Częstotliwość izolacji Campylobacter spp. z klatek transportowych podczas transportu brojlerów kurzych do rzeźni

• Autorzy: Gomolka-Pawlicka M. , Pastuszak-Frak M. , Uradzinski J. , Migowska-Calik A. , Wysok B. , Lachowicz T.

Warianty tytułu

EN

Incidence of Campylobacter spp. in transport cages during the transport of broilers to the slaughterhouse

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to determine the frequency of the occurrence of Campylobacter spp. on the surface of transport containers during various stages of the transportation of broilers to slaughterhouses located in north-eastern Poland. Sampling, sample preparation, and microbiological testing were performed according to the method given in Standard PN EN ISO 10 272-1: 2007. In the last day of the birds’ breeding cycle, 20 swabs were taken from internal surfaces of the crates: 10 in the first phase of transport (before loading the birds) and 10 in the second phase of transport, just before unloading the broilers at the slaughterhouse. The investigation concerned the transport of birds from 16 breeding cycles, from four farms, during nine consecutive seasons. A total of 320 samples were tested (160 were collected at the farms and 160 in three poultry slaughterhouses). The incidence of Campylobacter spp. in the first phase of transport depended on the time of the year and amounted to 7.5% in summer, 25% in spring and winter, and 35% in autumn. In the second phase of transport, the incidence significantly increased, and was the highest in summer and autumn (75%), slightly lower in spring (72.5%), and the lowest in winter (35%). An increase in the number of positive results compared to the results from the first phase of transport depended on the season, amounting to 67.5% in summer, 47% in spring, 40% in autumn, and 10% in winter Biochemical tests confirmed the presence of Campylobacter jejuni, and the results of a PCR analysis showed complete conformity with the results obtained in the biochemical tests. The results indicate a high incidence of Campylobacter spp. on the surface of transport containers, which is mainly due to inadequate hygiene of the cages brought to the farms on the day of the birds’ transport to the slaughterhouse. This highlights the need to apply the strictest hygiene regimes in farms and to improve the effectiveness of the cleaning and disinfecting of cages, regardless of the time of the year. Negligence in this area favors the spread of Campylobacter spp. among the birds just before the slaughter, which increases the risk of meat contamination.

Słowa kluczowe

PL

drob; brojlery kurze; transport zwierzat; klatki transportowe; zanieczyszczenia bakteryjne; Campylobacter; wystepowanie; izolacja; czestotliwosc wystepowania

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2014
• Tom: 70
• Numer: 02
• Opis fizyczny: s.107-111,rys.,bibliogr.

Twórcy

autor

Gomolka-Pawlicka M.

• Katedra Weterynaryjnej Ochrony Zdrowia Publicznego, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-718 Olsztyn

autor

Pastuszak-Frak M.

• Katedra Weterynaryjnej Ochrony Zdrowia Publicznego, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-718 Olsztyn

autor

Uradzinski J.

• Katedra Weterynaryjnej Ochrony Zdrowia Publicznego, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-718 Olsztyn

autor

Migowska-Calik A.

• Katedra Weterynaryjnej Ochrony Zdrowia Publicznego, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-718 Olsztyn

autor

Wysok B.

• Katedra Weterynaryjnej Ochrony Zdrowia Publicznego, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-718 Olsztyn

autor

Lachowicz T.

• Katedra Cyfryzacji, Wydział Prawa i Administracji, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul. Oczapowskiego 12B, 10-719 Olsztyn

Bibliografia

• 1. Asselt E. D. van, Jacobs-Reitsma W. F., Brakel R. van, Voet H. van der, Fels-Klerx H. J. van der.: Campylobacter prevalence in the broiler supply chain in the Netherlands. Poult. Sci. 2008, 87, 2166-2172.
• 2. Berrang M. E., Hofacre C. L., Meinersmann R. J.: Forced hot air to dry feces and kill bacteria on transport cage flooring. J. Appl. Poult. Res. 2011, 20, 567-572.
• 3. Berrang M. E., Northcutt J. K.: Use of water spray and extended drying time to lower bacterial numbers on soiled flooring from broiler transport coops. Poult. Sci. 2005a, 84, 1797-1801.
• 4. Berrang M. E., Northcutt J. K.: Water Spray and Immersion in Chemical Sanitizer to Lower Bacterial Numbers on Broiler Transport Coop Flooring. J. Appl. Poult. Res. 2005b, 14, 315-321.
• 5. Berrang M. E., Northcutt J. K., Cason J. A.: Recovery of Campylobacter from broiler feces during extended storage of transport cages. Poult. Sci. 2004, 83, 1213-1217.
• 6. Berrang M. E., Northcutt J. K., Fletcher D. L., Cox N. A.: Role of Dump Cage Fecal Contamination in the Transfer of Campylobacter to Carcasses of Previously Negative Broilers. J. Appl. Poult. Res. 2003, 12, 190-195.
• 7. Byrd J. A., Corrier D. E., Hume M. E., Bailey R. H., Stanker L. H., Hargis B. M.: Incidence of Campylobacter in crops of preharvest market-age broiler chickens. Poultry Sci. 1998, 77, 1303-1305.
• 8. Corry J. E. L., Atabay H. I.: Poultry as a source of Campylobacter and related organisms. J. Appl. Microbiol. 2001, 90, 96-114.
• 9. Daczkowska-Kozon E.: Epidemiologia zakażeń wywoływanych przez pałeczki z rodzaju Campylobacter. I. Zbiorniki i organizmy wodne jako rezerwuar Campylobacter sp. Post. Mikrobiol. 2002, 41, 133-146.
• 10. Denis M., Chidaine B., Laisney M. J., Kempf I., Rivoal K., Mégraud F., Fravalo P.: Comparison of genetic profiles of Campylobacter strains isolated from poultry, pig and Campylobacter human infections in Brittany, France. Pathol. Biol. 2009, 57, 23-29.
• 11. EFSA The Community Summary Report 2013.
• 12. Franchin P. R., Aidoo K. E., Batista C. R. V.: Sources of poultry meat contamination with thermophilic Campylobacter before slaughter. Braz. J. Microbiol. 2005, 36, 157-162.
• 13. Hansson I., Ederoth M., Andersson L., Vågsholm I., Olsson Engvall E.: Transmission of Campylobacter spp. to chickens during transport to slaughter. J. Appl. Microbiol. 2005, 99, 1149-1157.
• 14. Hastings R., Colles F. M., McCarthy N. D., Maiden M. C. J., Sheppard S. K.: Campylobacter genotypes from poultry transportation crates indicate a source of contamination and transmission. J. Appl. Microbiol. 2011, 110, 266-276.
• 15. Herman L., Heyndrickx M., Grijspeerdt K., Vandekerchove D., Rollier I., De Zutter L.: Routes for Campylobacter contamination of poultry meat: epidemiological study from hatchery to slaughterhouse. Epidemiol. Infect. 2003, 131, 1169-1180.
• 16. Huat J. T. Y., Aziz S. A., Abu J., Ghazali F. M., Chilek T. Z. T., Ahmad N., Sandra A., Nishibuchi M., Radu S.: Thermophilic Campylobacter spp. occurrence on chickens at farm, slaughter house and retail. Int. J. Poult. Sci. 2010, 9, 134-138.
• 17. Linton A. H., Hinton M. H.: Prevention of microbial contamination of red meat in the ante mortem phase: epidemiological aspects, [w:] Smulders F. J. M. (ed), Elimination of pathogenic organisms from meat and poultry. Elsevier Science Publishers, New York 1986, 9-23.
• 18. Miflin J. K., Templeton J. M., More S. J.: Epidemiological studies of Campylobacter colonisation of broiler. f locks in south east Queensland. Proc. Aust. Poult. Sci. Sym. 2001, 13, 140-143.
• 19. Mulder R. W. A. W.: Impact of transport on the incidence of human pathogens in poultry. Misset World Poult. 1996, 12, 18-19.
• 20. Newell D. G., Shreeve J., Toszeghy M., Domingue G., Bull S., Humphrey T., Mead G.: Changes in the carriage of Campylobacter strains by poultry carcasses during abattoir processing. Appl. Environ. Microbiol. 2001, 67, 2636-2640.
• 21. Nor Faiza S., Saleha A. A., Jalila A., Fauziah N.: Occurrence of Campylobacter and Salmonella in ducks and duck eggs in Selangor, Malaysia. Trop. Biomed. 2013, 30, 155-158.
• 22. Northcutt J. K., Berrang M. E.: Influence of a Chicken Transport Cage-Washing System on Wastewater Characteristics and Bacteria Recovery from Cage Flooring. J. Appl. Poult. Res. 2006, 15, 457-463.
• 23. Northcutt J. K., Berrang M. E., Dickens J. A., Fletcher D. L., Cox N. A.: Effect of broiler age, feed withdrawal, and transportation on levels of coliforms, Campylobacter, Escherichia coli and Salmonella on carcasses before and after immersion chilling. Poult. Sci. 2003, 82, 169-173.
• 24. PN-EN ISO 10 272 – 1: 2007 Mikrobiologia żywności i pasz. Horyzontalna metoda wykrywania obecności i oznaczania liczby Campylobacter spp.
• 25. Ridley A., Morris V., Gittins J., Cawthraw S., Harris J., Edge S., Allen V.: Potential sources of Campylobacter infection on chicken farms: contamination and control of broiler-harvesting equipment, vehicles and personnel. J. Appl. Microbiol. 2011, 111, 233-244.
• 26. Rosenquist H., Sommer H. M., Nielsen N. L., Christensen B. B.: The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter. Int. J. Food Microbiol. 2006, 108, 226-232.
• 27. Sheppard S. K., Dallas J. F., MacRae M., McCarthy N. D., Sprostonb E. L., Gormley F. J., Strachan N. J. C., Ogden I. D., Maiden M. C. J., Forbes K. J.: Campylobacter genotypes from food animals, environmental sources and clinical disease in Scotland 2005/6 Int. J. Food Microbiol. 2009, 134, 96-103.
• 28. Slader J., Domingue G., Jørgensen F., McAlpine K., Owen R. J., Bolton F. J., Humphrey T. J.: Impact of Transport Crate Reuse and of Catching and Processing on Campylobacter and Salmonella Contamination of Broiler Chickens. Appl. Environ. Microbiol. 2002, 68, 713-719.
• 29. Stern N. J., Clavero M. R. S., Bailey J. S., Cox N. A., Robach M. C.: Campylobacter spp. in broilers on the farm and after transport. Poult. Sci. 1995, 74, 937-941.
• 30. Szczepańska B., Klawe J. J., Szady-Grad M., Jurgoński A., Andrzejewska M.: Występowanie bakterii z rodzaju Campylobacter u drobiu w trakcie procesu ubojowego. Probl. Hig. Epidemiol. 2007, 88, 78-83.
• 31. Uradziński J.: Rola Campylobacter spp. w patologii zwierząt i ludzi, [w:] Szweda W., Siwicki A. K. (red.): Epizootyczne aspekty monitorowania i zwalczania zoonoz w Polsce i Unii Europejskiej. Wyd. Edycja, Olsztyn 2007, 27-36.
• 32. Vellinga A., Van Loock F.: The Dioxin Crisis as Experiment To Determine Poultry-Related Campylobacter Enteritis. Emerg. Infect. Dis. 2002, 8, 19-22.
• 33. Wesley I. V., Rostagno M., Hurd H. S., Trampel D. W.: Prevalence of Campylobacter jejuni and Campylobacter coli in Market-Weight Turkeys On-Farm and at Slaughter. J. Food Prot. 2009, 72, 43-48.
• 34. Whyte P., Collins J. D., McGill K., Monahan C., O’Mahony H.: The Effect of Transportation Stress on Excretion Rates of Campylobacters in Market-Age Broilers. Poult. Sci. 2001, 80, 817-820.
• 35. Workman S. N., Mathison G. E., Lavoie M. C.: An investigation of sources of Campylobacter in a poultry production and packing operation in Barbados. Int. J. Food Microbiol. 2008, 121, 106-111.