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2015 | 57 | 1 |

Tytuł artykułu

Hypoxia-induced programmed cell death in root-tip meristematic cells of Triticum aestivum L.

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Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In this study, wheat (Triticum aestivum L.) roots were treated with hypoxic water. The staining of cell preparations with DAPI revealed morphological changes of the cells such as nuclear condensation, deformation and fragmentation. Under TEM, cellular membrane shrinkage and breakage, chromatin condensation and apoptotic- like bodies were displayed. The number of mitochondria increased dramatically; their cristae were damaged; the interior became a cavitation and only some flocculent materials were distributed. Indirect immunofluorescence staining indicated that cytochrome C diffused from mitochondria to nucleoplasm and cytoplasm. TUNEL positive nuclei indicated double strand breaks of DNA. DAB staining was used for the identification of hydrogen peroxide and examination showed that the longer the treating time, the darker the staining of the meristematic zones of the roots which suggested the increased accumulation of these Reactive Oxygen Species (ROS). The elevation of hydrogen peroxide production was paralleled with the increase of SOD and POD activities. A negative correlation between the exposure time under hypoxia and the contents of soluble proteins was found. No obvious effect of hypoxia on MDA was established. The obtained results demonstrate that hypoxia causes programmed cell death in the root-tip meristematic cells of Triticum aestivum L. which is most probably attributed to the accumulation of large amounts of ROS.

Wydawca

-

Rocznik

Tom

57

Numer

1

Opis fizyczny

p.51-61,fig.,ref.

Twórcy

autor
  • College of Life Sciences, Capital Normal University, No.105, Xi San Huan Bei Lu, Beijing 100048, China
autor
  • College of Life Sciences, Capital Normal University, No.105, Xi San Huan Bei Lu, Beijing 100048, China

Bibliografia

  • ARMSTRONG W. 1979. Aeration in higher plants. Advances in Botanical Research 7: 225–331.
  • BROKER LE, KRUYT FA, and GIACCONE G. 2005. Cell death independent of caspases: a review. Clinical Cancer Research11: 3155–3162.
  • BRAILSFORD RW, VOESENEK LACJ, BLOM CWPM, SMITH AR, HALL MA, and JACKSON MB. 1993. Enhanced ethylene productionby primary roots of Zea mays L. in response to subambientpartia1 pressures of oxygen. Plant, Cell andEnvironment 16: 1071–1080.
  • COLMER TD. 2003. Long-distance transport of gases in plants: a perspective on internal aeration and radial oxygen loss from roots. Plant, Cell and Environment 26: 17–36.
  • DANON A, DELORME V, MAILHAC N, and GALLOIS P. 2000. Plant programmed cell death: A common way to die. Plant Physiology and Biochemistry 38: 647–655.
  • DANON A, and GALLOIS P. 1998. UV-C radiation induces apoptotic- like changes in Arabidopsis thaliana. FEBS Letters437: 131–136.
  • DINAKAR C, ABHAYPRATAP V, YEARLA SR, RAGHAVENDRA AS, and PADMASREE K. 2010. Importance of ROS and antioxidantsystem during the beneficial interactions of mitochondrialmetabolism with photosynthetic carbon assimilation.Planta 231: 461–474.
  • DREW MC. 1997. Oxygen deficiency and root metabolism: injury and acclimation under hypoxia and anoxia. Annual Review of Plant Physiology and Plant Molecular Biology 48: 223–250.
  • DREW MC, HE CJ, and MORGAN PW. 2000. Programmed cell death and aerenchyma formation in roots. Trends in Plant Science 5: 123–127.
  • EVANS DE. 2003. Aerenchyma formation. New Phytologist 161: 35–49.
  • GREENBERG JT. 1996. Programmed cell death: a way of life for plants. Proceedings of the National Academy of Sciences U.S.A. 93: 12094–12097.
  • GREIGER AE, and VAN DER WALL E. 2004. The role of hypoxia inducible factor 1 (HIF1) in hypoxia induced apoptosis. Journal of Clinical Pathology 57: 1009–1014.
  • GUNAWARDENA AH, PEARCE DM, JACKSON MB, HAWES CR, and EVANS DE. 2001. Characterisation of programmed cell death during aerenchyma formation induced by ethylene or hypoxia in roots of maize (Zea mays L.). Planta 212: 205–214.
  • HE CJ, MORGAN PW, and DREW MC. 1996. Transduction of an ethylene signal is required for cell death and lysis inthe root cortex of maize during aerenchyma formationinduced by hypoxia. Plant Physiology 112: 463–472.
  • HEATH RL, and PACKER L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acidperoxidation. Archives of Biochemistry and Biophysics125: 189–198.
  • JOSHI R, and KUMAR P. 2012. Lysigenous aerenchyma formation involves non-apoptotic programmed cell death in rice (Oryza sativa L.) roots. Physiology and Molecular Biology of Plants 18: 1–9.
  • KATSUHARA M, and KAWASAKI T. 1996. Salt stress induced nuclear and DNA degradation in meristematic cells of barley roots. Plant Cell Physiology 37: 169–173.
  • KERR JFR, WYLLIE AH, and CURRIE AR. 1972. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. British Journal of Cancer 26: 239–257.
  • KRISHNAMURTHY KV, KRISHNARAI R, CHOZHAVENDAN R, and CHRISTOPHER FS. 2000. The program of cell death in plants and animals – A comparison. Current Science 79: 1169–1181.
  • MUNNE-BOSCH S, and ALEGRE L. 2004. Die and let live: leaf senescence contributes to plant survival under drought stress. Functional Plant Biology 31: 203–216.
  • NEILL SJ, DESIKAN R, CLARKE A, HURST R, and HANCOCK JT. 2001. Hydrogen peroxide and nitric oxide as signalling molecules in plants. The Journal of Experimental Botany 53, 1237–1247.
  • OVERMYER K, BROSCHE M, PELLINEN R, KUITTINEN T, TUOMINEN H, AHLFORS R, KEINANEN M, SAARMA M, SCHEEL D, and KANGASJARVI J. 2005. Ozone-induced programmed cell death in the Arabidopsis radical-induced cell death1 mutant. Plant Physiology 137: 1092–1104.
  • PANG N, and ZHANG FX. 2013. Hypoxia can damage nucleolar structure and inhibit its function on root-tip meristematiccells of common wheat. Acta Botanica Boreali-Occidentalia Sinica 33: 0507–0511.
  • PEROU CM, SORLIE T, EISEN MB, VAN DE RIJN M, JEFFREY SS, REES CA, POLLACK JR, ROSS DT, JOHNSEN H, AKSLEN LA,FLUGE O, PERGAMENSCHIKOV A, WILLIAMS C, ZHU SX, LONNINGPE, BORRESEN-DALE AL, BROWN PO, and BOTSTEIN D.2000. Molecular portraits of human breast tumours.Nature 406: 747–752.
  • RABALAIS NN, DIAZ RJ, LEVIN LA, TURNER RE, GILBERT D, and ZHANG J. 2010. Dynamics and distribution of naturaland human-caused hypoxia. Biogeosciences 7: 585–619.
  • SAIRAM RK, KUMUTHA D, and EZHILMATHI K. 2009. Waterlogging tolerance: nonsymbiotic haemoglobin-nitric oxide homeostasis and antioxidants. Current Science 96: 674–682.
  • SANGHERA GS, WANI SH, HUSSAIN W, and SINGH NB. 2011. Engineering cold stress tolerance in crop plants. Current Genomics 12: 30–43.
  • SATO S, and YAMADA M. 1996. Effect of hypoxia on nucleoli in excised root tips of Vicia faba. Cytologia 61: 209–214.
  • SERRANO I, ROMERO-PUERTAS MC, RODRIGUEZ-SERRANO M, SANDALIO LM, and OLMEDILLA A. 2012. Peroxynitrite mediates programmed cell death both in papillar cells and in selfincompatible pollen in the olive (Olea europaea L.). Journal of Experimental Botany 63: 1479–1493.
  • SHAK K, KUMAR RG, VERMA S, and DUBEY RS. 2001. Effect of cadmium on lipid peroxidation, superoxide anion generationand activities of antioxidant enzymes in growingrice seedlings. Plant Science 161: 1135–1144.
  • SILVA-CARDENAS RI, RICARD B, SAGLIO P, and HILL RD. 2003. Hemoglobin and hypoxic acclimation in maize root tips. Russian Journal of Plant Physiology 50: 821–826.
  • SIMON HU, HAJ-YEHIA A, and LEVI-SCHAFFER F. 2000. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis 5: 415–418.
  • THORDAL-CHRISTENSEN H, ZHANG Z, WEI Y, and COLLINGE DB. 1997. Subcellular localization of H2O2 in plant, H2O2accumulation in papillae and hypersensitive responseduring the barley-powdery mildew interaction. PlantJournal 11:1187–1194.
  • VAN BREUSEGEM F, and DAT JF. 2006. Reactive oxygen species in plant cell death. Plant Physiology 141: 384–390.
  • VAN DOORN WG, and WOLTERING EJ. 2005. Many ways to exit? Cell death categories in plants. Trends in Plant Science10: 117–122.
  • VAN DOORN WG, BEERS EP, DANGL JL, FRANKLIN-TONG VE, GALLOIS P, HARA-NISHIMURA I, JONES AM, KAWAI-YAMADA M,
  • LAM E, MUNDY J, MUR LA, PETERSEN M, SMERTENKO A, TALIANSKY M, VAN BREUSEGEM F, WOLPERT T, WOLTERING EJ, ZHIVOTOVSKY B, and BOZHKOV PV. 2011. Morphological classification of plant cell deaths. Cell Death and Differentiation 18: 1241–1246.
  • VIROLAINEN E, BLOKHINA O, and FAGERSTEDT K. 2002. Ca(2+)- induced high amplitude swelling and cytochrome crelease from wheat (Triticum aestivum L.) mitochondriaunder anoxic stress. Annals of Botany 90: 509–516.
  • ZHAO YG, and XU JX. 2001. Mitochondria, reactive oxygen species and apoptosis. Progress in Biochemistry and Biophysics 28: 168–171.

Typ dokumentu

Bibliografia

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