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2012 | 34 | 6 |

Tytuł artykułu

Role of vacuolar invertase in regulating Arabidopsis stomatal opening

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Guard cells sense and integrate environmental signals to modulate stomatal aperture in response to diverse conditions. In this study, the effect of vacuolar invertase on Arabidopsis stomatal opening was investigated. The technology of enzyme activity detection in situ was used to show that the vacuolar invertase activity was much higher in guard cells than in other epidermal cells. The stomatal aperture of T-DNA insertion mutant in At1g12240 (inv-7) was significantly lower than that in wild-type plants. Increased stomatal aperture was observed in the transgenic Arabidopsis overexpressing cotton vacuolar invertase gene. These results indicated that Arabidopsis stomatal aperture was correlated with vacuolar invertase, and that vacuolar invertase may play an important role in regulating Arabidopsis stomatal opening.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

34

Numer

6

Opis fizyczny

p.2449-2452,fig.,ref.

Twórcy

autor
  • School of Ecological Technology and Engineering, Shanghai Institute of Technology, 100 Haiquan Rd, 201418 Shanghai, People's Republic of China

Bibliografia

  • Gonzalez MC, Roitsch T, Cejudo FJ (2005) Circadian and developmental regulation of vacuolar invertase expression in petioles of sugar beet plants. Planta 222:386–395
  • Hirose T, Takano M, Terao T (2002) Cell wall invertase in developing rice caryopsis: molecular cloning of OsCIN1 and analysis of its expression in relation to its role in grain filling. Plant Cell Physiol 43:452–459
  • Kang Y, Outlaw WH Jr, Andersen PC, Fiore GB (2007a) Guard-cell apoplastic sucrose concentration–a link between leaf photosynthesis and stomatal aperture size in the apoplastic phloem loader Vicia faba L. Plant Cell Environ 30:551–558
  • Kang Y, Outlaw WH Jr, Fiore GB, Riddle KA (2007b) Guard cell apoplastic photosynthate accumulation corresponds to a phloemloading mechanism. J Exp Bot 58:4061–4070
  • Lu P, Outlaw WH Jr, Smith BG, Freed GA (1997) A new mechanism for the regulation of stomatal aperture size in intact leaves (accumulation of mesophyll-derived sucrose in the guard-cell wall of Vicia faba). Plant Physiol 114:109–118
  • Reddy AR, Das VSR (1986) Stomatal movement and sucrose uptake by guard cell protoplasts of Commelina benghalensis L. Plant Cell Physiol 27:1565–1570
  • Roitsch T, Gonzalez MC (2004) Function and regulation of plant invertases: sweet sensations. Trends Plant Sci 9:606–613
  • Sergeeva LI, Vreugdenhil D (2002) In situ staining of activities of enzymes involved in carbohydrate metabolism in plant tissues. J Exp Bot 53:361–370
  • Sergeeva LI, Keurentjes JJ, Bentsink L, Vonk J, van der Plas LH, Koornneef M, Vreugdenhil D (2006) Vacuolar invertase regulates elongation of Arabidopsis thaliana roots as revealed by QTL and mutant analysis. Proc Natl Acad Sci USA 103:2994–2999
  • Talbott LD, Zeiger E (1996) Central roles for potassium and sucrose in guard-cell osmoregulation. Plant Physiol 111:1051–1057
  • Talbott LD, Zeiger E (1998) The role of sucrose in guard cell osmoregulation. J Exp Bot 49:329–337
  • Wang L, Li XR, Lian H, Ni DA, He YK, Chen XY, Ruan YL (2010) Evidence that high activity of vacuolar invertase is required for cotton fiber and Arabidopsis root elongation through osmotic dependent and independent pathways, respectively. Plant Physiol 154:744–756

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Bibliografia

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