Measurement of milk D-3-hydroxybutyrate with a simple UV spectrophotometer method: an alternative assay method

• Autorzy: Zhang Z.G. , Liu G. W. , Li X. B. , Li Y. F. , Guo C. M. , Gao L. , Wang H. B. , Xu S. W. , Wang Z.
• Języki publikacji: EN

Abstrakty

EN

The measurement of D-3-hydroxybutyrate (D-BHBA) in milk samples is an important tool for diagnosis of subclinical/clinical ketosis in dairy cows. We describe a simple UV spectrophotometric method for measuring the concentration of D-BHBA in milk of dairy cows. From two herds, 119 milk samples were taken from dairy cows. The standard-curve equation was y = 0.2582x + 0.0269 (R² = 0.9967). The assay was highly specific with a minimum detection limit of 0.01 mmol/L and measuring range of up to 5 mmol/L. The recovery was between 99.35% and 100.22% and repeatability was 99.8%. The comparison between the spectrophotometric method and the fluorometric method revealed a close correlation (r = 0.9939). These results show that the spectrophotometric method can be successfully used as an alternative method to measure D-BHBA content in milk.

Słowa kluczowe

EN

measurement; milk; D-3-hydroxybutyrate; simple ultraviolet spectrophotometry; dairy cow; cow; ketosis; ketone level; spectrophotometric method

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2009
• Tom: 12
• Numer: 4
• Opis fizyczny: p.563-565,fig.,ref.

Twórcy

autor

Zhang Z.G.

• College of Animal Science and Veterinary Medicine, Jilin University, 5333 of Xi'an Road, Changchun, Jilin, 130062, China

autor

Liu G. W.

autor

Li X. B.

autor

Li Y. F.

autor

Guo C. M.

autor

Gao L.

autor

Wang H. B.

autor

Xu S. W.

autor

Wang Z.