Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2007 | 29 | 2 |
Tytuł artykułu

Benzopyrans in Hypericum polyanthemum Klotzsch ex Reichardt cultured in vitro

Treść / Zawartość
Warianty tytułu
Języki publikacji
Hypericum polyanthemum Klotzsch ex Reichardt, an endemic species of Southern Brazil, was micropropagated on MS medium supplemented with 1.78 µM BAP. Shoot proliferation and rooting was achieved on hormone-free medium and plantlets survived acclimatization. The bioactive compounds: 6-isobutyryl-5,7-dimethoxy-2,2-dimethylbenzopyran (HP1), 7-hydroxy-6-isobutyryl-5-methoxy2,2-dimethyl-benzopyran (HP2) and 5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl-benzopyran (HP3) were quantified in the leaves, stems and roots of propagated and acclimatized plantlets and compared with the field-grown plants. The HPLC analysis revealed that the three benzopyrans are accumulated in the aerial parts and the concentration varied with the age of the plant whereas the roots were capable of accumulating only HP3. Greatest yield of HP1 (7.12 mg/g DW) was quantified in the leaves of the acclimatized plantlets, whereas the flowers of the plants from natural habitat displayed higher amounts of HP2 (11.04 mg/g DW) and HP3 (13.99 mg/g DW).
Słowa kluczowe
Opis fizyczny
  • Programa de Pos Graduacao em Cinencias Farmaceuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, 90610-000 Porto Alegre, Brazil
  • Programa de Pos Graduacao em Cinencias Farmaceuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, 90610-000 Porto Alegre, Brazil
  • Programa de Pos Graduacao em Cinencias Farmaceuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, 90610-000 Porto Alegre, Brazil
  • Programa de Pos Graduacao em Cinencias Farmaceuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, 90610-000 Porto Alegre, Brazil
  • Abreu IN, Mazzafera P (2005) Effect of water and temperature stress on the content of active constituents of Hypericum brasiliense Choisy. Plant Physiol Biochem 43:241–248
  • Agostinis PA, Vantieghem A, Merlevede W, de Witte P (2002) Hypericin in cancer treatment: more light on the way. Int J Biochem Cell Biol 34:221–241
  • Briskin DP, Gawienowski MC (2001) Differential effects of light and nitrogen on production of hypericins and leaf glands in Hypericum perforatum. Plant Physiol Biochem 39:1075–1081
  • Cardoso MA, Oliveira DE (1996) Tissue culture of Hypericum brasiliense Choisy: shoot multiplication and callus induction. Plant Cell Tiss Org Cult 44:91–94
  • Cellárová E, Kimáková K, Brutovská R (1992) Multiple shoot formation and phenotypic changes of R₀ regenerants in Hypericum perforatum L. Acta Biotechnol 12:445–452
  • Chatterjee SS, Nöldner M, Koch E, Erdelmeier E (1998) Antidepressant activity of Hypericum perforatum and hyperforin: the neglected possibility. Pharmacopsychiatry 31:7–15
  • Dall’Agnol R, Ferraz A, Bernardi AP, Albring D, Nor C, Sarmento L, Lamb L, Hass M, von Poser GL, Schapoval EES (2003) Antimicrobial activity of some Hypericum species. Phytomedicine 10:141–147
  • Daudt R, Poser GLV, Staats C, Neves G, Rates SMK (2000) Screening for the antidepressant activity of some species of Hypericum from South Brazil. Phytother Res 14:344–346
  • Diwu Z (1995) Novel therapeutic and diagonistic applications of hypocrellins and hypericins. Photochem Photobiol 61:529–539
  • Ferraz ABF, Bordignon S, Staats C, Schripsema J, von Poser GL (2001) Benzopyrans from Hypericum polyanthemun. Phytochemistry 57:1227–1230
  • Ferraz ABF, Grivicich I, von Poser GL, Faria DH, Kayser GB, Schwartsmann G, Henriques AT, da Rocha AB (2005a) Antitumor activity of three benzopyrans isolated from Hypericum polyanthemun. Fitoterapia 76:210–215
  • Ferraz ABF, Faria DH, Benneti MN, da Rocha AB, Schwartsmann G, Henriques AT, von Poser GL (2005b) Screening for antiproliferative activity of six southern Brazilian species of Hypericum. Phytomedicine 12:112–115
  • Gnerre C, von Poser GL, Ferraz AC, Viana A, Testa B, Rates SMK (2001) Monoamine oxidase inhibitory activity of some Hypericum species native to South Brazil. J Pharm Pharmacol 53:1273–1279
  • Jiménes CR (1980) Hipericaceaes. Flora Ilustrada Catarinense. Editora IOESC, Florianópolis, pp 3–32
  • Magii F, Ferretti G, Pocceshi N, Menghini L, Ricciutelli M (2004) Morphological, histochemical and phytochemical investigation of the genus Hypericum of the Central Italy. Fitoterapia 75:702–711
  • McCoy JA, Camper ND (2002) Development of a micropropagation protocol for St. John’s Wort (Hypericum perforatum L.). HortScience 37:978–980
  • Mentz LA, Lutzemberger LC, Schenkel EP (1997) Da flora medicinal do Rio Grande do Sul: notas sobre a obra literária de D’Ávila. Cad Farm 13:25–47
  • Moura M (1998) Conservation of Hypericum foliosum Aiton, na endemic Azorean species, by micropropagation. In Vitro Cell Dev Biol Plant 34:244–248
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Murch SJ, Rupasinghe HPV, Saxena PK (2002) An in vitro and hydroponic growing system for hypericin, pseudohypericin, and hyperforin production of St. John’s wort (Hypericum perforatum L. cv New Stem). Planta Med 68:1108–1112
  • Öztürk Y (1997) Testing the antidepressant effects of Hypericum species on animal models. Pharmacopsychiatry 40:125–128
  • Pasqua G, Avato P, Monacelli B, Santamaria AR, Argentieri MP (2003) Metabolites in cell suspension cultures, calli, and in vitro regenerated organs of Hypericum perforatum cv. Topas Plant Sci 165:977–982
  • Robson NKB. (1990) Studies in the genus Hypericum L. (Guttiferae) 8. Sections 29. Brathys (part 2) and 30. Trigynobrathys. Bull Br Mus 20:1–151
  • Singer AS, Wonnemann M, Muller W (1998) Hyperforin, a major antidepressant constituent of St. John’s Wort, inhibits serotonin uptake by elevating free intracellular Na⁺¹. J Pharmacol Exp Ther 290:1363–1368
  • Sokal RR, Rohlf FJ (1981) Biometry. Freeman, San Francisco, p 859
  • Southwell IA, Bourke CA (2001) Seasonal variation in hypericin content of Hypericum perforatum L. (St. John’s wort). Phytochemistry 56:437–441
  • Takel’ova D, Repcak M, Zemkova E, Toth J (2000) Quantitative changes in dianthrones, hyperforin and flavonoids in the flower ontogenesis of Hypericum perforatum. Planta Med 66:778–780
  • Tamas LA (1987) Hormonal regulation of apical dominance. In: Davies PJ (ed) Plant hormones and their role in plant growth and development. Martinus Nijhoff, Dordrecht, pp 393–410
  • Viana AF, Heckler AP, Fenner R, Rates SMK (2003) Analgesic activity of Hypericum caprifoliatum and Hypericum polyanthemum (Guttiferae). Braz J Med Biol Res 36:631–634
  • Vyas AV, Mulchandani NB (1980) Biosynthesis of precocenes –I and –II, anti-juvenile hormones. Phytochemistry 19:2597–2598
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.