An efficient system for regenerating Taraxacum pieninicum Pawl. from seedling explants

• Autorzy: Trejgell A. , Chernetskyy M. , Podlasiak J. , Tretyn A.
• Języki publikacji: EN

Abstrakty

EN

An efficient micropropagation system for Taraxacum pieninicum using seedling explants germinated in vitro is described. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from several-day-old seedlings. The highest response, 100% frequency with 12.3 axillary shoots/explant, was from shoot tips on medium supplemented with 0.5 mg L-1 BA and 0.05 mg L-1 NAA. In subsequent subcultures the number of shoots was significantly higher on all explants cultured on medium containing 0.25 and 0.5 mg L-1 BA, and the multiplication rate was highest (20 shoots/explant) in the 4th passage. Shoots rooted on MS and 1/2 MS medium; the highest rooting frequency was 90% and the highest number of roots 2.7/shoot. Rooted plants showed 96.2% survival in sterile soil:sand, and 100% survival in hydroponic culture. Regenerated plants flowered in the second year after acclimatization and yielded viable seeds. This protocol for obtaining complete plants through micropropagation may prove useful for conservation of the genetic resources of this and other endangered species.

Słowa kluczowe

EN

Taraxacum pieninicum; seedling explant; fertile plant; in vitro culture; multiple shoot; benzylaminopurine; hydroponic culture; genetic resource; plant regeneration; plant conservation

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2013
• Tom: 55
• Numer: 1
• Opis fizyczny: p.73-79,fig.,ref.

Twórcy

autor

Trejgell A.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Torun, Poland

autor

Chernetskyy M.

• Botanical Garden of Maria Curie-Sklodowska University, Slawinkowska 3, 20-810 Lublin, Poland

autor

Podlasiak J.

• Polish Acadamy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973 Warsaw, Poland

autor

Tretyn A.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Torun, Poland

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Uwagi

PL

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