Proanthocyanidins in Rhodiola kirilowii and Rhodiola rosea callus tissues and transformed roots - determination with UPLC-MS/MS method

• Autorzy: Gryszczynska A. , Krajewska-Patan A. , Dreger M. , Buchwald W. , Pietrosiuk A. , Zych M. , Karasiewicz M. , Bogacz A. , Kujawski R. , Furmanowa M. , Czerny B. , Mielcarek S. , Gazda P. , Mrozikiewicz P. M.

Warianty tytułu

PL

Proantocyjanidyny w tkankach kalusowych i w transformowanych korzeniach Rhodiola kirilowii i Rhodiola rosea - oznaczenie za pomocą metody UPLC-MS/MS

• Języki publikacji: EN

Abstrakty

EN

Several species of Rhodiola genus (Crassulaceae family) like Rhodiola kirilowii and Rhodiola rosea are used in official or traditional medicine. The aim of this study was to determine qualitative and quantitative content of proanthocyanidins using ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) in the callus tissues and in the transformed roots (infected by Agrobacterium rhizogenes LBA 9402 strain) of R. kirilowii and R. rosea. This validated assay allows to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG). Our results concerning the material from in vitro cultivation of R. kirilowii and R. rosea indicate that R. rosea callus can be a better source of catechin when compared with other tested materials, especially when the content of (-)-gallate epigallocatechin is taken under consideration (3.429 mg/100 g of dry powdered material). The application of UPLC MS/MS method allowed to determine the content of proanthocyanidins in tested samples with satisfactory precision and can be used in the phytochemical investigations of Rhodiola sp. in vitro cultivated tissues.

PL

Niektóre gatunki z rodzaju Rhodiola (rodzina Crassulaceae), jak Rhodiola kirilowii i Rhodiola rosea, są stosowane w medycynie oficjalnej lub ludowej. Celem przedstawionych badań było oznaczenie jakościowe i ilościowe proantocyjanidyn w tkankach kalusowych i w transformowanych (za pomocą szczepu Agrobacterium rhizogenes LBA 9402) korzeniach Rhodiola kirilowii i Rhodiola rosea przy zastosowaniu metodyki wykorzystującej ultrasprawny chromatograf cieczowy sprzężony z tandemowym spektrometrem mas (metoda UPLC MS/MS). Ta zawalidowana metodyka pozwoliła na określenie stężeń pięciu flawan-3-oli: (+)-katechiny, (-)-epikatechiny, (-)-epigalokatechiny, galusanu (-)-epikatechiny (ECG) oraz galusanu (-)-epigalokatechiny (EGCG). Przedstawione w pracy wyniki dotyczące materiału pochodzącego z kultur in vitro R. kirilowii i R. rosea wskazują, że kalus R. rosea jest lepszym źródłem katechin w porównaniu do pozostałych badanych surowców, szczególnie, gdy bierze się pod uwagę zawartość galusanu epigalokatechiny (3.429 mg/100 g suchego sproszkowanego surowca). Zastosowanie opracowanej metodyki z wykorzystaniem ultrasprawnego chromatografu cieczowego sprzężonego z tandemowym spektrometrem mas pozwoliło z zadawalającą precyzją oznaczyć zawartości proantocyjanidyn w analizowanych próbkach. Metoda ta może być stosowana w fitochemicznych badaniach hodowanych in vitro tkanek rodzaju Rhodiola.

Słowa kluczowe

EN

proanthocyanidin; Rhodiola kirilowii; Rhodiola rosea; callus tissue; transformed root; root; determination; ultra performance liquid chromatography-tandem mass spectrometric method

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2012
• Tom: 58
• Numer: 4
• Opis fizyczny: p.51-61,fig.,ref.

Twórcy

autor

Gryszczynska A.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Krajewska-Patan A.

• Department of Pharmacology and Experimental Biology, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Dreger M.

• Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71 B, 60-630 Poznan, Poland

autor

Buchwald W.

• Team of Botany and Agriculture of Medicinal Plants, Department of Botany, Breeding and Agricultural Technology, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska/Poznan, Poland

autor

Pietrosiuk A.

• Department of Biology and Pharmaceutical Botany, Warsaw Medical University, Banacha 1, 02-097 Warsaw, Poland

autor

Zych M.

• Department of Biology and Pharmaceutical Botany, Warsaw Medical University, Banacha 1, 02-097 Warsaw, Poland

autor

Karasiewicz M.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Bogacz A.

• Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Kujawski R.

• Department of Pharmacology and Experimental Biology, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Furmanowa M.

• Department of Biology and Pharmaceutical Botany, Warsaw Medical University, Banacha 1, 02-097 Warsaw, Poland

autor

Czerny B.

• Department of General Pharmacology and Pharmaeconomics, Pomeranian Medical University, Zolnierska 48, 70-204 Szczecin, Poland

autor

Mielcarek S.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Gazda P.

• Clinic of Pediatric Surgery and Oncology, Specialistic Hospital Named After Professor A. Sokolowski of Pomeranian Medical University, Unii Lubelsiej 1, 71-242 Szczecin, Poland

autor

Mrozikiewicz P. M.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

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