high performance liquid chromatography; hormone

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1996
• Tom: 40
• Numer: 1
• Opis fizyczny: p.55-59

Twórcy

autor

Wozniak B

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Wojton B.

Bibliografia

• 1. Medycyna Wet. 9, 51, 1995.
• 2. Bettin C., Furst P.: Fleischwirtschaft 69, 1033, 1989.
• 3. De Brabander H.F., Venhee P., Van Hoye S., Verbeke R.: J. Planar Chromatogr. 2, 33, 1989.
• 4. Smets F.,Vandewalle M.: Z. Lebensm. Unters. Forsch. 175, 29, 1982.
• 5. Smets F., Vandewalle M.: Z. Lebensm. Unters. Forsch. 178, 38, 1984.
• 6. Medina M.B., Sherman J.T.: Food Additives and Contaminants 3, 263, 1986.
• 7. Jansen E.H.J.M., Both-Miedema R., Van Blitterswijk H., Stephany R.W.: J. Chromatogr. 299, 450, 1984.
• 8. Gaspar P., Maghuin-Rogister G.: J. Chromatogr. 328, 413, 1985.
• 9. Daeseleire E., Vanoosthuyze K., Van Peteghem C.: J. Chromatogr. 674, 247, 1994.
• 10. Hooijerink D., Schilt R., Van Bennekom E., Brouwer B.: Analyst 119, 2617, 1994.
• 11. Verbeke R.: J. Chromatogr. 177, 69, 1979.
• 12. Commission of the Europ. Comm.: Residues in food-producing animals, their products: Reference materials and methods. Heitzman R.J. United Kingdom, 1992.
• 13. De Brabander H.F., Pottie G.: J. Planar Chromatogr. 1, 369, 1988.