Aktywność krwinkowej dysmutazy ponadtlenkowej i peroksydazy glutationowej oraz surowicze stężenie witaminy E u psów z zaćmą związaną z wiekiem

• Autorzy: Madany J. , Abramowicz B. , Milczak A. , Winiarczyk D. , Wrzesniewska K.

Warianty tytułu

EN

Activities of blood superoxide dismutase, glutathione peroxidase and serum vitamin E level in dogs with age-related cataract

• Języki publikacji: PL

Abstrakty

EN

Purpose: Oxidative mechanisms play a major role in the etiology and pathogenesis of cataract, especially age-related cataract (ARC). Our study aimed to investigate the serum vitamin E level and the activities of systemic antioxidant enzymes: superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the blood of dogs with age-related cataract but without any general diseases. Material and methods: The population examined consisted of 40 dogs of various breeds and both sexes, aged 10-16 years, diagnosed with age-related cataract. Cataract was diagnosed by complete ocular examination. Blood was collected during clinical examination from the external jugular vein in dogs weighing less than 20 kg or from the small saphenous vein in dogs weighing over 20 kg. The control group consisted of 10 healthy dogs of different breeds and sexes, aged 8-12 years, without cataract. SOD and GPX were measured by a colorimetric reagent kit Randox SOD and Randox GPX. The level of vitamin E was determined using a Perkin-Elmer LS 30 luminescent spectrometer. This apparatus determines the vitamin E level by the fluorometric method of Hansen and Warwick . Results: The results showed a significantly lower activity of SOD as well as a lower activity of GPX and a lower level of the vitamin E in dogs with ARC compared to healthy dogs. Conclusions: The results obtained indicate a weakening of the general antioxidant defence system in the course of ARC in dogs. These results suggest that such conditions may have effect on the development of canine age-related cataract and are consistent with the free radical theory of age-related cataract development.

Słowa kluczowe

PL

psy; choroby oczu; zacma starcza; zwierzeta chore; badania krwi; dysmutaza ponadtlenkowa; peroksydaza glutationowa; aktywnosc enzymatyczna; surowica krwi; stezenie witaminy E

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2019
• Tom: 75
• Numer: 10
• Opis fizyczny: s.590-592,tab.,bibliogr.

Twórcy

autor

Madany J.

• Katedra i Klinka Chorób Wewnętrznych Zwierząt, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Głęboka 30, 20-612 Lublin

autor

Abramowicz B.

• Katedra i Klinka Chorób Wewnętrznych Zwierząt, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Głęboka 30, 20-612 Lublin

autor

Milczak A.

• Katedra i Klinka Chorób Wewnętrznych Zwierząt, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Głęboka 30, 20-612 Lublin

autor

Winiarczyk D.

• Katedra i Klinka Chorób Wewnętrznych Zwierząt, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Głęboka 30, 20-612 Lublin

autor

Wrzesniewska K.

• Katedra i Klinka Chorób Wewnętrznych Zwierząt, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Głęboka 30, 20-612 Lublin

Bibliografia

• Ates N. A., Yildrim O., Tamer L., Unlu A., Ercan B., Muslu N., Kanik A., Hatungil R., Atik U.: Plasma catalase activity and malondialdehyde level in patients with cataract. Eye 2004, 18, 785-788.
• Barros P. S. M., Angelotti A. C., Nobre F., Morales A., Fantoni D. T., Barros S. B. M.: Antioxidant profile of cataractous english cocker spaniels. Vet. Ophtalm. 1999, 2, 83-86.
• Chandrasena L. G., Chackrewarthy S., Perera P. T., de Silva D.: Erythrocyte antioxidant enzymes in patients with cataract. Ann. Clin. Lab. Sci. 2006, 36, 201-204.
• Chang D., Zhang X., Rong S., Sha Q., Liu P., Han T., Pan H.: Serum antioxidative enzymes level and antioxidative stress products in age-related cataract patients. Oxid. Med. Cell. Longev. 2013, 1-7.
• Congdon N., O’Colmain B., Klaver C. C., Klein R., Munoz B., Friedmann D. S., Kempen J., Taylor H. R., Mitchell P.: Causes and prevalence of visual impairment among adults in the United States. Arch. Ophthalmol. 2004, 122, 477-485.
• Cui Y.-H., Jing Ch.-X., Pan H.-W.: Association of blood antioxidants and witamins with risk of age-related cataract: a metaanalysis of observational studies 1-3. Am. J. Clin. Nutr. 2013, 98, 778-786.
• Gelatt K. N., MacKay E. O.: Prevalence of primary breed-related cataracts in the dog in North America. Vet. Ophtalmol. 2005, 8, 101-111.
• Inal M. E., Kanbak G., Sunal E.: Anioxidant enzyme activities and malondialdehyde levels related to ageging. Clin. Chim. Acta 2001, 305, 75-80.
• Kaur J., Kukreja S., Kaur A., Malhotra N., Kaur R.: The oxidative stress in cataract patients. J. Clin. Diagn. Res. 2012, 6, 1629-1632.
• Lin D., Barnett M., Grauer L., Robben J., Jewell A., Takemoto L., Takemoto D. J.: Expression of superoxide dismutase in whole lens prevents cataract formation. Mol. Vis. 2005, 11, 853-858.
• Madany J.: Serum malondialdehyde level and activity of total antioxidant status of dogs with age-related cataract. Pol. J. Vet. Sci. 2016, 19, 429-431.
• Madany J., Miczak A., Abramowicz B.: Malondialdehyde content and the activity of the total antioxidant status in the aqueous humor of dogs with senile cataract. Med. Weter. 2014, 70, 636-637.
• Maurya O. P., Mohanty L., Bhaduri G., Chandra A.: Role of anti-oxidant enzymes superoxide dismutase and catalase in the development of cataract: study of serum levels in patients with senile and diabetic cataracts. J. Indian Med. Assoc. 2006, 104, 394, 396-397.
• Micelli-Ferrari T., Vendemiale G., Grattagliano I., Boscia F., Arnese L., Altomare E., Cardia L.: Role of lipid peroxidation in the pathogenesis of myopic and senile cataract. Brit. J. Ophthalmol. 1996, 80, 848-843.
• Ofri R.: Soczewka, [w:] Maggs D. J., Miller P. E., Ofri R.: Okulistyka weterynaryjna Slattera. Elsevier, Wrocław 2008, s. 284-304.
• Ottonello S., Foroni C., Carta A., Petrucco S., Maraini G.: Oxidative stress and age-related cataract. Ophtalmologica 2000, 214, 78-85.
• Simeonova G. P., Nedev V. S., Sleiman M. U.: Evidence of local oxidative stress in canine senile cataract. Revue Med. Vet. 2018, 169, 7-9, 173-179.
• Taylor A., Jacques P. F., Epstein E. M.: Relations among aging, antioxidant status and cataract. Am. J. Clin. Nutr. 1995, 63, 1439S-1447S.
• Tomsic K., Seliskar A., Lukanc B., NemecSwete A.: Plasma total antioxidant capacity and activities of blood glutathione peroxidase and superoxide dismutase determined in healthy dogs by using commercially available kits. Acta Vet. Beograd 2016, 66, 534-548.
• Truscott R. J.: Age-related nuclear cataract oxidation is the key. Exp. Eye Res. 2005, 80, 709-725.
• Vashist P., Talwar B., Gogoi M., Maraini G., Camparini M., Ravindran R. D., Murthy G. V., Fitzpatrick K. E., John N., Chakravarthy U. et al.: Prevalence of cataract in an older population in India: the India study of age – related eye disease. Ophthalmology 2011, 118, 272-278.
• Vinson J. A.: Review. Oxidative stress in cataracts. Pathophysiology 2006, 13, 151-162.
• Wiliam C. G., Glynn R. J., Manson J. E., MacFadyen J., Bubes V., Schwartz M., Buring J. E., Sesso H. D., Gaziano J. M.: Effects of multivitamin supplement on cataract and age-related macular degeneration in a randomized trial of male physicians. Ophthalmology 2014, 121, 525-534.
• Wiliams D. L., Heath M. F., Wallis C.: Prevalence of canine cataract: preliminary results of a cross-sectional study. Vet. Ophtlmol. 2004, 7, 29-35.
• Yang W., Yu W., Li Z.: The study on superoxide dismutase and trace element in patients with senile cataract. Eye Science 2000, 16, 246-248.

Identyfikatory

DOI

10.21521/mw.6247