Essential oil and plant extract of oregano as agents influencing the virulence factors of Candida albicans

• Autorzy: Vaczi P. , Proskovcova M. , Conkova E. , Marcincakova D. , Backorova M. , Harcarova M.
• Języki publikacji: EN

Abstrakty

EN

Candida albicans, a polymorphic yeast, is a physiological component of the human and animal commensal microbiome. It is an etiological factor of candidiasis, which is treated by azole antifungals. Growing resistance to azoles is a reason to look for other alternative treatment options. The pharmacotherapeutic use of plant extracts and essential oils has become increasingly important. In our experiment, C. albicans showed susceptibility to four observed plant extracts and essential oils from peppermint (Mentha piperita), thyme (Thymus vulgaris), sage (Salvia officinalis), and oregano (Origanum vulgare). Oregano plant extract and essential oil showed the highest antifungal activity, at MIC values of 4.9 mg/mL and 0.4 mg/mL respectively. Therefore, it was subjected to further research on the influence of virulence factors – biofilm formation, extracellular phospholipase production and germ tube formation. Oregano plant extract and essential oil showed an inhibitory effect on the observed C. albicans virulence factors at relatively low concentrations. The extract inhibited the adherence of cells at MIC 12.5 mg/mL and essential oil at MIC 0.25 mg/mL. Degradation of the formed biofilm was detected at MIC 14.1 mg/mL for plant extract and at MIC 0.4 mg/mL for essential oil. Extracellular phospholipase production was most effectively inhibited by the essential oil. In particular, the number of isolates with intensive extracellular phospholipase production decreased significantly.Of the 12 isolates intensively producing extracellular phospholipase, only 1 isolate (4.5%) retained intense production. Essential oil caused up to a 100 % reduction in germ tubes formation and plant extract reduced their formation depending on the concentration as follows: 2.6% (0.8 mg/mL), 21.2 % (6.25 mg/mL), and 64.5 % (12.5 mg/mL) compared to the control.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2024
• Tom: 27
• Numer: 1
• Opis fizyczny: p.25-33,fig.,ref.

Twórcy

autor

Vaczi P.

• Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, Košice, 041 81, Slovakia

autor

Proskovcova M.

• Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, Košice, 041 81, Slovakia

autor

Conkova E.

• Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, Košice, 041 81, Slovakia

autor

Marcincakova D.

• Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, Košice, 041 81, Slovakia

autor

Backorova M.

• Department of Pharmaceutical Technology, Pharmacognosy and Botany, University of Veterinary Medicine and Pharmacy, Komenského 73, Košice, 041 81, Slovakia

autor

Harcarova M.

• Department of Animal Nutrition and Husbandry, University of Veterinary Medicine and Pharmacy, Komenského 73, Košice, 041 81, Slovakia

Identyfikatory

DOI

10.24425/pjvs.2024.149330