Bakteryjny rak pomidora - problem nadal aktualny

• Autorzy: Kamasa J. , Krawczyk K. , Mackowiak-Sochacka A. , Zwolinska A.

Warianty tytułu

EN

Bacterial canker of tomato - still actual problem

• Języki publikacji: PL

Abstrakty

EN

The aim of presented study was the monitoring of the occurence of Cmm in 2006–2010. During the studied period, 97 tomato plants were tested. In 33 cases Cmm was detected. All plants diagnosed positivly showed disease symptoms, which appeared on tomato plants at the fruit setting stage. Characteristic unilateral wilting and necrosis were observed. 2–5 days after bacteria isolation on the NCP-88 medium, typical Cmm convex ,cylindrical colonies from light yellow to a milky yellow were observed. The pathogenicity test on tomato seedlings was additionally performed. Usually seven days after inoculation, typical symptoms of tomato bacterial cancer – unilateral wilting of leaves, appeared. In order to fulfill the Koch postulates, the bacteria were reisolated from artificially inoculated tomato seedlings showing unilateral wilting. Their identity was confirmed by PCR reaction with Cmm specific primers pairs: PSAR/PSA-4 or Cmm4/Cmm5. The presence of reaction products with a length of 270 nt for the first and 614 nt for the second pair of primers confirmed the identification. As a positive control, the Cmm isolate from the collection of the Department of Virology and Bacteriology, The Institute of Plant Protection – National Research Institute was used.

• Wydawca: -
• Czasopismo: Progress in Plant Protection
• Rocznik: 2011
• Tom: 51
• Numer: 3
• Opis fizyczny: s.1155-1158,bibliogr.

Twórcy

autor

Kamasa J.

• Instytut Ochrony Roślin - Państwowy Instytut Badawczy, ul.Władysława Węgorka 20, 60-318 Poznań

autor

Krawczyk K.

• Instytut Ochrony Roślin - Państwowy Instytut Badawczy, ul.Władysława Węgorka 20, 60-318 Poznań

autor

Mackowiak-Sochacka A.

• Instytut Ochrony Roślin - Państwowy Instytut Badawczy, ul.Władysława Węgorka 20, 60-318 Poznań

autor

Zwolinska A.

• Instytut Ochrony Roślin - Państwowy Instytut Badawczy, ul.Władysława Węgorka 20, 60-318 Poznań

Bibliografia

• Balows A., Trüper H.G., Dworkin M., Harder W., Schleifer K.H. 1992. The Procaryotes 2nd ed. Vol. I and II. Springer-Verlag, New York, Berlin, 1663 pp.
• Dreier J., Bermpohl A., Eichenlaub R. 1995. Southern hybridization and PCR for specific detection of phytopathogenic Clavibacter michiganensis subsp. michiganensis. Phytopathology 85: 462–468.
• Dziennik Ustaw 2008. Rozporządzenie Ministra Rolnictwa i Rozwoju Wsi z dnia 21 lutego 2008 r. w sprawie zapobiegania wprowadzaniu i rozprzestrzenianiu się organizmów kwarantannowych. Dz.U. Nr 46, poz. 272 z dnia 17.03.2008.
• Gartemann K.H., Bekel T., Burger A., Engemann J., Flügel M., Gaigalat L., Goesmann A., Gräfen I., Kalinowski J., Kaup O., Kirchner O., Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rückert C., Schneiker S., Zellermann E.M., Pühler A., Eichenlaub R., Kaiser O., Bartels D. 2008. The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity. J. Bacteriol. 190 (6): 2138–2149.
• Gitaitis R.D. 1990. Induction of a hypersensitivelike reaction in four-o’clock by Clavibacter michiganensis subsp. michignensis. Plant Dis. 74 (1): 58–60.
• Huang R., Tu J.C. 2001. Effects o nutrient solution pH on the survival and transmission of Clavibacter michiganensis ssp. michiganensis in hydroponically grown tomatoes. Plant Pathol. 50: 503–508.
• León de L., Siverio F., Rodríguez A. 2006. Detection of Clavibacter michiganensis subsp. michiganensis in tomato seeds using immunomagnetic separation. J. Microbiol. Meth. 67: 141–149.
• Löffler H.J.M., Van der Bulk R.W., Kerckhoffs D., Purimahua C., Lindhout W.H. 1989. A fast screening method for the detection of resistance Clavibacter michiganense in tomato. p. 957–963. In: „Plant Pathogenic Bacteria”. Proc. 7th Int. Conf. Plant Path. Bact. Hungary, Budapest, 11–17.06.1989, 1061 pp.
• Pastrik K.-H., Rainey F.A. 1999. Identification and differentiation of Clavibacter michiganensis subspecies by polymerase chain reaction-based techniques. J. Phytopathol. 47: 687–693.
• Schaad N.W., Jones J.B., Chun W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3rd ed. The APS, St. Paul, MN, 373 pp.
• Sherf A.F., Macnab A.A. 1986. Vegetable Diseases and Their Control. 2nd ed. Wiley, New York, 736 pp.
• Xiulan Xu., Miller S.A., Baysal-Gurel F., Gartemann K.H., Eichenlaub R., Rajashekara G. 2010. Bioluminescence Imaging of Clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants. Appl. Env. Microbiol. 76: 978–988.